2-Aminobenzothiazole
Based on 1 Customer Validation
2-Aminobenzothiazole acts as a caspase 3/7 activator, an anticancer cytotoxic agent, and also exhibits neurotoxicity. 2-Aminobenzothiazole drives the apoptotic pathway by activating caspase 3/7, induces mitochondrial inner membrane depolarization, and triggers both early and late apoptosis via a caspase-dependent pathway. In zebrafish models, 2-Aminobenzothiazole induces oxidative damage in brain tissues and inhibits genes related to GABA and 5-HT synthesis pathways. Long-term exposure to 2-Aminobenzothiazole impairs motor ability, social behavior, anxiety-like state and cognitive function. 2-Aminobenzothiazole can be used in studies of human laryngeal carcinoma and related neurotoxicity.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 136-95-8
- Formula: C7H6N2S
- Molecular Weight:150.21
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Storage:
RT, protect from light.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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All Caspase Isoforms
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Biological Activity
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Caspase 3 |
Caspase-7 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO | IC50 |
>100 μM
Compound: 7
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Inhibition of human VAP-1 expressed in CHO cells using [14C]-benzylamine as substrate preincubated for 30 mins prior to substrate addition measured after 1 hr by scintillation spectrometric analysis
Inhibition of human VAP-1 expressed in CHO cells using [14C]-benzylamine as substrate preincubated for 30 mins prior to substrate addition measured after 1 hr by scintillation spectrometric analysis
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[PMID: 23664164] |
| CHO | IC50 |
>100 μM
Compound: 7
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Inhibition of rat VAP-1 expressed in CHO cells using [14C]-benzylamine as substrate preincubated for 30 mins prior to substrate addition measured after 1 hr by scintillation spectrometric analysis
Inhibition of rat VAP-1 expressed in CHO cells using [14C]-benzylamine as substrate preincubated for 30 mins prior to substrate addition measured after 1 hr by scintillation spectrometric analysis
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[PMID: 23664164] |
2-aminobenzothiazole (3.125-100 μM; 24-72 h) exerts dose-dependent cytotoxicity against human larynx epidermoid carcinoma (HEp-2) cells, with IC50 values of 5 μM (24 h), 27 μM (48 h), and 5 μM (72 h)[1].
2-aminobenzothiazole (IC50 concentration; 24 h) induces apoptosis in human larynx epidermoid carcinoma (HEp-2) cells, with 42.62% of cells undergoing early or late apoptosis after 24 hours[1].
2-aminobenzothiazole (IC50 concentration; 24 h) activates caspase 3/7 in human larynx epidermoid carcinoma (HEp-2) cells[1].
2-Aminobenzothiazole inhibits the activity of porcine thyroid peroxidase, with intermediate potency relative to other benzothiazole derivatives[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human larynx epidermoid carcinoma (HEp-2) cells
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Concentration:3.125-100 μM (24 h); 3.13-100 μM (48 h); 3.13-100 μM (72 h)
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Incubation Time:24 h; 48 h; 72 h
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Result:Caused a statistically significant (p < 0.05) decrease in HEp-2 cell viability at 3.125, 6.25, 12.5, 25, 50, 100 μM after 24 h incubation, with the highest growth inhibition at 100 μM, and an IC50 of 5 μM.
Caused a statistically significant (p < 0.05) decrease in HEp-2 cell viability at 6.25, 12.5, 25, 50, 100 μM after 48 h incubation, with the highest cytotoxicity at 100 μM, and an IC50 of 27 μM.
Induced a statistically significant (p < 0.05) viability reduction at 12.5, 25, 50, 100 μM after 72 h incubation, with the highest cytotoxicity at 100 μM, and an IC50 of 5 μM.
Exerted a dose-dependent cytotoxic effect across all incubation times.
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Cell Line:human larynx epidermoid carcinoma (HEp-2) cells
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Concentration:IC50 concentration (24 h)
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Incubation Time:24 h
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Result:Resulted in 56.93% of HEp-2 cells being alive, 25.99% in early apoptosis, 16.69% in late apoptosis, and 0.39% necrotic, with a total apoptotic cell percentage of 42.62% after 24-hour exposure.
