Cat. No.: HY-14566

Donepezil

(Synonyms: E2020 free base)

24 Cited Publications

Avis client

Based on 24 publication(s) in Google Scholar

Donepezil (E2020 free base) is a specific and potent AChE inhibitor with IC₅₀s of 8.12 nM and 11.6 nM for bovine AChE and human AChE, respectively.

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

Pureté: 99.82%
CAS No.: 120014-06-4
Formule: C₂₄H₂₉NO₃
Masse moléculaire:379.49

Stockage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Donepezil Chemical Structure

CAS No.: 120014-06-4

Size

Prix

Stock

Quantité

Amount: USD 0.00

* Veuillez sélectionner la quantité avant d'ajouter des articles.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Essai clinique
Chemical Information
Publications (24)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Donepezil (E2020 free base) is a specific and potent AChE inhibitor with IC₅₀s of 8.12 nM and 11.6 nM for bovine AChE and human AChE, respectively^[1].

IC₅₀ & Target

AChE

Voir tous les produits spécifiques à Isoform Cholinesterase (ChE) :

AChE
BChE

Cellular Effect

Cell Line	Type	Value	Description	References
BV-2	IC₅₀	1.54 μM Compound: Donepezil	Cytotoxicity against mouse BV-2 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against mouse BV-2 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 34923430]
CHO	IC₅₀	34.3 μM Compound: donepezil	Inhibition of Cav1.2 current measured using QPatch automatic path clamp system in CHO cells expressing Cav1.2, beta-2 and alpha-2/delta-1 subunits Inhibition of Cav1.2 current measured using QPatch automatic path clamp system in CHO cells expressing Cav1.2, beta-2 and alpha-2/delta-1 subunits	[PMID: 23812503]
HEK293	IC₅₀	>200 μM Compound: Donepezil	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32388114]
HEK293	IC₅₀	0.009 μM Compound: 1	Inhibition of human recombinant AChE expressed in HEK293 cells using acetylthiocholine iodide as substrate preincubated for 30 mins followed by substrate addition measured after 30 mins by Ellman's method Inhibition of human recombinant AChE expressed in HEK293 cells using acetylthiocholine iodide as substrate preincubated for 30 mins followed by substrate addition measured after 30 mins by Ellman's method	[PMID: 26471320]
HEK293	IC₅₀	0.01 μM Compound: Donepezil	Inhibition of recombinant human AChE expressed in HEK293 cells using acetylthiocholine iodide as substrate pretreated for 5 mins followed by substrate addition measured for 5 mins by UV-Vis spectrophotometric method Inhibition of recombinant human AChE expressed in HEK293 cells using acetylthiocholine iodide as substrate pretreated for 5 mins followed by substrate addition measured for 5 mins by UV-Vis spectrophotometric method	[PMID: 28242552]
HEK293	IC₅₀	0.016 μM Compound: donepezil	Inhibition of recombinant human AChE expressed in HEK293 cells preincubated for 15 mins Inhibition of recombinant human AChE expressed in HEK293 cells preincubated for 15 mins	[PMID: 25418133]
HEK293	IC₅₀	6.2 nM Compound: Donepezil	Inhibition of human recombinant AChE expressed in HEK293 cells using acetylthiocholine iodide as substrate by Ellman's method Inhibition of human recombinant AChE expressed in HEK293 cells using acetylthiocholine iodide as substrate by Ellman's method	[PMID: 31304000]
HEK293	IC₅₀	7.5 μM Compound: 1	Inhibition of human recombinant BuChE expressed in HEK293 cells using S-butyrylthiocholine iodide as substrate preincubated for 30 mins followed by substrate addition measured after 30 mins by Ellman's method Inhibition of human recombinant BuChE expressed in HEK293 cells using S-butyrylthiocholine iodide as substrate preincubated for 30 mins followed by substrate addition measured after 30 mins by Ellman's method	[PMID: 26471320]
HEK293	IC₅₀	8.2 μM Compound: donepezil	Inhibition of recombinant human BuChE expressed in HEK293 cells preincubated for 15 mins Inhibition of recombinant human BuChE expressed in HEK293 cells preincubated for 15 mins	[PMID: 25418133]
HepG2	IC₅₀	262727 nM Compound: Donepezil	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 34530383]
HFF	IC₅₀	0.091 μM Compound: Donepezil	Cytotoxicity against HFF cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against HFF cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 32088494]
HT-22	IC₅₀	56.42 μM Compound: Donepezil	Cytotoxicity against mouse HT-22 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against mouse HT-22 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 34923430]
MCF7	IC₅₀	0.06 μM Compound: Donepezil	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 32088494]
NIH3T3	IC₅₀	1.26 μM Compound: Donepezil	Cytotoxicity against mouse NIH/3T3 cells incubated for 24 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells incubated for 24 hrs by MTT assay	[PMID: 38614062]
