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Cat. No.: HY-N7151R

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Aurachin D (Standard)

Aurachin D (Standard) is the analytical standard of Aurachin D (HY-N7151). This product is intended for research and analytical applications. Aurachin D is an antibiotic and selective cytochrome bd oxidase inhibitor. Aurachin D exhibits inhibitory effects against Gram-positive bacteria and a small number of fungi, with an IC₅₀ value of 0.1 μM against Escherichia coli and an IC₅₀ value ranging from 0.13 to 33 μM against Mycobacterium tuberculosis. Aurachin D binds to and inhibits cytochrome bd oxidase, thereby blocking the electron transfer process, disrupting the respiratory chain and energy metabolism of pathogens. Aurachin D can be used in research related to leishmaniasis, malaria, Chagas disease, African trypanosomiasis, and tuberculosis [2].

For research use only. We do not sell to patients.

CAS No.: 108354-13-8
Formula: C₂₅H₃₃NO
Molecular Weight:363.54

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (269 KB)

Aurachin D (Standard) Chemical Structure

CAS No.: 108354-13-8

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
Chemical Information
Protocols (2)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

All Antibiotic Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
L929	IC₅₀	1.3 μg/mL Compound: 3	Cytotoxicity against mouse L929 cells after 5 days by MTT assay Cytotoxicity against mouse L929 cells after 5 days by MTT assay	[PMID: 18922036]

Chemical Information

CAS No. 108354-13-8
Molecular Weight 363.54
Formula C₂₅H₃₃NO
SMILES

O=C1C(C/C=C(C)/CC/C=C(C)/CC/C=C(C)\C)=C(C)NC2=C1C=CC=C2
Structure Classification
- Alkaloids
- Quinoline Alkaloids
Initial Source
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology Histomorphology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kunze B, et al. The aurachins, new quinoline antibiotics from myxobacteria: production, physico-chemical and biological properties. The Journal of antibiotics. 1987 Mar;40(3):258-65. [Content Brief]

[2]. Kruth S, et al. Aurachins, Bacterial Antibiotics Interfering with Electron Transport Processes. Antibiotics (Basel, Switzerland). 2023 Jun 17;12(6):1067.

[3]. Hasenoehrl EJ,et al. Bioenergetic Inhibitors: Antibiotic Efficacy and Mechanisms of Action in Mycobacterium tuberculosis. Front Cell Infect Microbiol. 2021 Jan 11;10:611683.

[4]. Lawer A, et al. Synthesis and Biological Evaluation of Aurachin D Analogues as Inhibitors of Mycobacterium tuberculosis Cytochrome bd Oxidase. ACS Med Chem Lett. 2022 Sep 26;13(10):1663-1669.

[5]. Kruth S, et al. Generation of Aurachin Derivatives by Whole-Cell Biotransformation and Evaluation of Their Antiprotozoal Properties. Molecules (Basel, Switzerland). 2023 Jan 20;28(3):1066.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Minimum order quantity

100 mg

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