Cat. No.: HY-175646

AGPAT4-IN-1

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Based on 1 Customer Validation

AGPAT4-IN-1 (Compound CL26) is a covalent AGPAT4 inhibitor with an IC₅₀ of 795 nM. AGPAT4-IN-1 covalently binds to AGPAT4 at Cys²²⁸ and significantly inhibits acyltransferase activity, LPA-to-PA conversion and downstream mTOR/S6K pathways. AGPAT4-IN-1 sensitizes hepatocellular carcinoma (HCC) tumors to Sorafenib (HY-10201) and significantly induces apoptosis with a synergistic response. AGPAT4-IN-1 has antitumor activity and reduces tumorigenicity and stemness in HCC xenograft mouse models.

For research use only. We do not sell to patients.

Purity: 99.04%
CAS No.: 2411250-59-2
Formula: C₁₄H₁₁ClN₂O₂S
Molecular Weight:306.77

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

AGPAT4-IN-1 Chemical Structure

CAS No.: 2411250-59-2

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Use of MCE Products

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Acyltransferase Isoforms :

All mTOR Isoforms :

All Ribosomal S6 Kinase (RSK) Isoforms :

Chemical Information

CAS No. 2411250-59-2
Appearance Solid
Molecular Weight 306.77
Formula C₁₄H₁₁ClN₂O₂S
Color Light yellow to yellow
SMILES

O=C(CCl)NCC(O1)=CC=C1C(S2)=NC3=C2C=CC=C3
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (325.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.2598 mL

16.2989 mL

32.5977 mL

5 mM

0.6520 mL

3.2598 mL

6.5195 mL

10 mM

0.3260 mL

1.6299 mL

3.2598 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (16.30 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ng KY, et al. AGPAT4 targeted covalent inhibitor potentiates targeted therapy to overcome cancer cell plasticity in hepatocellular carcinoma mouse models. Sci Transl Med. 2025 Jul 30;17(809):eadn9472. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.2598 mL	16.2989 mL	32.5977 mL	81.4943 mL
	5 mM	0.6520 mL	3.2598 mL	6.5195 mL	16.2989 mL
	10 mM	0.3260 mL	1.6299 mL	3.2598 mL	8.1494 mL
	15 mM	0.2173 mL	1.0866 mL	2.1732 mL	5.4330 mL
	20 mM	0.1630 mL	0.8149 mL	1.6299 mL	4.0747 mL
	25 mM	0.1304 mL	0.6520 mL	1.3039 mL	3.2598 mL
	30 mM	0.1087 mL	0.5433 mL	1.0866 mL	2.7165 mL
	40 mM	0.0815 mL	0.4075 mL	0.8149 mL	2.0374 mL
	50 mM	0.0652 mL	0.3260 mL	0.6520 mL	1.6299 mL
	60 mM	0.0543 mL	0.2716 mL	0.5433 mL	1.3582 mL
	80 mM	0.0407 mL	0.2037 mL	0.4075 mL	1.0187 mL
	100 mM	0.0326 mL	0.1630 mL	0.3260 mL	0.8149 mL

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AGPAT4-IN-1

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High-Throughput Bioactive Compound Library

Lactylation Compound Library

All Acyltransferase Isoforms

All mTOR Isoforms

All Ribosomal S6 Kinase (RSK) Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

AGPAT4-IN-1 Related Classifications

HY-175646 Related Classifications