Justicidin B
Based on 1 Customer Validation
Justicidin B is a potent anticancer lignan and proapoptotic agent. Justicidin B is also a bone resorption inhibitor, and has strong antiviral, fungicidal, antiprotozoal effects. Justicidin B significantly inhibits platelet aggregation.
For research use only. We do not sell to patients.
- Purity: 99.75%
- CAS No.: 17951-19-8
- Formula: C21H16O6
- Molecular Weight:364.35
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Parasite Isoforms
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Biological Activity
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Trypanosoma |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A2058 | IC50 |
8.9 μM
Compound: 2
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Cytotoxicity against human A2058 cells incubated for 24 hrs by AlamarBlue staining based analysis
Cytotoxicity against human A2058 cells incubated for 24 hrs by AlamarBlue staining based analysis
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[PMID: 36857518] |
| A549 | IC50 |
11.4 μM
Compound: 2
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Cytotoxicity against human A549 cells incubated for 24 hrs by AlamarBlue staining based analysis
Cytotoxicity against human A549 cells incubated for 24 hrs by AlamarBlue staining based analysis
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[PMID: 36857518] |
| BGC-823 | IC50 |
0.18 μM
Compound: 2
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Cytotoxicity against human BGC-823 cells assessed as reduction in cell viability measured after 4 days by MTT colorimetric assay
Cytotoxicity against human BGC-823 cells assessed as reduction in cell viability measured after 4 days by MTT colorimetric assay
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[PMID: 36857518] |
| Calu-1 | IC50 |
18.5 μM
Compound: 2
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Cytotoxicity against human Calu-1 cells incubated for 24 hrs by AlamarBlue staining based analysis
Cytotoxicity against human Calu-1 cells incubated for 24 hrs by AlamarBlue staining based analysis
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[PMID: 36857518] |
| EBC-1 | IC50 |
63.2 μM
Compound: 2
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Cytotoxicity against human EBC-1 cells incubated for 24 hrs by AlamarBlue staining based analysis
Cytotoxicity against human EBC-1 cells incubated for 24 hrs by AlamarBlue staining based analysis
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[PMID: 36857518] |
| HepG2 | IC50 |
16.8 μM
Compound: 2
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Cytotoxicity against human HepG2 cells incubated for 24 hrs by AlamarBlue staining based analysis
Cytotoxicity against human HepG2 cells incubated for 24 hrs by AlamarBlue staining based analysis
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[PMID: 36857518] |
| HT-29 | IC50 |
5.9 μM
Compound: 2
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Cytotoxicity against human HT-29 cells incubated for 24 hrs by AlamarBlue staining based analysis
Cytotoxicity against human HT-29 cells incubated for 24 hrs by AlamarBlue staining based analysis
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[PMID: 36857518] |
| MONO-MAC-6 | IC50 |
2.5 μM
Compound: 2
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Cytotoxicity against human MONO-MAC-6 cells incubated for 24 hrs by AlamarBlue staining based analysis
Cytotoxicity against human MONO-MAC-6 cells incubated for 24 hrs by AlamarBlue staining based analysis
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[PMID: 36857518] |
| NCI-H838 | IC50 |
11 μM
Compound: 2
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Cytotoxicity against human NCI-H838 cells incubated for 24 hrs by AlamarBlue staining based analysis
Cytotoxicity against human NCI-H838 cells incubated for 24 hrs by AlamarBlue staining based analysis
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[PMID: 36857518] |
| PC-3 | IC50 |
5.7 μM
Compound: 2
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Cytotoxicity against human PC-3 cells incubated for 24 hrs by AlamarBlue staining based analysis
Cytotoxicity against human PC-3 cells incubated for 24 hrs by AlamarBlue staining based analysis
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[PMID: 36857518] |
| RPMI-8226 | IC50 |
0.17 μM
Compound: 2
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Cytotoxicity against human RPMI-8226 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay
Cytotoxicity against human RPMI-8226 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay
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[PMID: 36857518] |
| U-87MG ATCC | IC50 |
21.6 μM
Compound: 2
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Cytotoxicity against human U87 cells incubated for 24 hrs by AlamarBlue staining based analysis
Cytotoxicity against human U87 cells incubated for 24 hrs by AlamarBlue staining based analysis
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[PMID: 36857518] |
Justicidin B inhibits the growth of the pathogenic fungi Aspergillus fumigatus (MIC ≥ 1 μg/mL), Aspergillus flavus (MIC ≥ 12 μg/mL), and Candida albicans (MIC ≥ 4 μg/mL), but is not effective against Cryptococcus neoformans and Blastoschizomyces capitatus[1].
Justicidin B also exhibits strong activity against the trypomastigote form of Trypanosoma brucei rhodesiense (IC50 = 0.2 μg/mL) and moderate activity against Trypanosoma cruzi (IC500 = 2.6 μg/mL)[1].
Justicidin B shows cytotoxic activity and induction of apoptosis in MDA-MB-231 and MCF-7 breast cancer derived cell lines. The 24 h treatment of both cell lines increased the level of apoptotic DNA fragmentation. Exposure of MDA-MB-231 cells with Justicidin B leads to concentration dependent decrease in the expression of NFkB; whereas the treatment of MCF-7, is consistent with strong increase in the expression of this transcription factor[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 17951-19-8
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Appearance Solid
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Molecular Weight 364.35
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Formula C21H16O6
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Color White to off-white
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SMILES
O=C1OCC2=CC3=CC(OC)=C(OC)C=C3C(C4=CC=C(OCO5)C5=C4)=C12
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 12.5 mg/mL (34.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (3.43 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Jürg Gertsch, et al. Antifungal, antiprotozoal, cytotoxic and piscicidal properties of Justicidin B and a new arylnaphthalide lignan from Phyllanthus piscatorum. Planta Med. 2003 May;69(5):420-4. [Content Brief]
[2]. G Momekov, et al. Effect of justicidin B - a potent cytotoxic and pro-apoptotic arylnaphtalene lignan on human breast cancer-derived cell lines. Neoplasma. 2011;58(4):320-5. [Content Brief]
[3]. Iliana Ionkova, et al. Linum narbonense: A new valuable tool for biotechnological production of a potent anticancer lignan Justicidine B. Pharmacogn Mag. 2013 Jan;9(33):39-44. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7446 mL | 13.7231 mL | 27.4461 mL | 68.6153 mL |
| 5 mM | 0.5489 mL | 2.7446 mL | 5.4892 mL | 13.7231 mL | |
| 10 mM | 0.2745 mL | 1.3723 mL | 2.7446 mL | 6.8615 mL | |
| 15 mM | 0.1830 mL | 0.9149 mL | 1.8297 mL | 4.5744 mL | |
| 20 mM | 0.1372 mL | 0.6862 mL | 1.3723 mL | 3.4308 mL | |
| 25 mM | 0.1098 mL | 0.5489 mL | 1.0978 mL | 2.7446 mL | |
| 30 mM | 0.0915 mL | 0.4574 mL | 0.9149 mL | 2.2872 mL |
Justicidin B Related Classifications
HY-114275 Related Classifications
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