xStAx-VHLL
Based on 3 publication(s) in Google Scholar
xStAx-VHLL is a β-catenin PROTAC degrader that promotes ubiquitination and proteasome degradation of β-catenin. xStAx-VHLL inhibits the Wnt/β-catenin signaling pathway. xStAx-VHLL can inhibit the proliferation of colon cancer cells and has anti-tumor activity.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.95%
- Formel: C154H244N48O29
- Molecular Weight:3231.89
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Speicherung:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) xStAx-VHLL
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Biologische Aktivität
Beschreibung
In Vitro
xStAx-VHLL (10-60 μM; 1-6 days) induces β-catenin degradation in a dose-dependent manner in colorectal cancer cells and inhibits cell proliferation[1].
xStAx-VHLL (50-90 μM; 24 h) inhibits Wnt/β-catenin signaling in HEK293T cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HCT116 cells
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Concentration:10, 20, 30, 40, 50 and 60 μM
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Incubation Time:24 h
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Result:Reduced the level of β-catenin in a dose-dependent manner.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:APC min/+ mice that harbored a point mutation in the APC gene, resulting in APC deletion and constitutive activation of Wnt signaling (APC min/+ mice develops intestinal tumors around 10 weeks-old) [1]
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Dosage:30 mg/kg
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Administration:Intraperitoneal injection (i.p.); 14 days
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Result:Dramatically reduced tumor number.
Reduced the active of β-catenin protein levels.
Chemical Information
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Appearance Solid
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Molecular Weight 3231.89
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Formel C154H244N48O29
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Color White to off-white
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Sequence
Ac-Arg-Arg-Trp-Pro-Arg-{S5}-Ile-Leu-Asp-{S5}-His-Val-Arg-Arg-Val-Trp-Arg-{Ahx}-Ala-Leu-Ala-Pro-Tyr-Ile-Pro-NH2
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Sequence Shortening
Ac-RRWPR{S5}ILD{S5}HVRRVWR-{Ahx}-ALAPYIP-NH2
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Publications (3)
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Journal Impact Factor
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Most Recent
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Platelets
Platelet-derived extracellular vesicles promote hair follicle growth through β-catenin signaling pathway. [Abstract]2025 Dec;36(1):2498353. PMID: 40323125 -
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Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (30.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Reinheit & Dokumentation
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Data Sheet (282 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 0.3094 mL | 1.5471 mL | 3.0942 mL | 7.7354 mL |
| 5 mM | 0.0619 mL | 0.3094 mL | 0.6188 mL | 1.5471 mL | |
| 10 mM | 0.0309 mL | 0.1547 mL | 0.3094 mL | 0.7735 mL | |
| 15 mM | 0.0206 mL | 0.1031 mL | 0.2063 mL | 0.5157 mL | |
| 20 mM | 0.0155 mL | 0.0774 mL | 0.1547 mL | 0.3868 mL | |
| 25 mM | 0.0124 mL | 0.0619 mL | 0.1238 mL | 0.3094 mL | |
| 30 mM | 0.0103 mL | 0.0516 mL | 0.1031 mL | 0.2578 mL |
xStAx-VHLL Related Classifications
HY-P5819 Related Classifications
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