NLRP3-IN-32
NLRP3-IN-32 (compound 7a), a 3, 4-dihydronaphthalene-1(2H)-one derivative, is a potential NLRP3 inflammatory vesicles inhibitor. NLRP3-IN-32 can block the assembly and activation of NLRP3 inflammasome by down-regulating the expression of NLPR3 and apoptosis-associated speck-like protein containing a CARD (ASC), and inhibiting the production of reactive oxygen species (ROS) and other inflammatory mediators. NLRP3-IN-32 inhibits the phosphorylation of IκBα and NF-κB/p65 and the nuclear translocation of p65, thereby inhibiting NF-κB signaling.
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- 화학식: C21H22BrN3O
- 분자량:412.32
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
IC50 & Target
[1]|
NLRP3 inflammasome |
p65 |
In Vitro
NLRP3-IN-32 (compound 7a; 1.5, 3, 6 μM) can reverse the release of macrophage inflammatory factors TNF-α, IL-6, IL-18 and IL-1β under LPS (HY-D1056) stimulation in a dose-dependent manner in RAW246.7 cells[1].
NLRP3-IN-32 (1.5, 3, 6 μM) inhibits the activation of NLRP3 inflammatory vesicles by dose-dependently scavenging intracellular ROS and NO production by LPS[1].
NLRP3-IN-32 (1.5, 3, 6 μM) has low toxicity to RAW246.7 cells and has the apoptosis rates of 3.8%, 5.6%, and 6.8%, respectively[1].
NLRP3-IN-32 (1.5, 3, 6 μM; 24 h) shows the dose-dependent inhibition on the phosphorylation of IκBα and p65 phosphorylation levels stimulated with LPS (1.0 μg/mL; 2h) to inhibit NF-κB signaling pathway activation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
- Kinase activity and phosphorylation assays
- RNA extraction experimental
- Western Blot
- Cytoplasmic-Nuclear Fractionated Protein Extraction
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Annexin V plus membrane-impermeant dye apoptosis staining
- ROS/oxidative-stress fluorescent staining
- Apoptosis Solutions
- Protocol for Kinase activity and phosphorylation assays
- Research Protocol for Inflammation-related Diseases
- Pyroptosis Solutions
Chemical Information
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분자량 412.32
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화학식 C21H22BrN3O
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SMILES
O=C(C1=C(CC/2)C=CC(Br)=C1)C2=C\C3=NC=C(N4CCN(C)CC4)C=C3
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
순도&문서
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
NLRP3-IN-32 Related Classifications
HY-161329 Related Classifications
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