1. Cell Cycle/DNA Damage
  2. Deubiquitinase
  3. C527

C527 

Cat. No.: HY-12988 Purity: 98.92%
Handling Instructions

C527 is a is a pan DUB enzyme inhibitor, with a high potency for the USP1/UAF1 complex (IC50=0.88 μM).

For research use only. We do not sell to patients.

C527 Chemical Structure

C527 Chemical Structure

CAS No. : 192718-06-2

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Estimated Time of Arrival: December 31
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Description

C527 is a is a pan DUB enzyme inhibitor, with a high potency for the USP1/UAF1 complex (IC50=0.88 μM).

IC50 & Target

0.88 μM (USP1)[1]

In Vitro

Pretreatment of USP1/UAF1 with C527 resulted in inhibition of its enzyme activity with an IC50 of 0.88±0.03 μM. C527 inhibits the DUB activity of the USP12/USP46 complex and other DUB enzymes in vitro. However, the IC50 of C527 for these DUB enzymes was higher in comparison with USP1/UAF1 complex. C527 has considerably less inhibitory effect on UCH-L1 and UCH-L3, a different subclass of DUB enzymes. C527 treatments causes an increase in the levels of Ub-FANCD2 and Ub-FANCI. Pretreatment of cells with the C527 causes an enhancement in the cytoxicity of mitomycin C and camptothecin. C527 treatments lead to an increase in ubiquitinated forms of FANCD2 and FANCI, cause a decrease in homologous recombination activity, and sensitize cells to DNA damaging agents[1].

Storage

4°C, protect from light

Solvent & Solubility
In Vitro: 

DMSO : < 1 mg/mL (insoluble or slightly soluble)

References
Cell Assay
[1]

HeLa cells are treated with DMSO or C527 (0.5, 1, 5 μM) in appropriate medium for 24 to 72 hours. The viable cell counts are determined using Trypan Blue staining, CellTiter-Glo reagent or MTT assay[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

293.25

Formula

C₁₇H₈FNO₃

CAS No.

192718-06-2

SMILES

O=C1C2=C(N=C(C3=CC=C(F)C=C3)O2)C(C4=CC=CC=C41)=O

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
C527
Cat. No.:
HY-12988
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C527

Cat. No.: HY-12988