MeOSuc-AAPV-CMK
Based on 1 Customer Validation
MeOSuc-AAPV-CMK (Elastase Inhibitor III) is an elastase inhibitor that simultaneously inhibits Cathepsin G and Proteinase 3. MeOSuc-AAPV-CMK blocks the shedding of FcgRIIIb on the surface of human neutrophils. MeOSuc-AAPV-CMK reverses the elastase-mediated reduction in proinflammatory cytokine production by immune cells, partially restores IL-1β levels in immune cell co-culture systems, and prevents elastase-mediated adiponectin cleavage.
For research use only. We do not sell to patients.
- Purity: 99.45%
- CAS No.: 65144-34-5
- Formula: C22H35ClN4O7
- Molecular Weight:502.99
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Immunology/Inflammation Compound Library
HY-L007
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Metabolism/Protease Compound Library
HY-L012
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Differentiation Inducing Compound Library
HY-L038
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Angiogenesis-Related Compound Library
HY-L088
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Peptide Library
HY-L105
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Antidepressant Compound Library
HY-L108
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Pain-Related Compound Library
HY-L139
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Protease Inhibitor Library
HY-L147
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Autoimmune Disease Compound Library
HY-L156
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Cytokine Inhibitors Library
HY-L161
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Multi-Target Compound Library
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Radioprotector Library
HY-L178
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MCE Bioactive Compound Library
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Peptide Compound Library
HY-L105S
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
All Cathepsin Isoforms
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Biological Activity
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IL-1β |
MeOSuc-AAPV-CMK (200 μM; 10 min) inhibits Cytochalasin B (HY-16928) + N-Formyl-Met-Leu-Phe (HY-P0224)-induced FcγRIIIb shedding in human neutrophils with an inhibition rate of 70%, but exerts no effect on PMA-induced shedding[1].
MeOSuc-AAPV-CMK (10 μM; 24 h) can partially restore the production levels of IL-1β and TNF-α in human peripheral blood mononuclear cells (PBMCs) treated with neutrophil elastase (NE) and stimulated with heat-inactivated Candida albicans[2].
MeOSuc-AAPV-CMK (10 μM; 24 h) partially restores IL-1β production in human PBMCs stimulated with heat-inactivated Candida albicans and co-cultured with PMNs, but does not alter TNF-α levels[2].
MeOSuc-AAPV-CMK inhibits leukocyte elastase-mediated cleavage of purified mouse full-length adiponectin[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human PBMCs
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Concentration:10 μM
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Incubation Time:24 h
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Result:Partially restored the production levels of IL-1β and TNF-α in human peripheral blood mononuclear cells (PBMCs) treated with neutrophil elastase (NE) and stimulated with heat-inactivated Candida albicans.
Partially restored IL-1β production in human PBMCs stimulated with heat-inactivated Candida albicans and co-cultured with PMNs, but does not alter TNF-α levels.
Chemical Information
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CAS No. 65144-34-5
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Appearance Solid
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Molecular Weight 502.99
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Formula C22H35ClN4O7
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Color White to off-white
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Synonyms
Elastase Inhibitor III
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Sequence
{MeOSuc}-Ala-Ala-Pro-Val-{CMK}
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Sequence Shortening
{MeOSuc}-AAPV-{CMK}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (198.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9881 mL | 9.9406 mL | 19.8811 mL | 49.7028 mL |
| 5 mM | 0.3976 mL | 1.9881 mL | 3.9762 mL | 9.9406 mL | |
| 10 mM | 0.1988 mL | 0.9941 mL | 1.9881 mL | 4.9703 mL | |
| 15 mM | 0.1325 mL | 0.6627 mL | 1.3254 mL | 3.3135 mL | |
| 20 mM | 0.0994 mL | 0.4970 mL | 0.9941 mL | 2.4851 mL | |
| 25 mM | 0.0795 mL | 0.3976 mL | 0.7952 mL | 1.9881 mL | |
| 30 mM | 0.0663 mL | 0.3314 mL | 0.6627 mL | 1.6568 mL | |
| 40 mM | 0.0497 mL | 0.2485 mL | 0.4970 mL | 1.2426 mL | |
| 50 mM | 0.0398 mL | 0.1988 mL | 0.3976 mL | 0.9941 mL | |
| 60 mM | 0.0331 mL | 0.1657 mL | 0.3314 mL | 0.8284 mL | |
| 80 mM | 0.0249 mL | 0.1243 mL | 0.2485 mL | 0.6213 mL | |
| 100 mM | 0.0199 mL | 0.0994 mL | 0.1988 mL | 0.4970 mL |
MeOSuc-AAPV-CMK Related Classifications
HY-136888 Related Classifications
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