Cat. No.: HY-134818

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ML-SI1

7 Cited Publications

Customer Review

Based on 7 publication(s) in Google Scholar

ML-SI1, a racemic mixture of diastereomers, is a TRPML inhibitor with an IC₅₀ value of 15 μM for TRPML1.

For research use only. We do not sell to patients.

Purity: 99.80%
Formula: C₂₃H₂₆Cl₂N₂O₃
Molecular Weight:449.37

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

ML-SI1 Chemical Structure

Size

Price

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Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (7)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

ML-SI1, a racemic mixture of diastereomers, is a TRPML inhibitor with an IC₅₀ value of 15 μM for TRPML1^[1].

IC₅₀ & Target

IC50: 15 μM (TRPML1)^[1]

All TRP Channel Isoforms :

In Vitro

ML-SI1 is an inseparable racemic mixture of cis-/trans-isomers (55:45) in a short synthetic sequence and its inhibitory activity on hTRPML1 (and a weak effect on TRPML2) after activation with ML-SA1. Fura-2 based single cell calcium imaging experiments confirmed that the synthesized racemic ML-SI1 (10 μM) has an inhibitory effect on hTRPML1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Appearance Solid
Molecular Weight 449.37
Formula C₂₃H₂₆Cl₂N₂O₃
Color Off-white to yellow
SMILES

O=C(N1C2=CC=C(C=C2C(C1C)CCN3CCOCC3)OC)C4=C(Cl)C(Cl)=CC=C4.[Mixture of diastereomers]
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (7)

All IFCell Imaging/StainingBio/Physico-chemical AssayWB

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Journal Impact Factor

Burns Trauma

Flufenamic acid inhibits pyroptosis in ischemic flaps via the AMPK-TRPML1-Calcineurin signaling pathway. [Abstract]

2025 Feb 17:13:tkaf007. PMID: 40655077

ML-SI1 purchased from MedChemExpress. Usage Cited in: Burns Trauma. 2025 Feb 17:13:tkaf007. [Abstract]

ML-SI1 (1 mg/kg; i.p.; 30 min) countered FFA's effects on TFE3 nuclear levels and activation of the signaling pathway in C57BL/6J mice.
Acta Pharmacol Sin

Corynoxine promotes TFEB/TFE3-mediated autophagy and alleviates Aβ pathology in Alzheimer's disease models. [Abstract]

2024 May;45(5):900-913. PMID: 38225393

ML-SI1 purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 May;45(5):900-913. [Abstract]

ML-SI1 (10 μM; 24 h) blocked Cory-induced TFEB/TFE3 nuclear translocation in N2a cells.

ML-SI1 purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 May;45(5):900-913. [Abstract]

ML-SI1 (10 μM; 24 h) blocked the increased LC3B-II and the decreased SQSTM1 in N2a cells.

ML-SI1 purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 May;45(5):900-913. [Abstract]

ML-SI1 blocked the transient and significant increase in Ca²⁺ concentration in N2a cells induced by cory in Ca²⁺-free buffer.
Ecotoxicol Environ Saf

Lysosomal iron accumulation and subsequent lysosomes-mitochondria iron transmission mediate PFOS-induced hepatocyte ferroptosis. [Abstract]

2024 Oct 1:284:116890. PMID: 39146593

ML-SI1 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2024 Oct 1:284:116890. [Abstract]

ML-SI1 (10 μM; 1 h) further heightened lysosomal iron levels in PFOS-exposed L-O2 cells.
Ecotoxicol Environ Saf

Blunting ROS/TRPML1 pathway protects AFB1-induced porcine intestinal epithelial cells apoptosis by restoring impaired autophagic flux. [Abstract]

2023 Jun 1:257:114942. PMID: 37086622

ML-SI1 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Jun 1:257:114942. [Abstract]

ML-SI1 (1.25 μM; 24 h) significantly reduces the AFB1-induced number of autophagosomes in IPEC-J2 cells.

ML-SI1 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Jun 1:257:114942. [Abstract]

ML-SI1 (1.25 μM; 24 h) significantly reduces the AFB1-induced expression of LC3, SQSTM1, Cleaved-Caspase3 and TRPML1 in IPEC-J2 cells.
Biochim Biophys Acta Mol Cell Res

Impaired autophagic flux and dedifferentiation in podocytes lacking Asah1 gene: Role of lysosomal TRPML1 channel. [Abstract]

2023 Jan;1870(1):119386. PMID: 36302466
Mol Biol Cell

Stress-induced microautophagy is coordinated with lysosome biogenesis and regulated by PIKfyve. [Abstract]

2024 May 1;35(5):ar70. PMID: 38536415
bioRxiv

The lysosomal cation channel TRPML1 regulates the oligodendrocyte cytoskeleton. [Abstract]

2026 Jun 18:2026.06.16.731945. PMID: 42367851

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (222.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2253 mL

11.1267 mL

22.2534 mL

5 mM

0.4451 mL

2.2253 mL

4.4507 mL

10 mM

0.2225 mL

1.1127 mL

2.2253 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Charlotte Leser, et al. Chemical and pharmacological characterization of the TRPML calcium channel blockers ML-SI1 and ML-SI3. Eur J Med Chem. 2021 Jan 15;210:112966. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2253 mL	11.1267 mL	22.2534 mL	55.6334 mL
	5 mM	0.4451 mL	2.2253 mL	4.4507 mL	11.1267 mL
	10 mM	0.2225 mL	1.1127 mL	2.2253 mL	5.5633 mL
	15 mM	0.1484 mL	0.7418 mL	1.4836 mL	3.7089 mL
	20 mM	0.1113 mL	0.5563 mL	1.1127 mL	2.7817 mL
	25 mM	0.0890 mL	0.4451 mL	0.8901 mL	2.2253 mL
	30 mM	0.0742 mL	0.3709 mL	0.7418 mL	1.8544 mL
	40 mM	0.0556 mL	0.2782 mL	0.5563 mL	1.3908 mL
	50 mM	0.0445 mL	0.2225 mL	0.4451 mL	1.1127 mL
	60 mM	0.0371 mL	0.1854 mL	0.3709 mL	0.9272 mL
	80 mM	0.0278 mL	0.1391 mL	0.2782 mL	0.6954 mL
	100 mM	0.0223 mL	0.1113 mL	0.2225 mL	0.5563 mL

Keywords

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ML-SI1

Membrane Transporter/Ion Channel Compound Library

Neuronal Signaling Compound Library

Mechanoreceptors Compound Library

Pain-Related Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Ion Channel Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) ML-SI1

All TRP Channel Isoforms

Biological Activity

Chemical Information

Publications (7)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

ML-SI1

Membrane Transporter/Ion Channel Compound Library

Neuronal Signaling Compound Library

Mechanoreceptors Compound Library

Pain-Related Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Ion Channel Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) ML-SI1

All TRP Channel Isoforms

Biological Activity

Chemical Information

Publications (7)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

ML-SI1 Related Classifications

HY-134818 Related Classifications