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Targeted therapy! The Capetin Prize winning "Click Chemistry" can be used like this!Targeted therapy! The Nobel Prize winning "Click Chemistry" can be used like this!2025-02-06
72 Results for "
in vitro transcription
" in MedChemExpress (MCE) Product Catalog:
Products (72)
72 Results for "in vitro transcription" in MCE Product Catalog:
12
12 Publications Verification
Research Areas:
IMT1 is a first-in-class specific and noncompetitive human mitochondrial RNA polymerase (POLRMT) inhibitor. IMT1 causes a conformational change of POLRMT, which blocks substrate binding and transcription in a dose-dependent way in vitro. IMT1 reduces deoxynucleoside triphosphate levels and citric acid cycle intermediates, resulting in a marked depletion of cellular amino acid levels. IMT1 has the potential for mitochondrial transcription disorders related diseases .
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8 Cited Publications
DB1976 dihydrochloride is a selenophene analog of DB270 and a potent and cell-permeable fully efficacious transcription factor PU.1 inhibitor. DB1976 dihydrochloride potently inhibits PU.1 binding (IC50 of 10 nM) and strongly inhibits the PU.1/DNA complex (with high DB1976-λB affinity, KD of 12 nM) in vitro. DB1976 dihydrochloride has apoptosis-inducing effect .
7
Peretinoin is an oral acyclic retinoid with a vitamin A-like structure that targets retinoid nuclear receptors such as retinoid X receptor (RXR) and retinoic acid receptor (RAR). Peretinoin reduces the mRNA level of sphingosine kinase 1 (SPHK1) in vitro by downregulating a transcription factor, Sp1 . Peretinoin prevents the progression of non-alcoholic steatohepatitis (NASH) and the development of hepatocellular carcinoma (HCC) through activating the autophagy pathway by increased Atg16L1 expression . Peretinoin inhibits HCV RNA amplification and virus release by altering lipid metabolism with a EC50 of 9 μM .
6
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Poly(deoxyadenylic-thymidylic) acid (Poly(dA:dT)) sodium is a double-stranded DNA stimulant. Poly(deoxyadenylic-thymidylic) acid sodium is recognized by the intracellular DNA sensor DDX41 and activates the innate immune pathway via the adaptor protein STING, inducing the production of cytokines such as type I interferons. Poly(deoxyadenylic-thymidylic) acid sodium also serves as an in vitro transcription template for free RNA polymerase .
5
5 Cited Publications
Cat. No.: HY-N0866
CAS No.: 54706-99-9
Category:
Terpenoids Euphorbia kansui T. N. Liou ex S. B. Ho Diterpenoids Plants Disease Research Fields Inflammation/Immunology Euphorbiaceae Extract
Target:
2
2 Cited Publications
Synonyms: 6-Methyladenosine (Standard); N-Methyladenosine (Standard)
1
1 Cited Publications
Synonyms: ac4CTP sodium solution (100mM)
Target:
Research Areas:
N4-Acetylcytidine triphosphate sodium solution (100mM) is efficiently used as a substrate in T7 Polymerase-catalyzed in vitro
transcription and it can be incorporated into multiple templates .
1
1 Cited Publications
Cat. No.: HY-N6069
CAS No.: 38963-94-9
Category:
Ketones, Aldehydes, Acids Rosaceae Extract Plants Rubus corchorifolius Linn. f. Disease Research Fields Inflammation/Immunology
Target:
1
1 Cited Publications
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JNJ-8003 is a potent and orally active non-nucleoside RSV polymerase inhibitor with an IC50 of 0.29 nM. JNJ-8003 targets the L protein polymerase complex of RSV (IC50 = 0.67 nM), and blocks the transcription and replication of the viral genome by inhibiting the activity of RNA-dependent RNA polymerase (RdRp). JNJ-8003 displays subnanomolar activity in vitro as well as prominent efficacy in mice and a neonatal lamb models. JNJ-8003 can be used for the study of respiratory syncytial virus (RSV) .
