Art. -Nr.: HY-B1102

Evans Blue

(Synonyms: Direct Blue 53; T-1824; C.I. 23860)

31 Cited Publications

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Based on 31 publication(s) in Google Scholar

Evans Blue (Direct Blue 53) is a potent inhibitor of L-glutamate uptake via the membrane bound excitatory amino acid transporter (EAAT). Evans Blue is a L-glutamate and kainate receptor-mediated currents inhibitor. Evans Blue has a strong affinity towards serum albumin, making it a high molecular weight protein tracer. Evans Blue is also used to study BBB (blood-brain barrier) permeability.

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Reinheit: 99.86%
CAS. Nr.: 314-13-6
Formel: C₃₄H₂₄N₆Na₄O₁₄S₄
Molecular Weight:960.81

Speicherung:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Evans Blue Chemical Structure

CAS. Nr.: 314-13-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (31)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle EAAT Isoform-spezifische Produkte anzeigen :

Alle iGluR Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
Sf9	IC₅₀	0.922 μM Compound: 20, NSC-8680	Inhibition of human recombinant C-terminal FLAG-tagged autotaxin expressed in baculovirus-infected Sf9 cells assessed as FS-3 hydrolysis Inhibition of human recombinant C-terminal FLAG-tagged autotaxin expressed in baculovirus-infected Sf9 cells assessed as FS-3 hydrolysis	[PMID: 20349977]

In Vitro

Evans blue, a bis-azo dye, is a non-permeating dye. In presence of plasma membrane damage, Evans blue enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The amount of Evans Blue (2% solution; 4 mL/kg) stain accumulated in the brains of mice after ICH produced by collagenase injection was independent of the stain administration route^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

CD-1 mice bearing intracerebral hemorrhage (ICH)^[2]
Dosage:

2% solution; 4 mL/kg
Administration:

Jugular vein or intraperitoneal injection; once
Result:

Showed the amount of stain accumulated in the brains.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 314-13-6
Appearance Solid
Molecular Weight 960.81
Formel C₃₄H₂₄N₆Na₄O₁₄S₄
Color Brown to black
SMILES

CC1=CC(C2=CC=C(/N=N/C3=C(O)C4=C(N)C(S(=O)(O[Na])=O)=CC(S(=O)(O[Na])=O)=C4C=C3)C(C)=C2)=CC=C1/N=N/C5=C(O)C6=C(N)C(S(=O)(O[Na])=O)=CC(S(=O)(O[Na])=O)=C6C=C5
Synonyms

Direct Blue 53; T-1824; C.I. 23860
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (31)

All In Vivo Efficacy StudyIF

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Journal Impact Factor

Cancer Cell

PDGFRα+ITGA11+ fibroblasts foster early-stage cancer lymphovascular invasion and lymphatic metastasis via ITGA11-SELE interplay. [Abstract]

2024 Apr 8;42(4):682-700.e12. PMID: 38428409
Nat Neurosci

Lipid-accumulated reactive astrocytes promote disease progression in epilepsy. [Abstract]

2023 Apr;26(4):542-554. PMID: 36941428
ACS Nano

Alleviating Pyroptosis of Intestinal Epithelial Cells to Restore Mucosal Integrity in Ulcerative Colitis by Targeting Delivery of 4-Octyl-Itaconate. [Abstract]

2024 Jul 2;18(26):16658-16673. PMID: 38907726
Nat Commun

Endothelial leakiness elicited by amyloid protein aggregation. [Abstract]

2024 Jan 19;15(1):613. PMID: 38242873
Adv Sci (Weinh)

The Thyroid Hormone Analog GC-1 Mitigates Acute Lung Injury by Inhibiting M1 Macrophage Polarization. [Abstract]

2024 Nov;11(44):e2401931. PMID: 39373388
Theranostics

Sufu limits sepsis-induced lung inflammation via regulating phase separation of TRAF6. [Abstract]

2023 Jun 26;13(11):3761-3780. PMID: 37441604

Evans Blue purchased from MedChemExpress. Usage Cited in: Theranostics. 2023 Jun 26;13(11):3761-3780. [Abstract]

Sufu-cKO and WT mice were intraperitoneally injected with PBS or LPS (5 mg/kg) for 23 h, then injected with Evans blue dye (30 mg/kg body weight; i.v.; single dose) for an additional 40 min before mice were killed and lung tissues harvested. Means ± SEM (n = 5).
Adv Healthc Mater

An Erythrocyte Membrane-Derived Nanosystem for Efficient Reversal of Endothelial Injury in Sepsis. [Abstract]

2024 Jan;13(3):e2302320. PMID: 37883686

Evans Blue purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2024 Jan;13(3):e2302320. [Abstract]

