Cat. No.: HY-80012

SJB3-019A

13 Cited Publications

고객리뷰

Based on 13 publication(s) in Google Scholar

SJB3-019A is a potent and novel USP1 inhibitor, 5 times more potent than SJB2-043 in promoting ID1 degradation and cytoxicity in K562 cells with IC₅₀ of 0.0781 μM.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity: 99.88%
CAS No.: 2070015-29-9
화학식: C₁₆H₈N₂O₃
분자량:276.25

보관:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

SJB3-019A Chemical Structure

CAS No.: 2070015-29-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (13)
용액&용해도
- In Vitro
- In Vivo
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

SJB3-019A is a potent and novel USP1 inhibitor, 5 times more potent than SJB2-043 in promoting ID1 degradation and cytoxicity in K562 cells with IC₅₀ of 0.0781 μM.

IC₅₀ & Target

IC50: 78.1 nM (ID1)

All Deubiquitinase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	662 nM Compound: SJB3-019A	Antiproliferative activity against human A549 cells harboring KRAS mutant incubated for 72 hrs by MTT assay Antiproliferative activity against human A549 cells harboring KRAS mutant incubated for 72 hrs by MTT assay	[PMID: 36221183]
EBC-1	IC₅₀	122 nM Compound: SJB3-019A	Antiproliferative activity against human EBC-1 cells harboring wild type KRAS incubated for 72 hrs by MTT assay Antiproliferative activity against human EBC-1 cells harboring wild type KRAS incubated for 72 hrs by MTT assay	[PMID: 36221183]
NCI-H1703	IC₅₀	93 nM Compound: SJB3-019A	Antiproliferative activity against human NCI-H1703 cells harboring wild type KRAS incubated for 72 hrs by MTT assay Antiproliferative activity against human NCI-H1703 cells harboring wild type KRAS incubated for 72 hrs by MTT assay	[PMID: 36221183]
NCI-H1975	IC₅₀	98 nM Compound: SJB3-019A	Antiproliferative activity against human NCI-H1975 cells harboring wild type KRAS incubated for 72 hrs by MTT assay Antiproliferative activity against human NCI-H1975 cells harboring wild type KRAS incubated for 72 hrs by MTT assay	[PMID: 36221183]
NCI-H23	IC₅₀	281 nM Compound: SJB3-019A	Antiproliferative activity against human NCI-H23 cells harboring KRAS mutant incubated for 72 hrs by MTT assay Antiproliferative activity against human NCI-H23 cells harboring KRAS mutant incubated for 72 hrs by MTT assay	[PMID: 36221183]

In Vitro

SJB3-019A (IC₅₀=0.0781 μM) is 5 times more potent than SJB2-043 in promoting ID1 degradation and cytoxicity in K562 cells. SJB3-019A increases the levels of Ub-FANCD2 and Ub-PCNA, and decreases the HR activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 2070015-29-9
Appearance Solid
분자량 276.25
화학식 C₁₆H₈N₂O₃
Color Light yellow to yellow
SMILES

O=C1C2=C(OC(C3=CC=CN=C3)=N2)C(C4=CC=CC=C41)=O
선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (13)

All

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Journal Impact Factor

Blood (2016) 128 (22): 3290
Blood

2015, 126 (23): 1813.
Mol Cell

Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases m6A Methylation. [Abstract]

2020 Nov 19;80(4):633-647.e7. PMID: 33217317
Sci Adv

USP1 deubiquitinates PARP1 to regulate its trapping and PARylation activity. [Abstract]

2024 Nov 15;10(46):eadp6567. PMID: 39536107
Sci Adv

USP1 links platinum resistance to cancer cell dissemination by regulating Snail stability. [Abstract]

2019 May 8;5(5):eaav3235. PMID: 31086816
Blood Cancer J

RSK1 dependency in FLT3-ITD acute myeloid leukemia. [Abstract]

2024 Nov 26;14(1):207. PMID: 39592591
Cell Chem Biol

Development of potent and selective inhibitors targeting the papain-like protease of SARS-CoV-2. [Abstract]

2021 Jun 17;28(6):855-865.e9. PMID: 33979649
J Med Chem

Discovery of Potent OTUB1/USP8 Dual Inhibitors Targeting Proteostasis in Non-Small-Cell Lung Cancer. [Abstract]

2022 Oct 27;65(20):13645-13659. PMID: 36221183
Eur J Pharm Biopharm

Inhibition of USP1 ameliorates hypertensive nephropathy through regulating oxidative stress and ferroptosis: a precise treatment via SJB3-019A nanodelivery. [Abstract]

2023 Dec:193:187-197. PMID: 37949326
Biomedicines

A Human DUB Protein Array for Clarification of Linkage Specificity of Polyubiquitin Chain and Application to Evaluation of Its Inhibitors. [Abstract]

2020 Jun 4;8(6):152. PMID: 32512835
J Biol Chem

USP1 promotes pancreatic cancer progression and autophagy by deubiquitinating ATG14. [Abstract]

2025 Mar;301(3):108190. PMID: 39814232
Int J Med Sci

Inhibition of USP1 induces apoptosis via ID1/AKT pathway in B-cell acute lymphoblastic leukemia cells. [Abstract]

2021 Jan 1;18(1):245-255. PMID: 33390793
University of Washington

2025.

용액&용해도

In Vitro:

DMSO : 10 mg/mL (36.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.6199 mL

18.0995 mL

36.1991 mL

5 mM

0.7240 mL

3.6199 mL

7.2398 mL

10 mM

0.3620 mL

1.8100 mL

3.6199 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1 mg/mL (3.62 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 15% Cremophor EL 85% Saline

Solubility: 10 mg/mL (36.20 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Helena Mistry, et al. Small molecule inhibitors of USP1 target ID1 degradation in leukemic cells. Mol Cancer Ther Published OnlineFirst October 15, 2013. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.6199 mL	18.0995 mL	36.1991 mL	90.4977 mL
	5 mM	0.7240 mL	3.6199 mL	7.2398 mL	18.0995 mL
	10 mM	0.3620 mL	1.8100 mL	3.6199 mL	9.0498 mL
	15 mM	0.2413 mL	1.2066 mL	2.4133 mL	6.0332 mL
	20 mM	0.1810 mL	0.9050 mL	1.8100 mL	4.5249 mL
	25 mM	0.1448 mL	0.7240 mL	1.4480 mL	3.6199 mL
	30 mM	0.1207 mL	0.6033 mL	1.2066 mL	3.0166 mL

Keywords

SJB3-019A

13 Cited Publications

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SJB3-019A

Ubiquitination Compound Library

Metabolic Enzyme Compound Library

Protease Inhibitor Library

Bioactive Compound Library Max

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Publications Citing Use of MedChemExpress (MCE) SJB3-019A

All Deubiquitinase Isoforms

Biological Activity

Chemical Information

Publications (13)

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

SJB3-019A

Ubiquitination Compound Library

Metabolic Enzyme Compound Library

Protease Inhibitor Library

Bioactive Compound Library Max

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Publications Citing Use of MedChemExpress (MCE) SJB3-019A

All Deubiquitinase Isoforms

Biological Activity

Chemical Information

Publications (13)

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

SJB3-019A Related Classifications

HY-80012 Related Classifications