AG-12286
AG-12286 is a pan-CDK inhibitor with an IC50 value of 2 nM against cdk1/cyclin B, 6 nM against cdk2/cyclin E, and 12 nM against cdk4/cyclin D. AG-12286 is 1000-fold more selective for the CDK family than for PKC. AG-12286 can be used in cancer research.
For research use only. We do not sell to patients.
- CAS No.: 223784-75-6
- Formula: C16H12F2N4O3S2
- Molecular Weight:410.42
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
IC50 & Target
[1]|
Cdk1/cyclin B 2 nM (IC50) |
CDK2/cyclinE 6 nM (IC50) |
CDK4/cyclin D 12 nM (IC50) |
In Vitro
AG-12286 acts as a pan-cyclin-dependent kinase inhibitor, potently inhibiting cdk1/cycB (IC50 = 2 nM), cdk2/cycE (IC50 = 6 nM), and cdk4/cycD (IC50 = 12 nM) while showing >1000X selectivity over PKC[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 223784-75-6
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Molecular Weight 410.42
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Formula C16H12F2N4O3S2
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SMILES
O=C(C1=C(F)C=CC=C1F)C2=C(N)N=C(NC3=CC=C(S(=O)(N)=O)C=C3)S2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)