Cat. No.: HY-N2179

Hypaphorine

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Hypaphorine is an indole found in Caragana korshinskii. Hypaphorine has neurological and glucose-lowering effects. Hypaphorine prevents Lipopolysaccharides (LPS) (HY-D1056)-mediated acute lung injury (ALI) and proinflammatory response via inactivating the p38/JNK signaling pathway by upregulating DUSP1.

For research use only. We do not sell to patients.

Purity: 99.93%
CAS No.: 487-58-1
Formula: C₁₄H₁₈N₂O₂
Molecular Weight:246.31

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Hypaphorine Chemical Structure

CAS No.: 487-58-1

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All p38 MAPK Isoforms :

All JNK Isoforms :

In Vitro

Hypaphorine (6.25-100 μM, 24 h) inhibits the Lipopolysaccharides (LPS) (HY-D1056)-mediated apoptosis of alveolar epithelial cells^[2].
Hypaphorine (12.5-50 μM, 48 h) attenuates the expression of inflammatory factors (IL-1β, IL-6, TNF-α, and IL-18) and inactivates the p38/JNK signaling pathway through up-regulating DUSP1 in a dose-dependent manner^[2].
Hypaphorine reverses inhibition of E. globulus seedling root growth induced by 3-Indoleacetic acid (Indole-3-acetic acid) (HY-18569)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[2]

Cell Line:

BEAS-2B lung epithelial cells
Concentration:

6.25, 12.5, 25, 50, 100 μM
Incubation Time:

24 h
Result:

Cell viability was not changed when the dose was less than 50 μM, cell viability decreased significantly at 100 μM.
Cell viability was elevated dose-dependently (12.5, 25, 50 μM) in an ALI model.
The expression of pro-apoptotic proteins Bax and Caspase3 was dampened, while the anti-apoptotic protein Bcl2 was increased significantly in a dose-dependent manner.

RT-PCR^[2]

Cell Line:

BEAS-2B lung epithelial cells
Concentration:

12.5, 25, 50 μM
Incubation Time:

48 h
Result:

Promoted the level of DUSP1 mRNA in dose-dependent manner.
DUSP1 was significantly upregulated after LPS treatment.

Western Blot Analysis^[2]

Cell Line:

BEAS-2B lung epithelial cells
Concentration:

12.5, 25, 50 μM
Incubation Time:

48 h
Result:

Distinctly strengthened the DUSP1 expression and inactivated p38/JNK pathway.
Enhanced DUSP1 protein level and inactivated the p38/JNK signaling pathway after LPS treatment.

In Vivo

Hypaphorine (10 mg/kg; i.p.; 2 hours after the LPS injection) relieves LPS induced acute lung injury (ALI) in rats with anti-inflammatory effects^[2].
Hypaphorine (50 mg/kg; i.p.; once) has antihyperglycemic activity^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male albino rats of Wistar/Sprague-Dawley strain (8-10 weeks, 160 g) made diabetic by intraperitoneal injection of Streptozotocin (HY-13753)^[4]
Dosage:

50 mg/kg
Administration:

Intraperitoneal injection (i.p.); once
Result:

Showed significant anti-hyperglycemic activity (27.8% at 5 h and 20.4% at 24 h).

Animal Model:

Male Sprague-Dawley rats (200-220 g) treated with LPS^[2]
Dosage:

10 mg/kg
Administration:

Intraperitoneal injection (i.p.); 2 hours after the LPS injection
Result:

Alleviated the histological changes in lung tissues induced by LPS, including severe inflammatory cell infiltration, thickened alveolar walls, obvious diffuse edema, narrowed alveolar space, strengthened interstitial congestion, serious pathological damage and higher scores.
Reduced BALF cells and neutrophils increased by LPS.
Attenuated the wet/dry weight ratio, capillary permeability and d myeloperoxidase (MPO) activity facilitated by LPS.

Chemical Information

CAS No. 487-58-1
Appearance Solid
Molecular Weight 246.31
Formula C₁₄H₁₈N₂O₂
Color White to off-white
SMILES

C[N+](C)(C)[C@H](C([O-])=O)CC1=CNC2=CC=CC=C12
Structure Classification
- Alkaloids
- Indole Alkaloids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (2)

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Journal Impact Factor

Kaohsiung J Med Sci

Hypaphorine exerts anti-inflammatory effects in sepsis induced acute lung injury via modulating DUSP1/p38/JNK pathway. [Abstract]

2021 Oct;37(10):883-893. PMID: 34250720
SSRN

2025 Nov 11.

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (405.99 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.0599 mL

20.2996 mL

40.5992 mL

5 mM

0.8120 mL

4.0599 mL

8.1198 mL

10 mM

0.4060 mL

2.0300 mL

4.0599 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

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Molecular Weight Molecular Weight

Dilution Calculator

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In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (405.99 mM); Clear solution; Need ultrasonic

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Keller BO, et al. Hypaphorine is present in human milk in association with consumption of legumes. J Agric Food Chem. 2013 Aug 7;61(31):7654-60. [Content Brief]

[2]. Ding YH, et al. Hypaphorine exerts anti-inflammatory effects in sepsis induced acute lung injury via modulating DUSP1/p38/JNK pathway. Kaohsiung J Med Sci. 2021 Oct;37(10):883-893. [Content Brief]

[3]. Ditengou FA, et al. Hypaphorine from the ectomycorrhizal fungus Pisolithus tinctorius counteracts activities of indole-3-acetic acid and ethylene but not synthetic auxins in eucalypt seedlings. Mol Plant Microbe Interact. 2000 Feb;13(2):151-8. [Content Brief]

[4]. Chand K, et al. Major alkaloidal constituent from Impatiens niamniamensis seeds as antihyperglycemic agent. Med Chem Res 20, 1505–1508 (2011).

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	4.0599 mL	20.2996 mL	40.5992 mL	101.4981 mL
	5 mM	0.8120 mL	4.0599 mL	8.1198 mL	20.2996 mL
	10 mM	0.4060 mL	2.0300 mL	4.0599 mL	10.1498 mL
	15 mM	0.2707 mL	1.3533 mL	2.7066 mL	6.7665 mL
	20 mM	0.2030 mL	1.0150 mL	2.0300 mL	5.0749 mL
	25 mM	0.1624 mL	0.8120 mL	1.6240 mL	4.0599 mL
	30 mM	0.1353 mL	0.6767 mL	1.3533 mL	3.3833 mL
	40 mM	0.1015 mL	0.5075 mL	1.0150 mL	2.5375 mL
	50 mM	0.0812 mL	0.4060 mL	0.8120 mL	2.0300 mL
	60 mM	0.0677 mL	0.3383 mL	0.6767 mL	1.6916 mL
	80 mM	0.0507 mL	0.2537 mL	0.5075 mL	1.2687 mL
	100 mM	0.0406 mL	0.2030 mL	0.4060 mL	1.0150 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Hypaphorine

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Publications Citing Use of MedChemExpress (MCE) Hypaphorine

All p38 MAPK Isoforms

All JNK Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Hypaphorine Related Classifications

HY-N2179 Related Classifications