Cat. No.: HY-134266

8-Bromo-AMP

Name: 8-Bromo-AMP
Brand: MedChemExpress
SKU: HY-134266
Price: 250.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: 8-Bromoadenosine 5'-monophosphate; 8-Bromoadenylic acid)

1 Cited Publications

고객리뷰

Based on 1 publication(s) in Google Scholar

8-Bromo-AMP (8-Bromoadenosine 5'-monophosphate) is an AMP analog. 8-Bromo-AMP inhibits ADP-dependent glucokinase (ADPGK). 8-Bromo-AMP competitively inhibits ADPGK by binding to the nucleotide-binding site, inducing conformational changes, and acting on catalytic residues. 8-Bromo-AMP non-competitively inhibits rat adenylate kinase isozymes AK-M, AK II, and AK III. 8-Bromo-AMP inhibits T cell activation-induced ROS production and ROS-dependent IL-2 and IκB expression. 8-Bromo-AMP is used in research on cancer and myocardial ischemia-reperfusion injury.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity : 98.78%
CAS No.: 23567-96-6
화학식: C₁₀H₁₃BrN₅O₇P
분자량:426.12

보관:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

8-Bromo-AMP Chemical Structure

CAS No.: 23567-96-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

HY-12306A
8-Bromo-cAMP

PKA Activator

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (1)
용액&용해도
- In Vitro
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

IC₅₀ & Target

^[1]

ADPGK

IL-2

IκB

All AMPK Isoforms :

All Interleukin Related Isoforms :

All IKK Isoforms :

In Vitro

8-Bromo-AMP (8-Br-AMP) (1 mM) is a competitive inhibitor of hADPGK with a K_i of 270 μM^[1].
8-Bromo-AMP is a competitive inhibitor of phADPGK with a K_i of 160 μM^[1].
8-Bromo-AMP binds to wild-type phADPGK, phADPGK F272A, and hADPGK with K_d values of 16.85 μM, 17.44 μM, and 16.55 μM, respectively^[1].
8-Bromo-AMP (20 h) enhances β-adrenoceptor acquisition in primary rat hepatocyte cultures with an EC₅₀ of 47 μM^[3].
8-Bromo-AMP (30°C) competitively inhibits wheat seedling leaf 5'(3')-ribonucleotide phosphohydrolase with an apparent K_i of 9.1 μM^[4].
8-Bromo-AMP (24 h) almost completely inhibits PB-induced PBREM transactivation in the mouse liver^[6].
8-Bromo-AMP significantly attenuates PB-induced Cyp2b10 gene expression in the mouse liver^[6].
8-Bromo-AMP (8-Br-AMP) (10-100 μM) stimulates tracheary-element differentiation in Zinnia elegans mesophyll cell cultures in the absence of inductive concentrations of cytokinin^[9].
8-Bromo-AMP (25-100 μM; 30 min) dose-dependently reduces PMA-triggered ROS generation in Jurkat T cells, with 100 μM decreasing ROS to approximately half of the initial value^[1].
8-Bromo-AMP (25-100 μM; 30 min) decreases PMA (Phorbol 12-myristate 13-acetate) (HY-18739)/Ionomycin (HY-13434)-induced expression of IL-2 and IκB in Jurkat T cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:

Jurkat T cells
Concentration:

25 μM; 50 μM; 100 μM
Incubation Time:

30 min
Result:

Reduced the expression of IL-2 and IκB in PMA/ionomycin-induced Jurkat T cells.

In Vivo

8-Bromo-AMP (20 mg/kg; i.p.; single injection) inhibits Phenobarbital-induced PBREM transactivation and Cyp2b gene expression in mouse liver in vivo, confirming that AMPK is required for Phenobarbital-induced CAR activation^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male
C3H/HeN mice (7 weeks of age)
^[6]
Dosage:

20 mg/kg
Administration:

i.p.; single injection
Result:

Almost completely inhibited the PB-induced increase in PBREM reporter gene activity.
Significantly attenuated the induction of Cyp2b gene expression by PB.

Related Protocols :

Chemical Information

CAS No. 23567-96-6
Appearance Solid
분자량 426.12
화학식 C₁₀H₁₃BrN₅O₇P
Color White to off-white
SMILES

O=P(O)(OC[C@@H]1[C@H]([C@H]([C@H](N2C(Br)=NC3=C(N=CN=C32)N)O1)O)O)O
Synonyms

8-Bromoadenosine 5'-monophosphate; 8-Bromoadenylic acid
선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Mil Med Res

ADP-dependent glucokinase controls metabolic fitness in prostate cancer progression. [Abstract]

2023 Dec 12;10(1):64. PMID: 38082365

용액&용해도

In Vitro:

H₂O : 125 mg/mL (293.34 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3468 mL

11.7338 mL

23.4676 mL

5 mM

0.4694 mL

2.3468 mL

4.6935 mL

10 mM

0.2347 mL

1.1734 mL

2.3468 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (300 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Grudnik P, et al. Structural basis for ADP-dependent glucokinase inhibition by 8-bromo-substituted adenosine nucleotide. The Journal of biological chemistry. 2018 Jul 13;293(28):11088-11099.

