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Cat. No.: HY-174866

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BT-O2C

Customer Review

Based on 1 Customer Validation

BT-O2C is a highly selective p300 PROTAC degrader. BT-O2C can significantly degrade the level of p300 in HAP1 cells. BT-O2C has significant cytotoxicity in CIC:DUX4 sarcoma (CDS) cell lines (IC₅₀ ranges from 152 to 221 nM) and significantly reduces the expression of CDS target genes (ETV1, ETV4, ETV5). BT-O2C can be used for research on cancer.
(Pink: p300 ligand (HY-174868); Blue: Cereblon ligand (HY-W023573); Black: linker).

For research use only. We do not sell to patients.

Purity: 99.31%
CAS No.: 3055107-34-8
Formula: C₄₈H₄₈F₅N₉O₈
Molecular Weight:973.94

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

BT-O2C Chemical Structure

CAS No.: 3055107-34-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

BT-O2C is a highly selective p300 PROTAC degrader. BT-O2C can significantly degrade the level of p300 in HAP1 cells. BT-O2C has significant cytotoxicity in CIC:DUX4 sarcoma (CDS) cell lines (IC₅₀ ranges from 152 to 221 nM) and significantly reduces the expression of CDS target genes (ETV1, ETV4, ETV5). BT-O2C can be used for research on cancer^[1]. (Pink: p300 ligand (HY-174868); Blue: Cereblon ligand (HY-W023573); Black: linker).

IC₅₀ & Target^[1]

p300

Cereblon

All Histone Acetyltransferase Isoforms :

All Epigenetic Reader Domain Isoforms :

All PROTACs Isoforms :

In Vitro

BT-O2C (0.1-10 μM, 24 h) can significantly degrade p300 and has almost no effect on the degradation of CBP in HAPI cells^[1].
BT-O2C (0.25-4 μM, 48-72 h) exhibits significant cytotoxicity in NCC-CDS1-X1 (IC₅₀ = 152 nM) and KITRA (IC₅₀ = 221 nM) cells^[1].
BT-O2C (4 μM, 6 h) significantly reduces the expression of CDS target genes in NCC-CDS-X1 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

HAPI cells
Concentration:

0.1, 1 and 10 μM
Incubation Time:

24 h
Result:

Selectively degraded the protein level of p300, superior to JQAD1 (HY-145765).

RT-PCR^[1]

Cell Line:

NCC-CDS-X1 cells
Concentration:

4 μM
Incubation Time:

6 h
Result:

Significantly reduced the expression of ETV1, ETV4, ETV5, CCNEE1 and MYC.

Chemical Information

CAS No. 3055107-34-8
Appearance Solid
Molecular Weight 973.94
Formula C₄₈H₄₈F₅N₉O₈
Color Light yellow to yellow
SMILES

O=C(N(CC1=CC=C(F)C=C1)[C@@H](C)C(F)(F)F)CN(C(N[C@]23C[C@@H](F)C4=C3C=CC(C5=CN(CC(NCCCCCCCNC6=CC7=C(C(N(C(CC8)C(NC8=O)=O)C7=O)=O)C=C6)=O)N=C5)=C4)=O)C2=O
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (268 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Marsh GP, et al. Development of p300-targeting degraders with enhanced selectivity and onset of degradation. RSC Med Chem. 2025 Mar 3;16(5):2049-2060. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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BT-O2C

PROTAC Library

MCE Bioactive Compound Library

All Histone Acetyltransferase Isoforms

All Epigenetic Reader Domain Isoforms

All PROTACs Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

BT-O2C

PROTAC Library

MCE Bioactive Compound Library

All Histone Acetyltransferase Isoforms

All Epigenetic Reader Domain Isoforms

All PROTACs Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

BT-O2C Related Classifications

HY-174866 Related Classifications