Cat. No.: HY-N0038

Alantolactone

(Synonyms: (+)-Alantolactone; Alant camphor; Inula camphor)

10 Cited Publications

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Based on 10 publication(s) in Google Scholar

Alantolactone is a selective STAT3 inhibitor, with potent anticancer activity. Alantolactone induces apoptosis in cancer.

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Pureté: 99.94%
CAS No.: 546-43-0
Formule: C₁₅H₂₀O₂
Masse moléculaire:232.32

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Alantolactone Chemical Structure

CAS No.: 546-43-0

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (10)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Alantolactone is a selective STAT3 inhibitor, with potent anticancer activity. Alantolactone induces apoptosis in cancer^[1]^[2]^[3].

IC₅₀ & Target

STAT3

Voir tous les produits spécifiques à Isoform STAT :

Voir tous les produits spécifiques à Isoform TGF-beta/Smad :

Smad1
Smad3

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.55 μg/mL Compound: 6	Antiproliferative activity against human A549 cells assessed as inhibition of cell proliferation after 72 hrs by CCK8 assay Antiproliferative activity against human A549 cells assessed as inhibition of cell proliferation after 72 hrs by CCK8 assay	[PMID: 23451797]
HepG2	IC₅₀	1.3 μg/mL Compound: 6	Antiproliferative activity against human HepG2 cells assessed as inhibition of cell proliferation after 72 hrs by CCK8 assay Antiproliferative activity against human HepG2 cells assessed as inhibition of cell proliferation after 72 hrs by CCK8 assay	[PMID: 23451797]
HT-1080	IC₅₀	0.696 μg/mL Compound: 6	Antiproliferative activity against human HT1080 cells assessed as inhibition of cell proliferation after 72 hrs by CCK8 assay Antiproliferative activity against human HT1080 cells assessed as inhibition of cell proliferation after 72 hrs by CCK8 assay	[PMID: 23451797]
HUVEC	IC₅₀	2.4 μg/mL Compound: 6	Antiproliferative activity against human HUVEC assessed as inhibition of cell proliferation after 72 hrs by CCK8 assay Antiproliferative activity against human HUVEC assessed as inhibition of cell proliferation after 72 hrs by CCK8 assay	[PMID: 23451797]
K562	IC₅₀	0.7 μM Compound: 1	In vitro inhibitory activity against K562 human leukemia cell line In vitro inhibitory activity against K562 human leukemia cell line	[PMID: 11212128]
K562	IC₅₀	0.7 μM Compound: 20	Antiproliferative activity against human K562 cells Antiproliferative activity against human K562 cells	10.1039/C2MD20172K
MDA-MB-231	IC₅₀	15 μM Compound: 23	Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay	[PMID: 33650861]
RAW264.7	IC₅₀	1.17 μM Compound: 29	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins before LPS challenge measured 24 hrs after LPS challenge by Griess reaction method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins before LPS challenge measured 24 hrs after LPS challenge by Griess reaction method	[PMID: 21924800]

In Vitro

Alantolactone induces apoptosis in HepG2 cells in a dose-dependent manner. This Alantolactone-induced apoptosis is found to be associated with GSH depletion, inhibition of STAT3 activation, ROS generation, mitochondrial transmembrane potential dissipation, and increased Bax/Bcl-2 ratio and caspase-3 activation^[1]. Alantolactone decreases STAT3 translocation to the nucleus, its DNA-binding, and STAT3 target gene expression. Alantolactone significantly inhibits STAT3 activation with a marginal effect on MAPKs and on NF-κB transcription; however, this effect is not mediated by inhibiting STAT3 upstream kinases^[2].
? Alantolactone induces activin/SMAD3 signaling in human colon adenocarcinoma HCT-8 cells. Alantolactone performs its antitumor effect by interrupting the interaction between Cripto-1 and the activin receptor type IIA in the activin signaling pathway^[4].
? Alantolactone (5 μg/mL, 24 h) inhibits cell proliferation in colon adenocarcinoma HCT-8 cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:

HCT-8 cells.
Concentration:

5 μg/mL (~21.6 μM).
Incubation Time:

24 h.
Result:

Activated the activin signaling pathway in HCT-8 cells.

In Vivo

It is found that the average tumor volume in the Alantolactone-treated mice is approximately 2.17-fold lower compared with that in the control mice. However the administration of Alantolactone does not affect the overall bodyweight during the experimental period, suggesting no apparent toxicity. Additionally, the average tumor weight is significantly lower in the Alantolactone-treated mice compared with the control mice. What’s more, the administration of Alantolactone results in a significant decrease in p-STAT3 and cyclin D1 expression in the tumor tissues^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female athymic BALB/c nude mice at the age of 6 weeks^[2].
Dosage:

2.5 mg/kg.
Administration:

I.P. injection every 2 days.
Result:

Exhibited anti-cancer activity.