[2]. Lesiak K, et al. Synthesis and biological activities of oligo(8-bromoadenylates) as analogues of 5'-O-triphosphoadenylyl(2'----5')adenylyl(2'----5')adenosine. Journal of medicinal chemistry. 1986 Jun;29(6):1015-22.

[3]. Sandnes D, et al. 8-bromo-cAMP and 8-CPT-cAMP increase the density of beta-adrenoceptors in hepatocytes by a mechanism not mimicking the effect of cAMP. Pharmacology & toxicology. 1996 Jul;79(1):15-22.

[4]. Polya GM, et al. Regulation of a plant 5'(3')-ribonucleotide phosphohydrolase by cyclic nucleotides and pyrimidine, purine, and cytokinin ribosides. Proceedings of the National Academy of Sciences of the United States of America. 1974 Apr;71(4):1299-303.

[5]. Niles RM, et al. Differential growth inhibition in two human carcinoma cell lines by cyclic adenosine 5'-monophosphate analogs. Journal of the National Cancer Institute. 1979 Oct;63(4):909-11.

[6]. Shindo S, et al. A physiological role of AMP-activated protein kinase in phenobarbital-mediated constitutive androstane receptor activation and CYP2B induction. The Biochemical journal. 2007 Feb 01;401(3):735-41.

[7]. Hai TT, et al. Species- or isozyme-specific enzyme inhibitors. 7. Selective effects in inhibitions of rat adenylate kinase isozymes by adenosine 5'-phosphate derivatives. Journal of medicinal chemistry. 1982 Jul;25(7):806-12.

[8]. Shah MM, et al. The connexin43 gap junction protein is phosphorylated by protein kinase A and protein kinase C: in vivo and in vitro studies. Molecular and cellular biochemistry. 2002 Sep;238(1-2):57-68.

[9]. Roberts AW, et al. Methylxanthines reversibly inhibit tracheary-element differentiation in suspension cultures of Zinnia elegans L. Planta. 1992 Mar;186(4):586-92.

[10]. Minelli A, et al. Activity of IMP- and AMP-preferring isoforms of 5'-nucleotidase from human seminal plasma with AMP analogues. Molecular genetics and metabolism. 1999 Jan;66(1):49-55.

[11]. Musi N, et al. AMP-activated protein kinase activity and glucose uptake in rat skeletal muscle. Am J Physiol Endocrinol Metab. 2001 May;280(5):E677-84.

[12]. Nakai T, et al. Effects of adenine nucleotide analogues on myocardial dysfunction during reperfusion after ischemia in dogs. Journal of cardiovascular pharmacology. 1996 Aug;28(2):264-70. [Content Brief]

[13]. Katsuki S, et al. Regulation of adenosine cyclic 3',5'-monophosphate and guanosine cyclic 3',5'-monophosphate levels and contractility in bovine tracheal smooth muscle. Mol Pharmacol. 1977 Mar;13(2):330-41.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	2.3468 mL	11.7338 mL	23.4676 mL	58.6689 mL
	5 mM	0.4694 mL	2.3468 mL	4.6935 mL	11.7338 mL
	10 mM	0.2347 mL	1.1734 mL	2.3468 mL	5.8669 mL
	15 mM	0.1565 mL	0.7823 mL	1.5645 mL	3.9113 mL
	20 mM	0.1173 mL	0.5867 mL	1.1734 mL	2.9334 mL
	25 mM	0.0939 mL	0.4694 mL	0.9387 mL	2.3468 mL
	30 mM	0.0782 mL	0.3911 mL	0.7823 mL	1.9556 mL
	40 mM	0.0587 mL	0.2933 mL	0.5867 mL	1.4667 mL
	50 mM	0.0469 mL	0.2347 mL	0.4694 mL	1.1734 mL
	60 mM	0.0391 mL	0.1956 mL	0.3911 mL	0.9778 mL
	80 mM	0.0293 mL	0.1467 mL	0.2933 mL	0.7334 mL
	100 mM	0.0235 mL	0.1173 mL	0.2347 mL	0.5867 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Bioz

8-Bromo-AMP

1 Cited Publications

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8-Bromo-AMP

Publications Citing Use of MedChemExpress (MCE) 8-Bromo-AMP

All AMPK Isoforms

All Interleukin Related Isoforms

All IKK Isoforms