Art. -Nr.: HY-174866

BT-O2C

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BT-O2C is a highly selective p300 PROTAC degrader. BT-O2C can significantly degrade the level of p300 in HAP1 cells. BT-O2C has significant cytotoxicity in CIC:DUX4 sarcoma (CDS) cell lines (IC₅₀ ranges from 152 to 221 nM) and significantly reduces the expression of CDS target genes (ETV1, ETV4, ETV5). BT-O2C can be used for research on cancer.
(Pink: p300 ligand (HY-174868); Blue: Cereblon ligand (HY-W023573); Black: linker).

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Reinheit: 99.31%
CAS. Nr.: 3055107-34-8
Formel: C₄₈H₄₈F₅N₉O₈
Molecular Weight:973.94

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

BT-O2C Chemical Structure

CAS. Nr.: 3055107-34-8

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

BT-O2C is a highly selective p300 PROTAC degrader. BT-O2C can significantly degrade the level of p300 in HAP1 cells. BT-O2C has significant cytotoxicity in CIC:DUX4 sarcoma (CDS) cell lines (IC₅₀ ranges from 152 to 221 nM) and significantly reduces the expression of CDS target genes (ETV1, ETV4, ETV5). BT-O2C can be used for research on cancer^[1]. (Pink: p300 ligand (HY-174868); Blue: Cereblon ligand (HY-W023573); Black: linker).

IC₅₀ & Target^[1]

p300

Cereblon

Alle Histone Acetyltransferase Isoform-spezifische Produkte anzeigen :

Alle Epigenetic Reader Domain Isoform-spezifische Produkte anzeigen :

Alle PROTACs Isoform-spezifische Produkte anzeigen :

In Vitro

BT-O2C (0.1-10 μM, 24 h) can significantly degrade p300 and has almost no effect on the degradation of CBP in HAPI cells^[1].
BT-O2C (0.25-4 μM, 48-72 h) exhibits significant cytotoxicity in NCC-CDS1-X1 (IC₅₀ = 152 nM) and KITRA (IC₅₀ = 221 nM) cells^[1].
BT-O2C (4 μM, 6 h) significantly reduces the expression of CDS target genes in NCC-CDS-X1 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

HAPI cells
Concentration:

0.1, 1 and 10 μM
Incubation Time:

24 h
Result:

Selectively degraded the protein level of p300, superior to JQAD1 (HY-145765).

RT-PCR^[1]

Cell Line:

NCC-CDS-X1 cells
Concentration:

4 μM
Incubation Time:

6 h
Result:

Significantly reduced the expression of ETV1, ETV4, ETV5, CCNEE1 and MYC.

Chemical Information

CAS. Nr. 3055107-34-8
Appearance Solid
Molecular Weight 973.94
Formel C₄₈H₄₈F₅N₉O₈
Color Light yellow to yellow
SMILES

O=C(N(CC1=CC=C(F)C=C1)[C@@H](C)C(F)(F)F)CN(C(N[C@]23C[C@@H](F)C4=C3C=CC(C5=CN(CC(NCCCCCCCNC6=CC7=C(C(N(C(CC8)C(NC8=O)=O)C7=O)=O)C=C6)=O)N=C5)=C4)=O)C2=O
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (102.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.0268 mL

5.1338 mL

10.2676 mL

5 mM

0.2054 mL

1.0268 mL

2.0535 mL

10 mM

0.1027 mL

0.5134 mL

1.0268 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (2.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Marsh GP, et al. Development of p300-targeting degraders with enhanced selectivity and onset of degradation. RSC Med Chem. 2025 Mar 3;16(5):2049-2060. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.0268 mL	5.1338 mL	10.2676 mL	25.6689 mL
	5 mM	0.2054 mL	1.0268 mL	2.0535 mL	5.1338 mL
	10 mM	0.1027 mL	0.5134 mL	1.0268 mL	2.5669 mL
	15 mM	0.0685 mL	0.3423 mL	0.6845 mL	1.7113 mL
	20 mM	0.0513 mL	0.2567 mL	0.5134 mL	1.2834 mL
	25 mM	0.0411 mL	0.2054 mL	0.4107 mL	1.0268 mL
	30 mM	0.0342 mL	0.1711 mL	0.3423 mL	0.8556 mL
	40 mM	0.0257 mL	0.1283 mL	0.2567 mL	0.6417 mL
	50 mM	0.0205 mL	0.1027 mL	0.2054 mL	0.5134 mL
	60 mM	0.0171 mL	0.0856 mL	0.1711 mL	0.4278 mL
	80 mM	0.0128 mL	0.0642 mL	0.1283 mL	0.3209 mL
	100 mM	0.0103 mL	0.0513 mL	0.1027 mL	0.2567 mL

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BT-O2C

Alle Histone Acetyltransferase Isoform-spezifische Produkte anzeigen

Alle Epigenetic Reader Domain Isoform-spezifische Produkte anzeigen

Alle PROTACs Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

BT-O2C Related Classifications

HY-174866 Related Classifications