The effects of different groups (control, CLP, Ca-MOF, Zolif, CMZ, RCMZ, and γ3-RCMZ) on CLP-induced vascular permeability were examined by measuring Evans blue dye in peritoneal washings. Evans blue (10 mg/kg; i.v.; single dose) injection revealed that γ3-RCMZ effectively reduced endothelial leakage levels to one-sixth of those observed in the CLP group, indicating its predominant antiendothelial leakage effect in sepsis.
Proc Natl Acad Sci U S A

Binding adaptability of chemical ligands to polymorphic α-synuclein amyloid fibrils. [Abstract]

2024 Aug 27;121(35):e2321633121. PMID: 39172784
Int J Biol Macromol

Function of orthologous fibroblast growth factor 11 protein in angiogenesis and immunomodulatory after spinal cord injury. [Abstract]

2025 Oct 4;330(Pt 3):148106. PMID: 41052555

Evans Blue purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Oct 4;330(Pt 3):148106. [Abstract]

Representative images of injured spinal cord in the control, ZFgf11a, and MFGF11b groups after Evans Blue (EB, 2 mL/kg, i.v.; single dose) dye injection at 4 dpi.

Evans Blue purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Oct 4;330(Pt 3):148106. [Abstract]

Representative immunofluorescence images of Evans Blue (EB, 2 mL/kg, i.v.; single dose) extravasation at the lesion epicenter in the transverse sections of the spinal cord in the control, ZFgf11a, and MFGF11b groups at 4 dpi.
J Ethnopharmacol

2026 May 26:369:121916. PMID: 42202915
Cancer Immunol Immunother

TGF-β3 promotes vascular normalization of prostate cancer to potentiate immunotherapy and chemotherapy. [Abstract]

2025 Jul 12;74(8):268. PMID: 40650767

Evans Blue purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2025 Jul 12;74(8):268. [Abstract]

Representative images of Evans blue (4 ml/kg; i.v.; single dose) leakage and the difference between TGF-β3 and PBS groups (n = 5).
Respir Res

Human adenovirus type 7 (HAdV-7) infection induces pulmonary vascular endothelial injury through the activation of endothelial autophagy. [Abstract]

2024 Dec 4;25(1):425. PMID: 39633448
Int Immunopharmacol

Endothelium-targeted Ddx24 conditional knockout exacerbates ConA-induced hepatitis in mice due to vascular hyper-permeability. [Abstract]

2024 Mar 10:129:111618. PMID: 38354508
Mol Neurobiol

The Crosstalk Between Sepsis-Associated Encephalopathy and Alzheimer's Disease: Identifying Potential Biomarkers and Therapeutic Targets for Cognition. [Abstract]

2025 Jul 10. PMID: 40634788
J Inflamm Res

Lysophosphatidylcholine 14:0 Alleviates Lipopolysaccharide-Induced Acute Lung Injury via Protecting Alveolar Epithelial Barrier by Activation of Nrf2/HO-1 Pathway. [Abstract]

2024 Dec 6:17:10533-10546. PMID: 39659750
ACS Chem Neurosci

Developing Intravenous Delivery of Water-Soluble Prodrugs of Idebenone for the Treatment of Acute Ischemic Stroke. [Abstract]

2025 Sep 17;16(18):3541-3553. PMID: 40886294
Saudi Pharm J

Antifibrotic effect of the P2X7 receptor antagonist A740003 against acute myocardial infarction-induced fibrotic remodelling. [Abstract]

2024 Jul;32(7):102102. PMID: 39035363
J Intensive Med

Overexpression of Parkin promotes the protective effect of mitochondrial autophagy on the lung of rats with exertional heatstroke. [Abstract]

2024 Sep 20;5(1):89-99. PMID: 39872837
Curr Issues Mol Biol

Meningeal Lymphatics Drives Macrophage Clearance via CCL2-CCR2 Axis After Cerebral Ischemia. [Abstract]

2026 Feb 28;48(3):259. PMID: 41899411
IBRO Neurosci Rep

Proteoglycan 4 attenuates ischemic post-stroke hemorrhagic transformation and blood-brain barrier disruption. [Abstract]

2025 Sep 21:19:661-667. PMID: 41078397
J Biomed Opt

Multiscale imaging of colitis in mice using confocal laser endomicroscopy, light-sheet fluorescence microscopy, and magnetic resonance imaging. [Abstract]

2019 Jan;24(1):1-8. PMID: 30701723
Mol Cell Biol

miR-365-3p Regulates Gastrointestinal Dysfunction in Diabetes Mellitus Rats via the TLR4/MyD88/NF-κB Pathway. [Abstract]