Chemical Information

CAS No. 546-43-0
Appearance Solid
Masse moléculaire 232.32
Formule C₁₅H₂₀O₂
Color White to off-white
SMILES

O=C(O[C@@]1([H])[C@]2([H])C=C3[C@@H](C)CCC[C@]3(C)C1)C2=C
Synonyms

(+)-Alantolactone; Alant camphor; Inula camphor
Structure Classification
- Terpenoids
- Sesquiterpenes
Initial Source
- Plants
- other families
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (10)

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Journal Impact Factor

EBioMedicine

Inhibition of HIPK2 protects stress-induced pathological cardiac remodeling. [Abstract]

2022 Nov:85:104274. PMID: 36182775
Pharmacol Res

Cardamonin retards progression of autosomal dominant polycystic kidney disease via inhibiting renal cyst growth and interstitial fibrosis. [Abstract]

2020 May;155:104751. PMID: 32151678
Phytomedicine

Helenine blocks NLRP3 activation by disrupting the NEK7-NLRP3 interaction and ameliorates inflammatory diseases. [Abstract]

2024 Jan:122:155159. PMID: 37931457
ACS Appl Mater Interfaces

Synchronous Sterilization and Macrophage Regulation for Treating Multidrug-Resistant Pseudomonas aeruginosa-Infected Pneumonia. [Abstract]

2025 Oct 8;17(40):55848-55860. PMID: 40987591
Cancer Cell Int

Alantolactone inhibits cell autophagy and promotes apoptosis via AP2M1 in acute lymphoblastic leukemia. [Abstract]

2020 Sep 9;20:442. PMID: 32943990
Front Pharmacol

Selective STAT3 Inhibitor Alantolactone Ameliorates Osteoarthritis via Regulating Chondrocyte Autophagy and Cartilage Homeostasis. [Abstract]

2021 Sep 28:12:730312. PMID: 34650433
Stem Cells

Glycoprotein M6B Interacts with TβRI to Activate TGF-β-Smad2/3 Signaling and Promote Smooth Muscle Cell Differentiation. [Abstract]

2019 Feb;37(2):190-201. PMID: 30372567

Alantolactone purchased from MedChemExpress. Usage Cited in: Stem Cells. 2019 Feb;37(2):190-201. [Abstract]

Western blot images show Smad2/3 phosphorylation is lower than baseline in the sh-GPM6B cells and higher than baseline in the GPM6B-sgRNA cells. The phosphorylation of Smad2/3 in the sh-GPM6B TGF-β1 group is elevated by Alantolactone and the phosphorylation is reduced by treatment with SB431542.

Alantolactone purchased from MedChemExpress. Usage Cited in: Stem Cells. 2019 Feb;37(2):190-201. [Abstract]

Western blot analyses show the repression of SMC specific markers in the sh-GPM6B TGF-β1 group are abolished by Alantolactone in the sh-GPM6B and the enrichment of SMC specific markers in the GPM6B-sgRNA cells is cancelled by treatment with SB431542.
Mamm Genome

Alantolactone curbs the malignant progression of bladder cancer partly via the HSP90AB1/LRP5/β-catenin axis. [Abstract]

2025 Dec 23;37(1):20. PMID: 41436627
Biol Pharm Bull

Alantolactone Induced Apoptosis and DNA Damage of Cervical Cancer through ATM/CHK2 Signaling Pathway. [Abstract]

2024;47(7):1255-1264. PMID: 38972750
bioRxiv

AI-assisted Drug Re-purposing for Human Liver Fibrosis. [Abstract]

2025 May 4:2025.04.29.651320. PMID: 40654780

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (430.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.3044 mL

21.5220 mL

43.0441 mL

5 mM

0.8609 mL

4.3044 mL

8.6088 mL

10 mM

0.4304 mL

2.1522 mL

4.3044 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (10.76 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (10.76 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (10.76 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Khan M, et al. Alantolactone induces apoptosis in HepG2 cells through GSH depletion, inhibition of STAT3 activation, and mitochondrial dysfunction. Biomed Res Int. 2013;2013:719858. [Content Brief]

[2]. Chun J, et al. Alantolactone selectively suppresses STAT3 activation and exhibits potent anticancer activity in MDA-MB-231 cells. Cancer Lett. 2015 Feb 1;357(1):393-403. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.3044 mL	21.5220 mL	43.0441 mL	107.6102 mL
	5 mM	0.8609 mL	4.3044 mL	8.6088 mL	21.5220 mL
	10 mM	0.4304 mL	2.1522 mL	4.3044 mL	10.7610 mL
	15 mM	0.2870 mL	1.4348 mL	2.8696 mL	7.1740 mL
	20 mM	0.2152 mL	1.0761 mL	2.1522 mL	5.3805 mL
	25 mM	0.1722 mL	0.8609 mL	1.7218 mL	4.3044 mL
	30 mM	0.1435 mL	0.7174 mL	1.4348 mL	3.5870 mL
	40 mM	0.1076 mL	0.5381 mL	1.0761 mL	2.6903 mL
	50 mM	0.0861 mL	0.4304 mL	0.8609 mL	2.1522 mL
	60 mM	0.0717 mL	0.3587 mL	0.7174 mL	1.7935 mL
	80 mM	0.0538 mL	0.2690 mL	0.5381 mL	1.3451 mL
	100 mM	0.0430 mL	0.2152 mL	0.4304 mL	1.0761 mL

Keywords

Alantolactone

10 Cited Publications

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Alantolactone

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Publications Citing Use of MedChemExpress (MCE) Alantolactone

Voir tous les produits spécifiques à Isoform STAT

Voir tous les produits spécifiques à Isoform TGF-beta/Smad

Activité biologique

Chemical Information

Publications (10)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Alantolactone Related Classifications

HY-N0038 Related Classifications