Anti-Human/Mouse denatured collagen type-I Antibody (XL313)
Based on 1 Customer Validation
Anti-Human/Mouse denatured collagen type-I Antibody (XL313) is a mouse-derived IgG1 κ type antibody inhibitor, targeting to human/mouse denatured collagen type-I. Anti-Human/Mouse denatured collagen type-I Antibody (XL313) selectively binds to proteolyzed collagen type I. Anti-Human/Mouse denatured collagen type-I Antibody (XL313) reduces PD L1 levels in tumor cells. Anti-Human/Mouse denatured collagen type-I Antibody (XL313) can be used for the researches of cancer and inflammation, such as such as ovarian tumor.
For research use only. We do not sell to patients.
- Purity : ≥95%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Mouse IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Human/Mouse
In Vitro
Anti-Human/Mouse denatured collagen type-I Antibody (XL313) (16 h) exhibits selective binding for proteolyzed collagen type I[1].
Anti-Human/Mouse denatured collagen type-I Antibody (XL313) (10 μg, daily for 3 times) significantly inhibits the number of FGF-2-induced chick chorioallantoic membranes exhibiting inflammation and reduces the levels of inflammatory infiltrates[1].
Anti-Human/Mouse denatured collagen type-I Antibody (XL313) (10 μg/mL, 24 h) shows no cytotoxicity in Β16F10 melanoma cells[2].
Anti-Human/Mouse denatured collagen type-I Antibody (XL313) (100 μg/mL, 15 mins or 1 h) reduces PD L1 and nuclear YAP levels by a proteasome dependent mechanism in Β16F10, YUMM1.7, C32 and A375 tumor cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Β16F10, YUMM1.7, C32 and A375 tumor cells
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Concentration:100 μg/mL
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Incubation Time:15 min or 1 h
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Result:Reduced PD L1 and nuclear YAP levels.
In Vivo
Anti-Human/Mouse denatured collagen type-I Antibody (XL313) (100 μg, i.p., 1-2 times a week) shows anti-tumor effect in intraperitoneal ID8 tumor and ID8-VEGF ovarian tumor mice models[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Β16F10/4T1 tumor mice models[2]
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Dosage:250 μg
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Administration:Intraperitoneally injection, 3 times a week for 14 or 21 days
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Result:Inhibited tumor growth.
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Animal Model:Intraperitoneal ID8 tumor mice models[3]
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Dosage:100 μg
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Administration:Intraperitoneally injection, once per week for 7 weeks
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Result:Showed a significant inhibition of approximately 80% in the tumor burden.
Reduced CD4+ T cells, F4/80 expressing macrophages and PDGFRa expressing cells in spleens.
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Animal Model:ID8-VEGF ovarian tumor mice models[3]
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Dosage:100 μg
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Administration:Intraperitoneally injection, twice per week
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Result:Inhibited the growth of ID8-VEGF ovarian tumors.
Significantly inhibited the binding of the RGDKGE containing collagen fragment to ID8-VEGF cells.
Reduced abdominal distension and ascites volume.
Reduced levels of LAG-3 expressing cells.
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
Western blot; Immunofluorescence; in vivo administration
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Human/Mouse denatured collagen type-I Antibody (XL313)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
Purity & Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Ames JJ, et al. Identification of an Endogenously Generated Cryptic Collagen Epitope (XL313) That May Selectively Regulate Angiogenesis by an Integrin Yes-associated Protein (YAP) Mechano-transduction Pathway. J Biol Chem. 2016 Feb 5;291(6):2731-50. [Content Brief]
[2]. Caron JM, et al. Targeting the secreted RGDKGE collagen fragment reduces PD‑L1 by a proteasome‑dependent mechanism and inhibits tumor growth. Oncol Rep. 2023 Feb;49(2):44. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Human/Mouse denatured collagen type-I Antibody (XL313) Related Classifications
HY-P990251 Related Classifications
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