Z-Leu-Arg-AMC
Based on 1 Customer Validation
Z-Leu-Arg-AMC is a fluorogenic peptide substrate for cysteine proteases (e.g., Cathepsin) (Ex=350 nm，Em=460 nm). Z-Leu-Arg-AMC is preferentially cleaved by Cathepsin K and S under weakly acidic conditions, while its hydrolysis relies on residual Cathepsin S activity at neutral pH. Z-Leu-Arg-AMC serves as a substrate for recombinant Sphenophorus levis Cathepsin L, falcipain-2, falcipain-3, berghepain-2, knowlepain-2, vivapain-2, as well as falcipain-2 chimeras and constructs. It enables quantitative detection of cysteine protease activity in human inflammatory bronchoalveolar lavage fluid via fluorescence generation. Z-Leu-Arg-AMC can be used in research related to pulmonary inflammatory diseases and malaria.
For research use only. We do not sell to patients.
- Purity: 99.24%
- CAS No.: 156192-32-4
- Formula: C30H38N6O6
- Molecular Weight:578.66
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Trypanosoma |
cathepsin K |
cathepsin S |
cathepsin L |
Z-Leu-Arg-AMC (25 μM; continuous/1 h) is hydrolysed by active cysteine proteases in cell-free supernatants from human inflammatory BALFs, with residual activity at neutral pH specific to cathepsin S[1].
Z-Leu-Arg-AMC is a substrate for refolded active falcipain-2 constructs (4TyrFP2, 19AspFP2Active) that show activity against it in solution and exhibit gelatinolytic activity in native but not denaturing gel systems[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 156192-32-4
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Appearance Solid
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Molecular Weight 578.66
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Formula C30H38N6O6
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Color White to off-white
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SMILES
CC(C1=CC=C(NC([C@H](CCCNC(N)=N)NC([C@H](CC(C)C)NC(OCC2=CC=CC=C2)=O)=O)=O)C=C1O3)=CC3=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL (172.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[2]. Fonseca FP, et al. Recombinant expression, localization and in vitro inhibition of midgut cysteine peptidase (Sl-CathL) from sugarcane weevil, Sphenophorus levis. Insect Biochem Mol Biol. 2012;42(1):58-69. [Content Brief]
[3]. Galibert M, et al. Substrate-derived triazolo- and azapeptides as inhibitors of cathepsins K and S. Eur J Med Chem. 2018;144:201-210. [Content Brief]
[4]. Pandey KC, et al. Independent intramolecular mediators of folding, activity, and inhibition for the Plasmodium falciparum cysteine protease falcipain-2. J Biol Chem. 2004;279(5):3484-3491. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7281 mL | 8.6407 mL | 17.2813 mL | 43.2033 mL |
| 5 mM | 0.3456 mL | 1.7281 mL | 3.4563 mL | 8.6407 mL | |
| 10 mM | 0.1728 mL | 0.8641 mL | 1.7281 mL | 4.3203 mL | |
| 15 mM | 0.1152 mL | 0.5760 mL | 1.1521 mL | 2.8802 mL | |
| 20 mM | 0.0864 mL | 0.4320 mL | 0.8641 mL | 2.1602 mL | |
| 25 mM | 0.0691 mL | 0.3456 mL | 0.6913 mL | 1.7281 mL | |
| 30 mM | 0.0576 mL | 0.2880 mL | 0.5760 mL | 1.4401 mL | |
| 40 mM | 0.0432 mL | 0.2160 mL | 0.4320 mL | 1.0801 mL | |
| 50 mM | 0.0346 mL | 0.1728 mL | 0.3456 mL | 0.8641 mL | |
| 60 mM | 0.0288 mL | 0.1440 mL | 0.2880 mL | 0.7201 mL | |
| 80 mM | 0.0216 mL | 0.1080 mL | 0.2160 mL | 0.5400 mL | |
| 100 mM | 0.0173 mL | 0.0864 mL | 0.1728 mL | 0.4320 mL |
Z-Leu-Arg-AMC Related Classifications
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