GRGDSPK (Synonyms: EMD 56574)

Name: GRGDSPK
Brand: MedChemExpress
SKU: HY-P0322
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Art. -Nr.: HY-P0322 Reinheit: 98.55%: Data Sheet
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Handling Instructions
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GRGDSPK (EMD 56574) is a peptide incluing Arg-Gly-Asp (RGD). GRGDSPK (EMD 56574) is an competitive and reversible inhibitory peptide for inhibiting integrin-fibronectin binding. GRGDSPK is used to study the role of integrins in bone formation and resorption.

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Kundenspezifische Peptidsynthese

GRGDSPK Chemische Struktur

CAS. Nr. : 111119-28-9

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1 mg	Auf Lager	Estimated Time of Arrival: December 31
5 mg	Auf Lager	Estimated Time of Arrival: December 31
10 mg	Auf Lager	Estimated Time of Arrival: December 31
25 mg	Auf Lager	Estimated Time of Arrival: December 31
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Other Forms of GRGDSPK:

GRGDSPK TFA Angebot einholen

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Alle Integrin Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen

αvβ1 αvβ3 αvβ5 αvβ6 αvβ8 α5β1 α1β1 α2β1 α4β1 α9β1 αIIbβ3 α4β7 αLβ2

Biologische Aktivität
Protokoll
Reinheit & Dokumentation
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Beschreibung

In Vitro

GRGDSPK (EMD 56574; RGD; 0.1-50 μM; for 4 days) inhibits mineralization in a dose-dependent manner as determined by measuring calcium content and 70 bonehnit area of tissue in parietal bones 18 days old isolated from pregnant Sprague-Dawley rats^[1].
GRGDSPK (10, 50 μM; for 4 days) dramatically alteres bone morphology, with a disruption of osteoblast and mineralized matrix organization^[1].
GRGDSPK (RGD; 250 μM), added to the medium, effectively blocks integrin-fibronectin binding and significantly increases the average size of wild-type cell aggregates^[2].
When GRGDSPK (250 μM) is added, wild-type mesendodermal progenitors exhibits strongly reduced adhesion forces and work, indicating that the detachment parameters recorded are specific for fibronectin and that integrins expressed in mesendodermal progenitors are involved^[2].
GRGDSPK (RGD-containing, 1.5 mM, 1.0 mM, and 0.5 mM) and RGD-mod-ified peptides impair the ability of sperm to fertilizebovine oocytes, illustrated by a reduction in cleavage in a dose-dependent manner^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molekulargewicht

715.76

Formel

C₂₈H₄₉N₁₁O₁₁

CAS. Nr.

111119-28-9

Appearance

Solid

Color

White to off-white

Sequence

Gly-Arg-Gly-Asp-Ser-Pro-Lys

Sequence Shortening

GRGDSPK

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : ≥ 100 mg/mL (139.71 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3971 mL	6.9856 mL	13.9712 mL
5 mM	0.2794 mL	1.3971 mL	2.7942 mL
10 mM	0.1397 mL	0.6986 mL	1.3971 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner
Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Reinheit & Dokumentation

Purity: 98.55%

Select Batch:

Data Sheet (277 KB) SDS (254 KB)

COA (252 KB) RP-HPLC (111 KB) MS (215 KB) Elemental Analysis Report (111 KB)

Handling Instructions (2659 KB)

Verweise

[1]. G A Gronowicz, et al. Synthetic Peptide Containing Arg-Gly-Asp Inhibits Bone Formation and Resorption in a Mineralizing Organ Culture System of Fetal Rat Parietal Bones. J Bone Miner Res. 1994 Feb;9(2):193-201. [Content Brief]

[2]. Pierre-Henri Puech, et al. Measuring Cell Adhesion Forces of Primary Gastrulating Cells From Zebrafish Using Atomic Force Microscopy. J Cell Sci. 2005 Sep 15;118(Pt 18):4199-206. [Content Brief]

[3]. Sessions BR, et al. Effects of amino acid substitutions in and around the arginine-glycine-aspartic acid (RGD) sequence on fertilization and parthenogenetic development in mature bovine oocytes. Mol Reprod Dev. 2006 May;73(5):651-7. [Content Brief]

Zellassay
^[1]

Proliferation assays are carried out in 48-well cell culture dishes coated with OCP or HL. In proliferation assays, 7.25×10³ cells in 0.5 mL of culture medium are seeded in a 48-well dish. Culture medium is changed every 3 days. After 1, 6, 12, and 18 days of culture, plates are washed thrice with PBS to remove unattached cells, and attached cells are collected by trypsinization. The number of cells is counted using a hemocytometer.

MCE hat die Genauigkeit dieser Methoden nicht unabhängig bestätigt. Sie dienen nur als Referenz.

Verweise

[1]. G A Gronowicz, et al. Synthetic Peptide Containing Arg-Gly-Asp Inhibits Bone Formation and Resorption in a Mineralizing Organ Culture System of Fetal Rat Parietal Bones. J Bone Miner Res. 1994 Feb;9(2):193-201. [Content Brief]

[2]. Pierre-Henri Puech, et al. Measuring Cell Adhesion Forces of Primary Gastrulating Cells From Zebrafish Using Atomic Force Microscopy. J Cell Sci. 2005 Sep 15;118(Pt 18):4199-206. [Content Brief]

[3]. Sessions BR, et al. Effects of amino acid substitutions in and around the arginine-glycine-aspartic acid (RGD) sequence on fertilization and parthenogenetic development in mature bovine oocytes. Mol Reprod Dev. 2006 May;73(5):651-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.3971 mL	6.9856 mL	13.9712 mL	34.9279 mL
	5 mM	0.2794 mL	1.3971 mL	2.7942 mL	6.9856 mL
	10 mM	0.1397 mL	0.6986 mL	1.3971 mL	3.4928 mL
	15 mM	0.0931 mL	0.4657 mL	0.9314 mL	2.3285 mL
	20 mM	0.0699 mL	0.3493 mL	0.6986 mL	1.7464 mL
	25 mM	0.0559 mL	0.2794 mL	0.5588 mL	1.3971 mL
	30 mM	0.0466 mL	0.2329 mL	0.4657 mL	1.1643 mL
	40 mM	0.0349 mL	0.1746 mL	0.3493 mL	0.8732 mL
	50 mM	0.0279 mL	0.1397 mL	0.2794 mL	0.6986 mL
	60 mM	0.0233 mL	0.1164 mL	0.2329 mL	0.5821 mL
	80 mM	0.0175 mL	0.0873 mL	0.1746 mL	0.4366 mL
	100 mM	0.0140 mL	0.0699 mL	0.1397 mL	0.3493 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

GRGDSPK Related Classifications

Metabolic Disease Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GRGDSPK111119-28-9EMD 56574EMD56574EMD-56574IntegrinArg-Gly-AspRGDintegrinfibronectinbindingboneformationresorptionInhibitorinhibitorinhibit

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GRGDSPK (Synonyms: EMD 56574)

Kundenbewertung

Other Forms of GRGDSPK:

GRGDSPK Related Antibodies

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Alle Integrin Isoform-spezifische Produkte anzeigen:

Biologische Aktivität

Protokoll

Reinheit & Dokumentation

Verweise

Kundenbewertung

GRGDSPK Related Antibodies

Molaritätsrechner

Verdünnungsrechner

Complete Stock Solution Preparation Table

GRGDSPK Related Classifications

Keywords:

Ihre kürzlich angesehenen Produkte:

Kundenbewertung

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SAFETY DATA SHEET (SDS)

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