MitoBloCK-10
Based on 3 publication(s) in Google Scholar
MitoBloCK-10 (MB-10) is the first small molecule modulator to attenuate protein-associated motor (PAM) complex activity. MitoBloCK-10 (MB-10) inhibits Tim44 (C-terminal domain) binding to the precursor and to Hsp70.
For research use only. We do not sell to patients.
- Purity: 98.99%
- CAS No.: 394694-98-5
- Formula: C12H8FN3O3S
- Molecular Weight:293.27
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) MitoBloCK-10
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Biological Activity
MB-10 (100 μM) is a potential attenuator of protein import into mitochondria[1].
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MB-10 inhibits the import of substrates that use the TIM23 import pathway[1].
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MB-10 (0-100 μM) inhibits protein import into mammalian mitochondria[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A plasmid expressing Su9-Ura3-myc was integrated at the LEU2 locus in WT and tim23-2 strains.
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Concentration:100 μM.
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Incubation Time:30 min.
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Result:WT strain expressing Su9-Ura3 failed to grow, whereas the tim23-2 mutant expressing Su9-Ura3 grew much faster. The strains grew at a similar rate when the medium was supplemented with uracil.
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Cell Line:HeLa cells.
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Concentration:0-100 μM.
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Incubation Time:24 h.
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Result:Inhibited HeLa cells vibility, with an IC50 of 17.2 μM.
Chemical Information
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CAS No. 394694-98-5
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Appearance Solid
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Molecular Weight 293.27
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Formula C12H8FN3O3S
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Color Light yellow to yellow
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SMILES
O=C(N/N=C/C1=CC=C([N+]([O-])=O)S1)C2=CC=CC(F)=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (3)
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Journal Impact Factor
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Most Recent
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Commun Biol
Cytosolic retention of HtrA2 during mitochondrial protein import stress triggers the DELE1-HRI pathway. [Abstract]2024 Mar 30;7(1):391. PMID: 38555279 -
Am J Physiol Cell Physiol
Divergent mitochondrial stressors elicit specific retrograde signaling pathways in muscle myotubes. [Abstract]2026 Jun 1;330(6):C1721-C1736. PMID: 42126081 -
Solvent & Solubility
DMSO : 125 mg/mL (426.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (7.09 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4098 mL | 17.0491 mL | 34.0983 mL | 85.2457 mL |
| 5 mM | 0.6820 mL | 3.4098 mL | 6.8197 mL | 17.0491 mL | |
| 10 mM | 0.3410 mL | 1.7049 mL | 3.4098 mL | 8.5246 mL | |
| 15 mM | 0.2273 mL | 1.1366 mL | 2.2732 mL | 5.6830 mL | |
| 20 mM | 0.1705 mL | 0.8525 mL | 1.7049 mL | 4.2623 mL | |
| 25 mM | 0.1364 mL | 0.6820 mL | 1.3639 mL | 3.4098 mL | |
| 30 mM | 0.1137 mL | 0.5683 mL | 1.1366 mL | 2.8415 mL | |
| 40 mM | 0.0852 mL | 0.4262 mL | 0.8525 mL | 2.1311 mL | |
| 50 mM | 0.0682 mL | 0.3410 mL | 0.6820 mL | 1.7049 mL | |
| 60 mM | 0.0568 mL | 0.2842 mL | 0.5683 mL | 1.4208 mL | |
| 80 mM | 0.0426 mL | 0.2131 mL | 0.4262 mL | 1.0656 mL | |
| 100 mM | 0.0341 mL | 0.1705 mL | 0.3410 mL | 0.8525 mL |
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