H-Gly-Pro-Hyp-OH  (Synonyms: Tripeptide 29)

H-Gly-Pro-Hyp-OH is a collagen-derived peptide and also a dipeptidyl peptidase-4 inhibitor.\nH-Gly-Pro-Hyp-OH inhibits the activity of dipeptidyl peptidase-4 in vitro. H-Gly-Pro-Hyp-OH binds to the DNA-binding site of the JUN/FOS complex, blocks the formation of the DNA-JUN/FOS complex, and inhibits transcriptional activity. H-Gly-Pro-Hyp-OH is applicable to research related to skin photoaging, UVB-induced skin aging/photoaging, and thrombosis^[1][2][3][4].

H-Gly-Pro-Hyp-OH (50-100 μg/mL; 24 h) reduces UVB-induced MMP-1 production and increases type 1 procollagen synthesis in human dermal fibroblast (HDF) cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

H-Gly-Pro-Hyp-OH (0.2 mmol/kg; p.o.; single dose) administered orally to male BALB/c mice results in transient accumulation of Gly-Pro-Hyp in plasma (C_max 14.78 nmol/mL) and skin (C_max 8.62 nmol/g), alongside hydrolysis to Pro-Hyp which reaches higher AUC_0-t values in both tissues^[1].
H-Gly-Pro-Hyp-OH (31.36 mg/kg per day; p.o.) exerts potent anti-photoaging activity in UV-irradiated Kunming mice, restoring skin elasticity, increasing hyaluronic acid and collagen content, reducing epidermal thickening, alleviating inflammation, and decreasing matrix metalloproteinase levels to a degree comparable to the high-Gly-Pro-Xaa collagen hydrolysate (HCH)^[2].
H-Gly-Pro-Hyp-OH (Metabolite after 1000 mg/kg CPNS; p.o.; single dose) shows only minimal, non-significant increases in plasma concentrations in Sprague-Dawley rats, with an AUC_0-t of 98.41 min∙nmol/mL over 180 minutes^[3].
H-Gly-Pro-Hyp-OH (Metabolite after 300-500 mg/kg/day CPNS; p.o.; daily; 12 weeks) has no direct pharmacodynamic outcomes specific to it reported for this UVB-induced photoaging mouse model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

285.30

C₁₂H₁₉N₃O₅

2239-67-0

Solid

White to off-white

Gly-Pro-{Hyp}

GP-{Hyp}

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Yazaki M, et al. Oral Ingestion of Collagen Hydrolysate Leads to the Transportation of Highly Concentrated Gly-Pro-Hyp and Its Hydrolyzed Form of Pro-Hyp into the Bloodstream and Skin. J Agric Food Chem. 2017;65(11):2315-2322.

  [2]. Yang D, et al. Effects of collagen hydrolysates on UV-induced photoaging mice: Gly-Pro-Hyp as a potent anti-photoaging peptide. Food Funct. 2024;15(6):3008-3022. Published 2024 Mar 18.

  [3]. Lee HJ, et al. Orally administered collagen peptide protects against UVB-induced skin aging through the absorption of dipeptide forms, Gly-Pro and Pro-Hyp. Biosci Biotechnol Biochem. 2019;83(6):1146-1156.  [Content Brief]

  [4]. Knight CG, et al. Collagen-platelet interaction: Gly-Pro-Hyp is uniquely specific for platelet Gp VI and mediates platelet activation by collagen. Cardiovasc Res. 1999;41(2):450-457.