1
Research Areas:
5-Methylcytidine 5′-triphosphate trisodium (5-Methyl-CTP trisodium) is a modified cytidine triphosphate nucleotide. 5-Methylcytidine 5′-triphosphate trisodium serves as a modified nucleotide substrate for in vitro transcription, partially or completely replacing CTP. 5-Methylcytidine 5′-triphosphate trisodium enhances mRNA stability and translation efficiency, and reduces activation of the innate immune system. 5-Methylcytidine 5′-triphosphate trisodium is used in RNA synthesis-related research .
1
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5-Iodo-cytidine (5-Iodo-D-cytidine) is a purine nucleoside analog that acts as an intermediate in organic synthesis. 5-Iodo-cytidine serves as a chemically modified nucleotide to replace partial cytidine residues in in vitro transcription, enhancing the translation efficiency and stability of chemically modified mRNA. Purine nucleoside analogs exhibit broad anti-tumor activity and target indolent lymphoid malignancies. 5-Iodo-cytidine can be used in mRNA therapy and tumor-related research .
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Cat. No.: HY-174497
eGFP mRNA expresses green fluorescent protein derived from the jellyfish Aequorea victoria. eGFP is a commonly used direct-detection reporter gene in mammalian cell culture, which produces bright green fluorescence. This product is synthesized without modified nucleosides, with a Cap1 version cap analog at the 5' end and a 100nt PolyA tail at the 3' end. It can be used as a positive reference for IVT (in vitro transcription) or LNP delivery systems.
Cat. No.: HY-159098
Research Areas:
dWIZ-1 is an orally active molecular glue and chemical probe targeting the WIZ transcription factor, which based on an IMiD backbone, binding to human WIZ with an affinity of 3.5 μM. dWIZ-1 recruits WIZ to the cereblon-DDB1 complex via its ZF7 domain, thereby triggering proteasome-dependent degradation of WIZ. dWIZ-1 significantly induces fetal hemoglobin expression in erythroblasts while reducing the level of inhibitory H3K9 dimethylation at WIZ binding sites such as the β-globin locus. Meanwhile, dWIZ-1 does not affect the proliferation and differentiation of erythroblasts, and no cytotoxicity is observed in in vitro cells or cynomolgus monkey models. dWIZ-1 serves as a critical tool molecule for investigating the mechanism and underlying pathways of sickle cell disease .
Synonyms: m7GpppA diammonium; N7-Methyl-guanosine-5'-triphosphate-5'-adenosine diammonium
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m7G(5')ppp(5')A (m7GpppA) diammonium is a dinucleotide cap analog that can be used for in vitro RNA transcription .
Cat. No.: HY-125818I
CAS No.: 36051-68-0
Synonyms: Cytidine triphosphate disodium salt, 100 mM Solution, PCR Grade; 5'-CTP disodium salt, 100 mM Solution, PCR Grade
Cat. No.: HY-D1686
CAS No.: 186033-13-6
Synonyms: Biotin-16-UTP tetralithium
Cat. No.: HY-W013098
CAS No.: 74299-50-6
Synonyms: 5'-Dexoadenosine triphosphate disodium
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Research Areas:
T7 RNA polymerase is a polymerase expressed by Escherichia coli from the RNA polymerase gene of T7 bacteriophage. T7 RNA polymerase is highly specific and involved in in vitro transcription (IVT) of mRNA. In the presence of Mg 2+, T7 RNA polymerase only uses the single-stranded or double-stranded DNA containing the T7 promoter sequence as a template, and uses NTP as a substrate to synthesize RNA complementary to the single-stranded DNA downstream of the promoter .
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Protocols
Targeted therapy! The Capetin Prize winning "Click Chemistry" can be used like this!Targeted therapy! The Nobel Prize winning "Click Chemistry" can be used like this!A suitable model is crucial in drug screening experiments. Organs can mimic the three-dimensional functional structure of internal organs, have similar spatial organization to corresponding organs, maintain some key characteristics, and reproduce some physiological functions. They are widely used for modeling and personalized drug screening physiological functions.2025-02-06
