Schisandrol B
Based on 2 publication(s) in Google Scholar
Schisandrol B (Gomisin-A) is a major active constituent of Schisandra chinensis with hepato-protective effects. Schisandrol B inhibits reactive oxygen species (ROS) production. Schisandrol B inhibits the activity of P-glycoprotein and CYP3A and also has anti-inflammatory, anti-diabetic and antioxidant activities.
For research use only. We do not sell to patients.
- Purity : 99.92%
- CAS No.: 58546-54-6
- Formula: C23H28O7
- Molecular Weight:416.46
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Schisandrol B
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Customer Validation & Images
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WB
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RT-PCR
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IF
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Histological Imaging/Staining
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IHC
Biological Activity
Description
IC50 & Target
[3]|
CYP2 |
CYP3A |
In Vitro
Schisandrol B (Gomisin-A; 1-10 μM; 2 days) treatment decreases the aging related inflammatory molecules, such as, COX-2, IL1β, and TNF-α. Schisandrol B attenuates the activity of senescence-associated β-galactosidase[2].
Schisandrol B (Gomisin-A; 1-10 μM; 2 days) inhibits reactive oxygen species production even in the stress-induced premature senescence (SIPS)-human diploid fibroblast (HDF) cells[2].
Schisandrol B (Gomisin-A; 1-10 μM) inhibits the MAPK pathway and the translocation of NF-κB to the nucleus[2].
Schisandrol B (Gomisin-A; 1-10 μM) promotes the autophagy and mitochondrial biogenesis factors through the translocation of Nrf-2, and inhibits aging progression in the SIPS-HDF cells[2].
Schisandrol B (0-80?μM) dramatically alters APAP metabolic activation by inhibiting the activities of CYP2E1 and CYP3A11[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Human diploid fibroblast (HDF) cells
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Concentration:1 μM, 10 μM
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Incubation Time:3 days
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Result:Decreased the aging related inflammatory molecules, such as, COX-2, IL1β, and TNF-α.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male C57BL/6 mice (6-8 weeks old, 20-22 g) injected with Acetaminophen (APAP)[1]
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Dosage:12.5 mg/kg, 12.5 mg/kg and 200 mg/kg
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Administration:Oral administration; seven times with an interval of 12 hours
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Result:Showed a protective effect against APAP-induced liver injury in mice.
Chemical Information
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CAS No. 58546-54-6
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Appearance Solid
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Molecular Weight 416.46
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Formula C23H28O7
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Color White to off-white
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SMILES
O[C@@]([C@@H](C)C1)(C)CC2=CC(OC)=C(OC)C(OC)=C2C3=C1C=C4OCOC4=C3OC
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Synonyms
Gomisin-A; TJN-101; Wuweizi alcohol-B
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (2)
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Journal Impact Factor
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Most Recent
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Front Cell Dev Biol
Curdione and Schisandrin C Synergistically Reverse Hepatic Fibrosis via Modulating the TGF-β Pathway and Inhibiting Oxidative Stress. [Abstract]2021 Nov 10;9:763864. PMID: 34858986 -
Biomed Pharmacother
Schisandrol B inhibits calcification of aortic valve by targeting p53 related inflammatory and senescence. [Abstract]2024 Sep:178:117241. PMID: 39111082
Schisandrol B purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Sep:178:117241. [Abstract]
WB analysis of ALP and Runx2 after different concentrations of Schisandrol B (SolB) (10 μM) treatment in OM induced osteogenic differentiation of hVICs.
Schisandrol B purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Sep:178:117241. [Abstract]
Gene expression of ALP, RUNX2, OPN was detected by qRT-PCR treated with Schisandrol B (SolB) (10 μM).
Schisandrol B purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Sep:178:117241. [Abstract]
Immunofluorescent staining of Runx2 and Dapi, semiquantitative analysis of Runx2 fluorescence intensity under OM inducement with or without Schisandrol B (SolB) (10 μM) for 48 hours.
Schisandrol B purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Sep:178:117241. [Abstract]
Images of HE staining, Masson staining and Von Kossa staining Schisandrol B (SolB) (100 mg/kg/day, i.g.).
Schisandrol B purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Sep:178:117241. [Abstract]
Representative images of immunohistochemical staining for IL-6, IL-17, MCP1 and TNF-α in valve tissue of wire-injured murine model treated with Schisandrol B (SolB) (100 mg/kg/day, i.g.).
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (120.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.00 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (6.00 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
Purity & Documentation
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Data Sheet (286 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Jiang Y, et al. Schisandrol B protects against acetaminophen-induced hepatotoxicity by inhibition of CYP-mediated bioactivation and regulation of liver regeneration. Toxicol Sci. 2015 Jan;143(1):107-15. [Content Brief]
[2]. Jeong-Seok Kim, et al. Gomisin A modulates aging progress via mitochondrial biogenesis in human diploid fibroblast cells. Clin Exp Pharmacol Physiol. 2018 Jun;45(6):547-555. [Content Brief]
[3]. Jin J, et al. Enhancement of oral bioavailability of paclitaxel after oral administration of Schisandrol B in rats. Biopharm Drug Dispos. 2010 May;31(4):264-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.4012 mL | 12.0060 mL | 24.0119 mL | 60.0298 mL |
| 5 mM | 0.4802 mL | 2.4012 mL | 4.8024 mL | 12.0060 mL | |
| 10 mM | 0.2401 mL | 1.2006 mL | 2.4012 mL | 6.0030 mL | |
| 15 mM | 0.1601 mL | 0.8004 mL | 1.6008 mL | 4.0020 mL | |
| 20 mM | 0.1201 mL | 0.6003 mL | 1.2006 mL | 3.0015 mL | |
| 25 mM | 0.0960 mL | 0.4802 mL | 0.9605 mL | 2.4012 mL | |
| 30 mM | 0.0800 mL | 0.4002 mL | 0.8004 mL | 2.0010 mL | |
| 40 mM | 0.0600 mL | 0.3001 mL | 0.6003 mL | 1.5007 mL | |
| 50 mM | 0.0480 mL | 0.2401 mL | 0.4802 mL | 1.2006 mL | |
| 60 mM | 0.0400 mL | 0.2001 mL | 0.4002 mL | 1.0005 mL | |
| 80 mM | 0.0300 mL | 0.1501 mL | 0.3001 mL | 0.7504 mL | |
| 100 mM | 0.0240 mL | 0.1201 mL | 0.2401 mL | 0.6003 mL |
Schisandrol B Related Classifications
HY-N0692 Related Classifications
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