2. PI3K/Akt/mTOR Stem Cell/Wnt JAK/STAT Signaling
  3. mTOR STAT
  4. mTOR-IN-29

mTOR-IN-29 (Compound 4k) is an mTOR inhibitor with a IC50 of ~120 nM. mTOR-IN-29 inhibits mTOR kinase activity without affecting the phosphorylation of STAT3. mTOR-IN-29 acts as a cytotoxic agent against proliferating and senescent cells. mTOR-IN-29 can be used in studies related to glioblastoma.

For research use only. We do not sell to patients.

mTOR-IN-29

mTOR-IN-29 Chemical Structure

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mTOR-IN-29 Related Antibodies

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mTOR mTORC1 mTORC2

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STAT3 STAT5 STAT6 STAT1

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Description

mTOR-IN-29 (Compound 4k) is an mTOR inhibitor with a IC50 of ~120 nM. mTOR-IN-29 inhibits mTOR kinase activity without affecting the phosphorylation of STAT3. mTOR-IN-29 acts as a cytotoxic agent against proliferating and senescent cells. mTOR-IN-29 can be used in studies related to glioblastoma[1].

IC50 & Target[1]

STAT3

 

In Vitro

mTOR-IN-29 (1-50 μM; 72 h) potently inhibits the growth of BJ, RPE-1 hTERT and U-251 MG cells, and its activity is almost equivalent to that of Torkinib (HY-10474)[1].
mTOR-IN-29 (100 μM; 15 min pre-incubation) does not inhibit IL-6-induced STAT3 phosphorylation (Y705) in RPE-1 hTERT cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

mTOR-IN-29 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: normal human dermal fibroblasts BJ, non-transformed telomerase-immortalized retinal pigment epithelial cells (RPE-1 hTERT), cancerous human glioblastoma cell line U-251 MG
Concentration: 1 μM, 10 μM, 50 μM
Incubation Time: 72 h
Result: Suppressed growth of BJ, RPE-1 hTERT, and U-251 MG cells to a degree almost comparable to Torkinib.

Cell Proliferation Assay[1]

Cell Line: non-transformed telomerase-immortalized retinal pigment epithelial cells (RPE-1 hTERT), cancerous human glioblastoma cell lines U-251 MG and T98 G
Concentration: 0.05 μM, 0.1 μM, 0.5 μM, 1 μM, 5 μM, 10 μM, 50 μM
Incubation Time: 24 h
Result: Showed growth suppression activity comparable to torkinib across all tested cell lines; T98 G cells appeared most sensitive (difference not statistically significant).
Exhibited crystal violet assay IC50 values of 6.5 μM (RPE-1 hTERT), 7.5 μM (U-251 MG).
Exhibited resazurin assay IC50 values of 7.7 μM (RPE-1 hTERT), 4.8 μM (U-251 MG).
Molecular Weight

309.39

Formula

C16H15N5S
SMILES

NC1=C2C(N(C(C)C)N=C2C3=CC4=C(C=CC=C4)S3)=NC=N1
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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mTOR-IN-29 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

mTOR-IN-29mTORSTATMammalian target of RapamycinU-251 MG cellsT98 G cellsBJ cellsmTOR pathwaymTOR kinaseSTAT3 phosphorylationglioblastomaIL-6RPE-1 hTERT cellsS6K phosphorylationInhibitorinhibitorinhibit

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