Art. -Nr.: HY-B0822

Fipronil

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Fipronil is a broad-spectrum insecticide effective against Lepidoptera species as well as thrips, locusts, ants, cockroaches, fleas and ticks. Fipronil selectively inhibits GABA receptor with IC₅₀s of 30 nM and 1600 nM for cockroach and rat GABA receptors, respectively. Glutamate-gated chloride channels (GluCls), which are present in cockroaches but not in mammals, are sensitive to the blocking effect of Fipronil. Fipronil also induces apoptosis in HepG2 cells and promotes the expression of CYP1A1 and CYP3A4 mRNA in human hepatocytes.

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Reinheit: 99.49%
CAS. Nr.: 120068-37-3
Formel: C₁₂H₄Cl₂F₆N₄OS
Molecular Weight:437.15

Speicherung:

4°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Fipronil Chemical Structure

CAS. Nr.: 120068-37-3

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

CYP1

CYP2

CYP3

Alle Cytochrome P450 Isoform-spezifische Produkte anzeigen :

In Vitro

Fipronil (0.1-25 µM; 72 h) dose-dependently induces expression of CYP1A1 and CYP3A4 mRNA in human hepatocytes^[1].
Fipronil (0.1-25 µM; 72 h) induces caspase-3/7 activity in a time- and dose-dependent manner, in HepG2 cells (caspase-3/7 activity is an important marker of the cellular apoptotic process)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:

Human hepatocytes
Concentration:

0.1-25 µM
Incubation Time:

72 h
Result:

Remarkably increased the expression of CYP1A1 and CYP3A4 mRNA in a dose-dependent manner.

Apoptosis Analysis^[1]

Cell Line:

HepG2 cells
Concentration:

0.05, 0.1, 0.5, 1, 3.125, 6.25, 12.5, 25, 50 µM
Incubation Time:

24, 48, 72 h
Result:

Exhibited time- and dose-dependent induction of caspase-3/7 activity from 0.1 to 6.25 µM, while slowly decreasing from 12.5 to 50 µM.

In Vivo

Fipronil inhibits two types of glutamate-induced chloride desensitizing and non-desensitizing current with IC₅₀ values of 800 and 10 nM, respectively^[2].
Fipronil is more selective for cockroach GABA receptors than for rat GABA^[2].
Fipronil blocks cockroach GABA receptors in both resting and activated states, with IC₅₀s of 28 and 35 nM, respectively^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 120068-37-3
Appearance Solid
Molecular Weight 437.15
Formel C₁₂H₄Cl₂F₆N₄OS
Color White to off-white
SMILES

N#CC1=NN(C2=C(Cl)C=C(C(F)(F)F)C=C2Cl)C(N)=C1S(C(F)(F)F)=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 250 mg/mL (571.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2875 mL

11.4377 mL

22.8754 mL

5 mM

0.4575 mL

2.2875 mL

4.5751 mL

10 mM

0.2288 mL

1.1438 mL

2.2875 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.76 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.76 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
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COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Das PC, et al. Fipronil induces CYP isoforms and cytotoxicity in human hepatocytes. Chem Biol Interact. 2006 Dec 15;164(3):200-14. [Content Brief]

[2]. Narahashi T, et al. Glutamate-activated chloride channels: Unique fipronil targets present in insects but not in mammals. Pestic Biochem Physiol. 2010 Jun 1;97(2):149-152. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2875 mL	11.4377 mL	22.8754 mL	57.1886 mL
	5 mM	0.4575 mL	2.2875 mL	4.5751 mL	11.4377 mL
	10 mM	0.2288 mL	1.1438 mL	2.2875 mL	5.7189 mL
	15 mM	0.1525 mL	0.7625 mL	1.5250 mL	3.8126 mL
	20 mM	0.1144 mL	0.5719 mL	1.1438 mL	2.8594 mL
	25 mM	0.0915 mL	0.4575 mL	0.9150 mL	2.2875 mL
	30 mM	0.0763 mL	0.3813 mL	0.7625 mL	1.9063 mL
	40 mM	0.0572 mL	0.2859 mL	0.5719 mL	1.4297 mL
	50 mM	0.0458 mL	0.2288 mL	0.4575 mL	1.1438 mL
	60 mM	0.0381 mL	0.1906 mL	0.3813 mL	0.9531 mL
	80 mM	0.0286 mL	0.1430 mL	0.2859 mL	0.7149 mL
	100 mM	0.0229 mL	0.1144 mL	0.2288 mL	0.5719 mL

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Fipronil

Anti-Infection Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Membrane Transporter/Ion Channel Compound Library

Metabolism/Protease Compound Library

Neuronal Signaling Compound Library

FDA-Approved Drug Library

Anti-Cancer Compound Library

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Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

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FDA-Approved Anticancer Drug Library

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FDA-Approved Drug Library Plus

FDA-Approved Drug Library Mini

Bioactive Compound Library

Pesticide Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Environmental Pollutant Compound Library

Classic FDA-Approved Drug Library

Alle Cytochrome P450 Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Fipronil Related Classifications

HY-B0822 Related Classifications