GRGDSPK
Based on 1 Customer Validation
GRGDSPK (EMD 56574) is a peptide incluing Arg-Gly-Asp (RGD). GRGDSPK (EMD 56574) is an competitive and reversible inhibitory peptide for inhibiting integrin-fibronectin binding. GRGDSPK is used to study the role of integrins in bone formation and resorption.
For research use only. We do not sell to patients.
- Purity: 98.55%
- CAS No.: 111119-28-9
- Formula: C28H49N11O11
- Molecular Weight:715.76
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
GRGDSPK (EMD 56574; RGD; 0.1-50 μM; for 4 days) inhibits mineralization in a dose-dependent manner as determined by measuring calcium content and 70 bonehnit area of tissue in parietal bones 18 days old isolated from pregnant Sprague-Dawley rats[1].
GRGDSPK (10, 50 μM; for 4 days) dramatically alteres bone morphology, with a disruption of osteoblast and mineralized matrix organization[1].
GRGDSPK (RGD; 250 μM), added to the medium, effectively blocks integrin-fibronectin binding and significantly increases the average size of wild-type cell aggregates[2].
When GRGDSPK (250 μM) is added, wild-type mesendodermal progenitors exhibits strongly reduced adhesion forces and work, indicating that the detachment parameters recorded are specific for fibronectin and that integrins expressed in mesendodermal progenitors are involved[2].
GRGDSPK (RGD-containing, 1.5 mM, 1.0 mM, and 0.5 mM) and RGD-mod-ified peptides impair the ability of sperm to fertilizebovine oocytes, illustrated by a reduction in cleavage in a dose-dependent manner[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 111119-28-9
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Appearance Solid
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Molecular Weight 715.76
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Formula C28H49N11O11
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Color White to off-white
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Synonyms
EMD 56574
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Sequence
Gly-Arg-Gly-Asp-Ser-Pro-Lys
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Sequence Shortening
GRGDSPK
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : ≥ 100 mg/mL (139.71 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
Proliferation assays are carried out in 48-well cell culture dishes coated with OCP or HL. In proliferation assays, 7.25×103 cells in 0.5 mL of culture medium are seeded in a 48-well dish. Culture medium is changed every 3 days. After 1, 6, 12, and 18 days of culture, plates are washed thrice with PBS to remove unattached cells, and attached cells are collected by trypsinization. The number of cells is counted using a hemocytometer.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. G A Gronowicz, et al. Synthetic Peptide Containing Arg-Gly-Asp Inhibits Bone Formation and Resorption in a Mineralizing Organ Culture System of Fetal Rat Parietal Bones. J Bone Miner Res. 1994 Feb;9(2):193-201. [Content Brief]
[2]. Pierre-Henri Puech, et al. Measuring Cell Adhesion Forces of Primary Gastrulating Cells From Zebrafish Using Atomic Force Microscopy. J Cell Sci. 2005 Sep 15;118(Pt 18):4199-206. [Content Brief]
[3]. Sessions BR, et al. Effects of amino acid substitutions in and around the arginine-glycine-aspartic acid (RGD) sequence on fertilization and parthenogenetic development in mature bovine oocytes. Mol Reprod Dev. 2006 May;73(5):651-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.3971 mL | 6.9856 mL | 13.9712 mL | 34.9279 mL |
| 5 mM | 0.2794 mL | 1.3971 mL | 2.7942 mL | 6.9856 mL | |
| 10 mM | 0.1397 mL | 0.6986 mL | 1.3971 mL | 3.4928 mL | |
| 15 mM | 0.0931 mL | 0.4657 mL | 0.9314 mL | 2.3285 mL | |
| 20 mM | 0.0699 mL | 0.3493 mL | 0.6986 mL | 1.7464 mL | |
| 25 mM | 0.0559 mL | 0.2794 mL | 0.5588 mL | 1.3971 mL | |
| 30 mM | 0.0466 mL | 0.2329 mL | 0.4657 mL | 1.1643 mL | |
| 40 mM | 0.0349 mL | 0.1746 mL | 0.3493 mL | 0.8732 mL | |
| 50 mM | 0.0279 mL | 0.1397 mL | 0.2794 mL | 0.6986 mL | |
| 60 mM | 0.0233 mL | 0.1164 mL | 0.2329 mL | 0.5821 mL | |
| 80 mM | 0.0175 mL | 0.0873 mL | 0.1746 mL | 0.4366 mL | |
| 100 mM | 0.0140 mL | 0.0699 mL | 0.1397 mL | 0.3493 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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