BMS-587101
Based on 1 Customer Validation
BMS-587101 is a potent and orally active antagonist of leukocyte function associated antigen-1 (LFA-1). BMS-587101 has anti-inflammatory effects and can be used for rheumatoid arthritis research.
For research use only. We do not sell to patients.
- Purity: 99.39%
- CAS No.: 509083-77-6
- Formula: C26H20Cl2N4O4S
- Molecular Weight:555.43
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| bEND | IC50 |
150 nM
Compound: 8, BMS-587101
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Inhibition of mouse bEND cell adhesion to T cell
Inhibition of mouse bEND cell adhesion to T cell
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[PMID: 17125246] |
| HUVEC | IC50 |
18 nM
Compound: 1, BMS-688521
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Inhibition of LFA1-mediated adhesion of human T cells to ICAM1-expressing HUVEC by fluorescence assay
Inhibition of LFA1-mediated adhesion of human T cells to ICAM1-expressing HUVEC by fluorescence assay
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[PMID: 20405922] |
| HUVEC | IC50 |
20 nM
Compound: 8, BMS-587101
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Inhibition of LFA1-mediated adhesion of T cell to HUVEC
Inhibition of LFA1-mediated adhesion of T cell to HUVEC
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[PMID: 17125246] |
| PBMC | IC50 |
280 nM
Compound: 8, BMS-587101
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Antiproliferative activity against PBMC by MLR assay
Antiproliferative activity against PBMC by MLR assay
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[PMID: 17125246] |
| T-cell | IC50 |
150 nM
Compound: 1, BMS-688521
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Inhibition of LFA1-mediated adhesion of C57BL/6 mouse T cells to ICAM1-expressing TNF-alpha-activated mouse b.END3 cells by calcein-AM staining-based fluorescence assay
Inhibition of LFA1-mediated adhesion of C57BL/6 mouse T cells to ICAM1-expressing TNF-alpha-activated mouse b.END3 cells by calcein-AM staining-based fluorescence assay
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[PMID: 20405922] |
| T-cell | IC50 |
280 nM
Compound: 1, BMS-688521
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Inhibition of LFA1-mediated human T cell proliferation assessed as [3H]thymidine uptake after 4 days by mixed lymphocyte reaction assay
Inhibition of LFA1-mediated human T cell proliferation assessed as [3H]thymidine uptake after 4 days by mixed lymphocyte reaction assay
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[PMID: 20405922] |
BMS-587101 is a moderately potent inhibitor of LFA-1-mediated T-cell proliferation, with an IC50 of 20 nM in human HUVEC cells. BMS-587101 inhibits LFA-1-mediated adhesion of T cells to endothelial cells, T cell proliferation, and Th1 cytokine production[1][2].
?
In mouse splenocytes and a mouse ICAM-1 expressing cell lines, bEND, BMS-587101 shows inhibitory activities, with an IC50 of 150 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 509083-77-6
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Appearance Solid
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Molecular Weight 555.43
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Formula C26H20Cl2N4O4S
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Color Off-white to light yellow
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SMILES
O=C(C1=CSC(CN(C[C@H]2C3=CC=C(C#N)C=C3)C[C@]2(N(C)C(N4C5=CC(Cl)=CC(Cl)=C5)=O)C4=O)=C1)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 260 mg/mL (468.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Dominique Potin, et al. Discovery and development of 5-[(5S,9R)-9-(4-cyanophenyl)-3-(3,5-dichlorophenyl)-1-methyl-2,4-dioxo-1,3,7-triazaspiro[4.4]non-7-yl-methyl]-3-thiophenecarboxylic acid (BMS-587101)--a small molecule antagonist of leukocyte function a [Content Brief]
[2]. Suzanne J Suchard, et al. An LFA-1 (alphaLbeta2) small-molecule antagonist reduces inflammation and joint destruction in murine models of arthritis. J Immunol. 2010 Apr 1;184(7):3917-26. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8004 mL | 9.0020 mL | 18.0041 mL | 45.0102 mL |
| 5 mM | 0.3601 mL | 1.8004 mL | 3.6008 mL | 9.0020 mL | |
| 10 mM | 0.1800 mL | 0.9002 mL | 1.8004 mL | 4.5010 mL | |
| 15 mM | 0.1200 mL | 0.6001 mL | 1.2003 mL | 3.0007 mL | |
| 20 mM | 0.0900 mL | 0.4501 mL | 0.9002 mL | 2.2505 mL | |
| 25 mM | 0.0720 mL | 0.3601 mL | 0.7202 mL | 1.8004 mL | |
| 30 mM | 0.0600 mL | 0.3001 mL | 0.6001 mL | 1.5003 mL | |
| 40 mM | 0.0450 mL | 0.2251 mL | 0.4501 mL | 1.1253 mL | |
| 50 mM | 0.0360 mL | 0.1800 mL | 0.3601 mL | 0.9002 mL | |
| 60 mM | 0.0300 mL | 0.1500 mL | 0.3001 mL | 0.7502 mL | |
| 80 mM | 0.0225 mL | 0.1125 mL | 0.2251 mL | 0.5626 mL | |
| 100 mM | 0.0180 mL | 0.0900 mL | 0.1800 mL | 0.4501 mL |
BMS-587101 Related Classifications
HY-120628 Related Classifications
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