LY-266500
Based on 1 Customer Validation
LY-266500 is a succinyl CoA synthetase (SCS) specific inhibitor. LY-266500 has inhibitory effects on various parasites, such as Trypanosoma brucei brucei (IC50 =0.6 μM) and Leishmania donovani (IC50 =2.86 μM). LY-266500 can be used for research on parasitic infections.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.72%
- No. CAS: 147104-27-6
- Fòrmula: C9H5ClFNOS
- Peso molecular:229.66
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A2780 | GI50 |
>10 μM
Compound: 3j
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Cytotoxicity against human A2780 cells after 48 hrs by crystal violet staining
Cytotoxicity against human A2780 cells after 48 hrs by crystal violet staining
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[PMID: 19111471] |
| HEK293 | GI50 |
>10 μM
Compound: 3j
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Cytotoxicity against HEK293 cells after 48 hrs by crystal violet staining
Cytotoxicity against HEK293 cells after 48 hrs by crystal violet staining
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[PMID: 19111471] |
| HepG2 | GI50 |
>10 μM
Compound: 3j
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Cytotoxicity against human HepG2 cells after 48 hrs by crystal violet staining
Cytotoxicity against human HepG2 cells after 48 hrs by crystal violet staining
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[PMID: 19111471] |
| WiDr | GI50 |
>10 μM
Compound: 3j
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Cytotoxicity against human WiDr cells after 48 hrs by crystal violet staining
Cytotoxicity against human WiDr cells after 48 hrs by crystal violet staining
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[PMID: 19111471] |
Chemical Information
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No. CAS 147104-27-6
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Appearance Solid
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Peso molecular 229.66
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Fòrmula C9H5ClFNOS
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Color White to off-white
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SMILES
O=C1C=CSN1C2=CC=C(C(Cl)=C2)F
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (435.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.89 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.89 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.3543 mL | 21.7713 mL | 43.5426 mL | 108.8566 mL |
| 5 mM | 0.8709 mL | 4.3543 mL | 8.7085 mL | 21.7713 mL | |
| 10 mM | 0.4354 mL | 2.1771 mL | 4.3543 mL | 10.8857 mL | |
| 15 mM | 0.2903 mL | 1.4514 mL | 2.9028 mL | 7.2571 mL | |
| 20 mM | 0.2177 mL | 1.0886 mL | 2.1771 mL | 5.4428 mL | |
| 25 mM | 0.1742 mL | 0.8709 mL | 1.7417 mL | 4.3543 mL | |
| 30 mM | 0.1451 mL | 0.7257 mL | 1.4514 mL | 3.6286 mL | |
| 40 mM | 0.1089 mL | 0.5443 mL | 1.0886 mL | 2.7214 mL | |
| 50 mM | 0.0871 mL | 0.4354 mL | 0.8709 mL | 2.1771 mL | |
| 60 mM | 0.0726 mL | 0.3629 mL | 0.7257 mL | 1.8143 mL | |
| 80 mM | 0.0544 mL | 0.2721 mL | 0.5443 mL | 1.3607 mL | |
| 100 mM | 0.0435 mL | 0.2177 mL | 0.4354 mL | 1.0886 mL |
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