Bactenecin
Bactenecin (Bactenecin, bovine) is a potent 12-aa looped antimicrobial peptide isolated from bovine neutrophils. Bactenecin inhibits the growth of bacteria and yeast, and kills the fungus Trichophyton rubrum. Bactenecin increass membrane permeability, inhibits the growth and biofilm formation of B. pseudomallei.
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- CAS. Nr.: 116229-36-8
- Formel: C63H118N24O13S2
- Molecular Weight:1483.89
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Chemical Information
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- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
Bacteria; Yeast; Fungus[1]
Bactenecin is synthesized by solid-phase peptide synthesis and renatured to a fully disulfide bonded form. Bactenecin inhibits the growth of Escherichia coli and Staphylococcus aureus at the same concentration reported for the peptide purified from bovine neutrophils. Bactenecin inhibits the growth of other medically important bacteria and yeast, and it kills the fungus Trichophyton rubrum[1].
Bactenecin, a dodecapeptide, is strongly cytotoxic to rat embryonic neurons, fetal rat astrocytes and human glioblastoma cells[2].
Bactenecin exhibits stronger binding to LPS and induces perturbation of the inner membrane of live cells. Interaction of Bactenecin with model membranes results in changes in membrane fluidity and permeability, leading to leakage of dye across the membrane[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 116229-36-8
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Molecular Weight 1483.89
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Formel C63H118N24O13S2
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Synonyms
Bactenecin, bovine
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Sequence
Arg-Leu-Cys-Arg-Ile-Val-Val-Ile-Arg-Val-Cys-Arg (Disulfide bridge: Cys3-Cys11)
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Sequence Shortening
RLCRIVVIRVCR (Disulfide bridge: Cys3-Cys11)
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Structure Classification
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Initial Source
bovine neutrophils
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
Rat neuronal cells (5000 cells/well) are incubated for 24 hr with bactenecin (0.3, 0.6, 1.25, 2.5, 5, 10, 20, 40 μg/mL) or the control peptide. Cytotoxicity is assayed in 96-well tissue culture plates using a commercially available kit. The method is based on cellular reduction of tetrazolium salt into intensely coloured formazan derivates.[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Reinheit & Dokumentation
Verweise
[1]. Gallis B, et al. Antimicrobial activity of synthetic bactenecin. Biotechnol Ther. 1989-1990;1(4):335-46. [Content Brief]
[2]. Radermacher SW, et al. Bactenecin, a leukocytic antimicrobial peptide, is cytotoxic to neuronal and glial cells. J Neurosci Res. 1993 Dec 15;36(6):657-62. [Content Brief]
[3]. Kanjana Madhongsa, et al. Antimicrobial Action of the Cyclic Peptide Bactenecin on Burkholderia Pseudomallei Correlates With Efficient Membrane Permeabilization. PLoS Negl Trop Dis. 2013 Jun 13;7(6):e2267. [Content Brief]
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
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