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MitoBloCK-10 

Cat. No.: HY-115467 Purity: 98.80%
Handling Instructions

MitoBloCK-10 (MB-10) is the first small molecule modulator to attenuate protein-associated motor (PAM) complex activity. MitoBloCK-10 (MB-10) inhibits Tim44 (C-terminal domain) binding to the precursor and to Hsp70.

For research use only. We do not sell to patients.

MitoBloCK-10 Chemical Structure

MitoBloCK-10 Chemical Structure

CAS No. : 394694-98-5

Size Price Stock Quantity
500 μg USD 50 In-stock
Estimated Time of Arrival: December 31
1 mg USD 80 In-stock
Estimated Time of Arrival: December 31
1 mg X 5 USD 200 In-stock
Estimated Time of Arrival: December 31

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Based on 1 publication(s) in Google Scholar

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Description

MitoBloCK-10 (MB-10) is the first small molecule modulator to attenuate protein-associated motor (PAM) complex activity. MitoBloCK-10 (MB-10) inhibits Tim44 (C-terminal domain) binding to the precursor and to Hsp70[1].

In Vitro

MB-10 (100 μM) is a potential attenuator of protein import into mitochondria[1].
MB-10 inhibits the import of substrates that use the TIM23 import pathway[1].
MB-10 (0-100 μM) inhibits protein import into mammalian mitochondria[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: A plasmid expressing Su9-Ura3-myc was integrated at the LEU2 locus in WT and tim23-2 strains.
Concentration: 100 μM.
Incubation Time: 30 min.
Result: WT strain expressing Su9-Ura3 failed to grow, whereas the tim23-2 mutant expressing Su9-Ura3 grew much faster. The strains grew at a similar rate when the medium was supplemented with uracil.

Cell Viability Assay[1]

Cell Line: HeLa cells.
Concentration: 0-100 μM.
Incubation Time: 24 h.
Result: Inhibited HeLa cells vibility, with an IC50 of 17.2 μM.
Molecular Weight

293.27

Formula

C₁₂H₈FN₃O₃S

CAS No.
SMILES

O=C(N/N=C/C1=CC=C([N+]([O-])=O)S1)C2=CC=CC(F)=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (426.23 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4098 mL 17.0491 mL 34.0983 mL
5 mM 0.6820 mL 3.4098 mL 6.8197 mL
10 mM 0.3410 mL 1.7049 mL 3.4098 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.08 mg/mL (7.09 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are provided by MCE.
References
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Keywords:

MitoBloCK-10MitoBloCK10MitoBloCK 10OthersMitochondrialProteinImportTim44Inhibitorinhibitorinhibit

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Product Name:
MitoBloCK-10
Cat. No.:
HY-115467
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