2025 Jul 22:1-17. PMID: 40693422
Immun Inflamm Dis

Neohesperidin Dihydrochalcone Alleviates Lipopolysaccharide-Induced Vascular Endothelium Dysfunction by Regulating Antioxidant Capacity. [Abstract]

2024 Dec;12(12):e70107. PMID: 39699295
Exp Eye Res

Deletion of the Pedf gene leads to inflammation, photoreceptor loss and vascular disturbances in the retina. [Abstract]

2022 Sep:222:109171. PMID: 35809620
Virology

Direct blue 53, a biological dye, inhibits SARS-CoV-2 infection by blocking ACE2 and spike interaction in vitro and in vivo. [Abstract]

2023 Sep:586:105-114. PMID: 37531695
J Cardiovasc Dev Dis

2026 Jun 12;13(6):266. PMID: 42346486
BMC Neurosci

Esketamine attenuates traumatic brain injury by modulating STAT3-mediated Glycolysis and immune responses. [Abstract]

2025 Mar 7;26(1):21. PMID: 40055623
Physiol Res

Hirudin Alleviates Early Brain Injury After Subarachnoid Hemorrhage in Rats via Regulating NLRP3 Inflammasome-Mediated Pyroptosis. [Abstract]

2025 Apr 30;74(2):301-312. PMID: 40432444
Res Sq

2026 Jan 14.
Res Sq

2024 Jun 25.
bioRxiv

2024 May 13.

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : 31.25 mg/mL (32.52 mM; Need ultrasonic)

DMSO : ≥ 25 mg/mL (26.02 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.0408 mL

5.2039 mL

10.4079 mL

5 mM

0.2082 mL

1.0408 mL

2.0816 mL

10 mM

0.1041 mL

0.5204 mL

1.0408 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (2.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (2.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: 2.5 mg/mL (2.60 mM); Clear solution; Need warming

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (420 KB)
- English - EN (420 KB)
- Français - FR (420 KB)
- Deutsch - DE (420 KB)
- Norwegian - NO (420 KB)
- Español - ES (420 KB)
- Swedish - SV (420 KB)
- Italian - IT (420 KB)
- Korean - KR (420 KB)
- Portuguese - PT (420 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Linpeng Yao, et al. Evans Blue Dye: A Revisit of Its Applications in Biomedicine. Contrast Media Mol Imaging. 2018 Apr 22:2018:7628037. [Content Brief]

[2]. Anatol Manaenko, et al. Comparison Evans Blue injection routes: Intravenous versus intraperitoneal, for measurement of blood-brain barrier in a mice hemorrhage model. J Neurosci Methods. 2011 Feb 15;195(2):206-10. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	1.0408 mL	5.2039 mL	10.4079 mL	26.0197 mL
	5 mM	0.2082 mL	1.0408 mL	2.0816 mL	5.2039 mL
	10 mM	0.1041 mL	0.5204 mL	1.0408 mL	2.6020 mL
	15 mM	0.0694 mL	0.3469 mL	0.6939 mL	1.7346 mL
	20 mM	0.0520 mL	0.2602 mL	0.5204 mL	1.3010 mL
	25 mM	0.0416 mL	0.2082 mL	0.4163 mL	1.0408 mL
H₂O	30 mM	0.0347 mL	0.1735 mL	0.3469 mL	0.8673 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Evans Blue

31 Cited Publications

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Evans Blue

Membrane Transporter/Ion Channel Compound Library

Neuronal Signaling Compound Library

FDA-Approved Drug Library

Peptidomimetic Library

Drug Repurposing Compound Library

Covalent Screening Library

Anti-Cardiovascular Disease Compound Library

Neurotransmitter Receptor Compound Library

FDA Approved & Pharmacopeial Drug Library

Neuroprotective Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Antidepressant Compound Library

Off-patent Drug Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Ion Channel Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

Covalent Screening Library Plus

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

FDA-Approved Drug Library Mini

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) Evans Blue

Alle EAAT Isoform-spezifische Produkte anzeigen

Alle iGluR Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (31)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Evans Blue

Membrane Transporter/Ion Channel Compound Library

Neuronal Signaling Compound Library

FDA-Approved Drug Library

Peptidomimetic Library

Drug Repurposing Compound Library

Covalent Screening Library

Anti-Cardiovascular Disease Compound Library

Neurotransmitter Receptor Compound Library

FDA Approved & Pharmacopeial Drug Library

Neuroprotective Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Antidepressant Compound Library

Off-patent Drug Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Ion Channel Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

Covalent Screening Library Plus

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

FDA-Approved Drug Library Mini

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) Evans Blue

Alle EAAT Isoform-spezifische Produkte anzeigen

Alle iGluR Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (31)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Evans Blue Related Classifications

HY-B1102 Related Classifications