2-Aminobenzothiazole (50-500 μg/L; aqueous exposure; water renewal every two days; 120 dpf) induces significant neurobehavioral deficits (reduced locomotion, anxiety-like behavior, impaired memory), oxidative brain damage, and dysregulation of GABA and 5-HT pathway gene expression in adult Danio rerio[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wild-type AB (adults, 120 days post-fertilization [dpf])[2]
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Dosage:50 μg/L; 500 μg/L
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Administration:aqueous exposure; water renewal every two days; 120 dpf
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Result:Caused a significant 17.6% decrease in adult zebrafish body weight and 5.1% decrease in body length (500 μg/L, P < 0.05).
Showed no significant effect on adult zebrafish body weight or length (50 μg/L).
Significantly reduced adult zebrafish swimming velocity (50 and 500 μg/L, P < 0.05).
Significantly decreased entries into the center zone, increased distance between individuals, and reduced body contacts in adult zebrafish (500 μg/L, P < 0.05).
Significantly reduced time spent in the tank top and increased immobility time in the tank bottom in adult zebrafish (50 and 500 μg/L, P < 0.05).
Significantly reduced movement speed in the novel object area, decreased time spent in the novel object area, and increased time spent in the familiar object area in adult zebrafish (50 and 500 μg/L, P < 0.05).
Significantly increased brain tissue CAT and GSH enzyme activities, elevated MDA content, and inhibited SOD enzyme activity in adult zebrafish (50 and 500 μg/L, P < 0.05).
Significantly upregulated brain expression of oxidative stress-related genes (Cat, Mn-sod, nrf2, Cu/Zn-sod) in adult zebrafish (50 and 500 μg/L, P < 0.05).
Significantly suppressed expression of GABA synthesis pathway genes (gabrg2, gad2, gad1b, abat) and 5-HT synthesis pathway genes (tph2, tph1b, pet1, htr1aa) in adult zebrafish brain (50 and 500 μg/L, P < 0.05).
Chemical Information
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CAS No. 136-95-8
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Appearance Solid
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Molecular Weight 150.21
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Formula C7H6N2S
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Color White to light yellow
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SMILES
NC1=NC2=CC=CC=C2S1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, protect from light
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
DMSO : 100 mg/mL (665.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.64 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (16.64 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Haznedar B, et al. Investigation of proapoptotic and cytotoxic effects of 2-aminobenzothiazole on human laryngeal carcinoma cells. Eur Rev Med Pharmacol Sci. 2024;28(4):1585-1593. [Content Brief]
[2]. Gu J, et al. Neurobehavioral toxic effects and mechanisms of 2-aminobenzothiazole exposure on zebrafish. Sci Total Environ. 2024;913:169495. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.6573 mL | 33.2867 mL | 66.5735 mL | 166.4337 mL |
| 5 mM | 1.3315 mL | 6.6573 mL | 13.3147 mL | 33.2867 mL | |
| 10 mM | 0.6657 mL | 3.3287 mL | 6.6573 mL | 16.6434 mL | |
| 15 mM | 0.4438 mL | 2.2191 mL | 4.4382 mL | 11.0956 mL | |
| 20 mM | 0.3329 mL | 1.6643 mL | 3.3287 mL | 8.3217 mL | |
| 25 mM | 0.2663 mL | 1.3315 mL | 2.6629 mL | 6.6573 mL | |
| 30 mM | 0.2219 mL | 1.1096 mL | 2.2191 mL | 5.5478 mL | |
| 40 mM | 0.1664 mL | 0.8322 mL | 1.6643 mL | 4.1608 mL | |
| 50 mM | 0.1331 mL | 0.6657 mL | 1.3315 mL | 3.3287 mL | |
| 60 mM | 0.1110 mL | 0.5548 mL | 1.1096 mL | 2.7739 mL | |
| 80 mM | 0.0832 mL | 0.4161 mL | 0.8322 mL | 2.0804 mL | |
| 100 mM | 0.0666 mL | 0.3329 mL | 0.6657 mL | 1.6643 mL |
2-Aminobenzothiazole Related Classifications
HY-W017424 Related Classifications
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