(5-Cl)-Exatecan
(5-Cl)-Exatecan is a derivative of Exatecan (HY-13631) and a DNA topoisomerase inhibitor. (5-Cl)-Exatecan serves as the cytotoxic payload component in antibody-drug conjugates (ADC) targeting ROR1-positive cancers. (5-Cl)-Exatecan is applicable for the research of ROR1-positive cancers.
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- CAS No.: 2813268-66-3
- Formule: C24H22ClN3O4
- Masse moléculaire:451.90
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
IC50 & Target
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Camptothecins |
In Vitro
(5-Cl)-Exatecan (1-5) can produce ADC compounds that have good targeted killing effects on colon cancer and non-small cell lung cancer (e.g., lung adenocarcinoma)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2813268-66-3
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Masse moléculaire 451.90
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Formule C24H22ClN3O4
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SMILES
N[C@@H]1C2=C3C(C(N4C3)=CC([C@](O)(C(OC5)=O)CC)=C5C4=O)=NC6=CC(Cl)=C(C)C(CC1)=C62
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvant et solubilité
In Vitro:
DMSO : ≥ 50 mg/mL (110.64 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureté et documentation
Références
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2129 mL | 11.0644 mL | 22.1288 mL | 55.3220 mL |
| 5 mM | 0.4426 mL | 2.2129 mL | 4.4258 mL | 11.0644 mL | |
| 10 mM | 0.2213 mL | 1.1064 mL | 2.2129 mL | 5.5322 mL | |
| 15 mM | 0.1475 mL | 0.7376 mL | 1.4753 mL | 3.6881 mL | |
| 20 mM | 0.1106 mL | 0.5532 mL | 1.1064 mL | 2.7661 mL | |
| 25 mM | 0.0885 mL | 0.4426 mL | 0.8852 mL | 2.2129 mL | |
| 30 mM | 0.0738 mL | 0.3688 mL | 0.7376 mL | 1.8441 mL | |
| 40 mM | 0.0553 mL | 0.2766 mL | 0.5532 mL | 1.3830 mL | |
| 50 mM | 0.0443 mL | 0.2213 mL | 0.4426 mL | 1.1064 mL | |
| 60 mM | 0.0369 mL | 0.1844 mL | 0.3688 mL | 0.9220 mL | |
| 80 mM | 0.0277 mL | 0.1383 mL | 0.2766 mL | 0.6915 mL | |
| 100 mM | 0.0221 mL | 0.1106 mL | 0.2213 mL | 0.5532 mL |
(5-Cl)-Exatecan Related Classifications
HY-160806 Related Classifications
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