mTOR-IN-29
mTOR-IN-29 (Compound 4k) is an mTOR inhibitor with a IC50 of ~120 nM. mTOR-IN-29 inhibits mTOR kinase activity without affecting the phosphorylation of STAT3. mTOR-IN-29 acts as a cytotoxic agent against proliferating and senescent cells. mTOR-IN-29 can be used in studies related to glioblastoma.
For research use only. We do not sell to patients.
- Formula: C16H15N5S
- Molecular Weight:309.39
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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STAT3 |
mTOR-IN-29 (1-50 μM; 72 h) potently inhibits the growth of BJ, RPE-1 hTERT and U-251 MG cells, and its activity is almost equivalent to that of Torkinib (HY-10474)[1].
mTOR-IN-29 (100 μM; 15 min pre-incubation) does not inhibit IL-6-induced STAT3 phosphorylation (Y705) in RPE-1 hTERT cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:normal human dermal fibroblasts BJ, non-transformed telomerase-immortalized retinal pigment epithelial cells (RPE-1 hTERT), cancerous human glioblastoma cell line U-251 MG
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Concentration:1 μM, 10 μM, 50 μM
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Incubation Time:72 h
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Result:Suppressed growth of BJ, RPE-1 hTERT, and U-251 MG cells to a degree almost comparable to Torkinib.
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Cell Line:non-transformed telomerase-immortalized retinal pigment epithelial cells (RPE-1 hTERT), cancerous human glioblastoma cell lines U-251 MG and T98 G
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Concentration:0.05 μM, 0.1 μM, 0.5 μM, 1 μM, 5 μM, 10 μM, 50 μM
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Incubation Time:24 h
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Result:Showed growth suppression activity comparable to torkinib across all tested cell lines; T98 G cells appeared most sensitive (difference not statistically significant).
Exhibited crystal violet assay IC50 values of 6.5 μM (RPE-1 hTERT), 7.5 μM (U-251 MG).
Exhibited resazurin assay IC50 values of 7.7 μM (RPE-1 hTERT), 4.8 μM (U-251 MG).
Chemical Information
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Molecular Weight 309.39
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Formula C16H15N5S
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SMILES
NC1=C2C(N(C(C)C)N=C2C3=CC4=C(C=CC=C4)S3)=NC=N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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