HJC0152
Based on 3 publication(s) in Google Scholar
HJC0152 is a signal transducers and activators of transcription 3 (STAT3) inhibitor.
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- Purity: 99.75%
- CAS No.: 1420290-99-8
- 화학식: C15H14Cl3N3O4
- 분자량:406.65
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보관:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) HJC0152
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Customer Validation & Images
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WB
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IF
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RT-PCR
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In Vivo Efficacy Study
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Histological Imaging/Staining
Biological Activity
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STAT3 |
HJC0152 (compound 11) significantly inhibits cell proliferation and induces apoptosis accompanying cellular morphological changes at concentrations of 1, 5, and 10 μM. Results show that treatment with 10 μM HJC0152 decreases the STAT3 promoter activity in MDA-MB-231 cells by approximately 32%, and increasing the dose of HJC0152 to 20 μM further decreases STAT3 promoter activity by 62% as compare with control. Total STAT3 is reduced after treatment with HJC0152. HJC0152 induces cleaved caspase-3 and down regulates cyclin D1 in MDA-MB-231 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1420290-99-8
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Appearance Solid
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분자량 406.65
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화학식 C15H14Cl3N3O4
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Color White to yellow
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SMILES
O=C(NC1=CC=C([N+]([O-])=O)C=C1Cl)C2=CC(Cl)=CC=C2OCCN.[H]Cl
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (3)
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Journal Impact Factor
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Most Recent
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Int J Mol Sci
STAT3 Increases CVB3 Replication and Acute Pancreatitis and Myocarditis Pathology via Impeding Nuclear Translocation of STAT1 and Interferon-Stimulated Gene Expression. [Abstract]2024 Aug 19;25(16):9007. PMID: 39201692
HJC0152 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Aug 19;25(16):9007. [Abstract]
Nucleic or cytoplasmic p-STAT1 and STAT1 expressions were analyzed by immunoblotting analysis treated with HJC0152 (10 μM) for 48 h.
HJC0152 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Aug 19;25(16):9007. [Abstract]
IF assay to analyze nuclear translocation of p-STAT1 in STAT3 inhibitor-treated cells. Acinar 266-6 cells were mock-infected or infected with CVB3 and fixed 12 h p.i. The p-STAT1 expression and localizations were analyzed by confocal microscopy treated with HJC0152 (10 μM) for 48 h.
HJC0152 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Aug 19;25(16):9007. [Abstract]
mRNA levels of ISGs (OAS, MXA, ISG15, ISG56, Viperin and CXCL10 and GAPDH) from HJC0152 (10 μM)-treated cells were analyzed by RT-QPCR.
HJC0152 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Aug 19;25(16):9007. [Abstract]
Survival curve and weight loss curve in STAT3 inhibitor (HJC0152) (12.5 mg/kg, i.p.)-treated mice were followed by 7 dpi.
HJC0152 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Aug 19;25(16):9007. [Abstract]
Representative hematoxylin–eosin (H&E)-stained pancreas and heart sections from infected mice treated with HJC0152 (12.5 mg/kg, i.p.).
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Fish Shellfish Immunol
Butyrate induces STAT3/HIF-1α/IL-22 signaling via GPCR and HDAC3 inhibition to activate autophagy in head kidney macrophages from turbot (Scophthalmus maximus L.). [Abstract]2023 Dec:143:109214. PMID: 37977544 -
Naunyn Schmiedebergs Arch Pharmacol
Corynoline promotes apoptosis and inhibits proliferation of glioblastoma via regulating the STAT3/Bcl-2 signalling pathway. [Abstract]2025 Dec 11. PMID: 41379317
용액&용해도
DMSO : 20 mg/mL (49.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (4.92 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2 mg/mL (4.92 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 10 mg/mL (24.59 mM); Suspended solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Breast cancer MDA-MB-231 cells are incubated in 6-well plates (2.5×105/well). Cells are then treated with DMSO, or HJC0152 hydrochloride (compound 11) at different concentrations for 48 h, and then both adherent and floating cells are collected, washed once with PBS. Resuspended cells are incubated with 100 μL PBS containing 1% BSA and 100 μL Annexin V and dead cell detection reagent at room temperature for 20 min. Apoptosis is measured immediately using the Muse Cell Analyzer with the MuseTM Apoptosis Kit[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Fifty-four female nude mice are are used for orthotopic tumor studies at 4 to 6 weeks of age. The mice are maintained in a barrier unit with 12 h light-dark switch. Freshly harvested MDA-MB-231 cells (2.5×106 cells per mouse, resuspended in 100 μL PBS) are injected into the 3rd mammary fat pad of the mice, and then randomly assigned into 8 groups (5 to10 mice per group). For the intraperitoneal treatment experiment, the mice are treated daily with 2.5 mg/kg HJC0152 hydrochloride (compound 11) (Group A) or vehicle (Group D) when the tumor volume reaches 200 mm3. A Body weights and tumors volume are measured daily and tumor volume is calculated according to the formula V=0.5×L×W2, where L=length (mm) and W=width (mm)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
순도&문서
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4591 mL | 12.2956 mL | 24.5912 mL | 61.4779 mL |
| 5 mM | 0.4918 mL | 2.4591 mL | 4.9182 mL | 12.2956 mL | |
| 10 mM | 0.2459 mL | 1.2296 mL | 2.4591 mL | 6.1478 mL | |
| 15 mM | 0.1639 mL | 0.8197 mL | 1.6394 mL | 4.0985 mL | |
| 20 mM | 0.1230 mL | 0.6148 mL | 1.2296 mL | 3.0739 mL | |
| 25 mM | 0.0984 mL | 0.4918 mL | 0.9836 mL | 2.4591 mL | |
| 30 mM | 0.0820 mL | 0.4099 mL | 0.8197 mL | 2.0493 mL | |
| 40 mM | 0.0615 mL | 0.3074 mL | 0.6148 mL | 1.5369 mL |
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