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Cat. No.: HY-165607S

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MCB-22-174

Name: MCB-22-174
Brand: MedChemExpress
SKU: HY-165607S
Price: 270.00 USD
Availability: InStock
Rating: 5 (1 reviews)

고객리뷰

Based on 1 Customer Validation

MCB-22-174 is a deuterated Piezo1 agonist, with an EC₅₀ value of 6.28 μM. MCB-22-174 remarkably activates the CaMKII/ERK signaling pathway and initiates Ca²⁺ influx in rMSCs. MCB-22-174 significantly decreases the expression of chondrogenesis markers (Comp, Acan) and adipogenesis markers (Lpl, Fabp4) in MSCs. MCB-22-174 can effectively improve bone quality in hind-limb unloading (HU) model rats. MCB-22-174 can be used for the study of disuse osteoporosis (OP).

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity : 99.78%
CAS No.: 3058199-58-6
화학식: C₁₆H₁₄DCl₂N₅OS₂
분자량:429.37

보관:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

MCB-22-174 Chemical Structure

CAS No.: 3058199-58-6

사이즈

가격

재고

수량

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- In Vitro
- In Vivo
- 신청
Chemical Information
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

All ERK Isoforms :

All CaMK Isoforms :

In Vitro

MCB-22-174 (0-40 μM) exhibits potent agonistic activity on Piezo1 in C3H10T1/2 cells with an EC₅₀ value of 6.28 μM^[1].
MCB-22-174 (3-5 μM, 72 h) significantly upregulates the mRNA level of Runx2 in rMSCs and promotes osteogenesis more effectively than Yoda1 (HY-18723)^[1].
MCB-22-174 (5 μM, 5-15 min) remarkably upregulates p-ERK and p-CaMKII in rMSCs, and activates the CaMKII/ERK signaling pathway more efficiently than Yoda1^[1].
MCB-22-174 (5 μM) significantly decreases the expression of chondrogenesis markers (Comp, Acan) and adipogenesis markers (Lpl, Fabp4) in MSCs, showing a more significant suppression of chondrogenesis and adipogenesis than Yoda1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[1]

Cell Line:

rMSCs
Concentration:

3, 5 μM
Incubation Time:

72 h
Result:

Upregulated the mRNA level of Runx2 in rMSCs.

Western Blot Analysis^[1]

Cell Line:

rMSCs
Concentration:

5 μM
Incubation Time:

5, 10, 15 min
Result:

Upregulated p-ERK and p-CaMKII in rMSCs.

In Vivo

MCB-22-174 (2 mg/kg (5 μmol/kg), intraperitoneally injected on days 1, 4, 7, 10, and 13) can effectively improve bone quality in hind-limb unloading (HU) model rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

SD rats (2-month-old) were subjected to hind-limb unloading by tail suspension, with their tails fixed on the upper lids of cages to detach the hind limbs from the ground (forelimbs unaffected), simulating weightlessness to induce disuse osteoporosis^[1]
Dosage:

2 mg/kg (5 μmol/kg)
Administration:

Intraperitoneally injected on days 1, 4, 7, 10, and 13
Result:

Improved bone quality in HU model rats.
Upregulated bone volume ratio (BV/TV) and trabecular thickness (Tb.Th.).
Downregulated trabecular separation (Tb.Sp.).
Increased the fluorescent area of alkaline phosphatase (ALP).
Promoted osteogenesis, with better efficacy than Yoda1.

Related Protocols :

신청

1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.

Chemical Information

CAS No. 3058199-58-6
Unlabeled CAS 3087134-90-2
Appearance Solid
분자량 429.37
화학식 C₁₆H₁₄DCl₂N₅OS₂
Color Off-white to light yellow
SMILES

ClC(C=CC=C1Cl)=C1C([2H])SC2=NN=C(C3=NC=C(NC[C@H](O)C)N=C3)S2
선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hao R, et al. A Novel Piezo1 Agonist Promoting Mesenchymal Stem Cell Proliferation and Osteogenesis to Attenuate Disuse Osteoporosis. Small Sci. 2024 Jun 30;4(9):2400061. [Content Brief]

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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Biological Activity
- 제품 설명
- In Vitro
- In Vivo
- 신청
Chemical Information
순도&문서
References

상품명:
Cat. No.:
수량:
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상품명:
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MCB-22-174

All ERK Isoforms

All CaMK Isoforms