NIH3T3	IC₅₀	316.42 μM Compound: Donepezil	Cytotoxicity against mouse NIH/3T3 cells after 24 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells after 24 hrs by MTT assay	[PMID: 27789142]
Sf21	IC₅₀	205.7 μM Compound: Donepezil	Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake	[PMID: 21965623]
Sf21	IC₅₀	519.3 μM Compound: Donepezil	Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake	[PMID: 21965623]
SH-SY5Y	CC₅₀	25.06 μM Compound: Donepezil	Cytotoxicity in human SH-SY5Y cells assessed as reduction in cell viability after 72 hrs incubation by MTT assay Cytotoxicity in human SH-SY5Y cells assessed as reduction in cell viability after 72 hrs incubation by MTT assay	[PMID: 30822710]
SH-SY5Y	IC₅₀	>200 μM Compound: Donepezil	Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32388114]
SH-SY5Y	IC₅₀	0.3 μM Compound: Donepezil	Cytotoxicity against human SH-SY5Y cells measured after 48 hrs by MTT assay Cytotoxicity against human SH-SY5Y cells measured after 48 hrs by MTT assay	[PMID: 32088494]
SH-SY5Y	IC₅₀	15.54 μM Compound: 1	Cytotoxicity against human SH-SY5Y cells after 72 hrs by SRB assay Cytotoxicity against human SH-SY5Y cells after 72 hrs by SRB assay	[PMID: 27823887]
SH-SY5Y	IC₅₀	155 μM Compound: 2; Don	Antialzheimer activity in human SH-SY5Y cells assessed as inhibition of GA-induced tau hyperphosphorylation at Ser396 residue incubated for 24 hrs by ELISA Antialzheimer activity in human SH-SY5Y cells assessed as inhibition of GA-induced tau hyperphosphorylation at Ser396 residue incubated for 24 hrs by ELISA	[PMID: 36257284]
SH-SY5Y	IC₅₀	202 μM Compound: 2; Don	Antioxidant activity against okadaic acid-induced ROS generation in human SH-SY5Y cells assessed as reduction in DCF fluorescence intensity pretreated for 24 hrs followed by okadaic acid addition for 24 hrs by DCFH-DA dye based fluorescence analysis Antioxidant activity against okadaic acid-induced ROS generation in human SH-SY5Y cells assessed as reduction in DCF fluorescence intensity pretreated for 24 hrs followed by okadaic acid addition for 24 hrs by DCFH-DA dye based fluorescence analysis	[PMID: 36257284]
SH-SY5Y	IC₅₀	326 μM Compound: 2; Don	Neuroprotective activity in human SH-SY5Y cells assessed as inhibition of okadaic acid-induced mitochondrial membrane potential by measuring increase in red-to-green fluorescence intensity ratio pretreated for 24 hrs followed by okadaic acid addition for Neuroprotective activity in human SH-SY5Y cells assessed as inhibition of okadaic acid-induced mitochondrial membrane potential by measuring increase in red-to-green fluorescence intensity ratio pretreated for 24 hrs followed by okadaic acid addition for	[PMID: 36257284]
SH-SY5Y	IC₅₀	35 μM Compound: 2; Don	Antialzheimer activity in human SH-SY5Y cells assessed as inhibition of GA-induced tau hyperphosphorylation at Ser199 residue incubated for 24 hrs by ELISA Antialzheimer activity in human SH-SY5Y cells assessed as inhibition of GA-induced tau hyperphosphorylation at Ser199 residue incubated for 24 hrs by ELISA	[PMID: 36257284]
SH-SY5Y	IC₅₀	84.1 μM Compound: Donepezil	Neurotoxicity against human SH-SY5Y cells assessed as inhibition of cell viability incubated for 24 hrs by MTT assay Neurotoxicity against human SH-SY5Y cells assessed as inhibition of cell viability incubated for 24 hrs by MTT assay	[PMID: 30795991]
SH-SY5Y	IC₅₀	95.13 μM Compound: 1	Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay	[PMID: 31280020]
SK-N-MC	IC₅₀	>100 μM Compound: Donepezil	Antiproliferative activity against human SK-N-MC cells after 72 hrs by MTT assay Antiproliferative activity against human SK-N-MC cells after 72 hrs by MTT assay	[PMID: 28841514]
U-87MG ATCC	IC₅₀	47.24 μM Compound: V	Cytotoxicity against human U-87 MG cells assessed as inhibition of cell growth by MTT assay Cytotoxicity against human U-87 MG cells assessed as inhibition of cell growth by MTT assay	[PMID: 36630883]

In Vitro

Donepezil (E2020 free base) inhibits the carbachol-stimulated increase in intracellular Ca2+ concentration in human SHSY5Y neuroblastoma cells in a concentration dependent manner, indicating that Donepezil have muscarinic antagonist activity. Intraperitoneal administration of Donepezil in rats produces a dose dependent increase in salivation and tremor, which are overt cholinergic behavioural signs, with an ED50 of 6 μmol/kg. Donepezil is found to be somewhat less potent with a ED50 of 50 μmol/kg following oral administration^[2].
A recent study shows that Donepezil can protect human umbilical vein endothelial cells (HUVECs) against H2O2-induced cell injury. This may be useful as a potential therapy for oxidative stress in cardiovascular and cerebrovascular diseases^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Essai clinique

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 120014-06-4
Appearance Solid
Masse moléculaire 379.49
Formule C₂₄H₂₉NO₃
Color White to off-white
SMILES

O=C(C(C=C(OC)C(OC)=C1)=C1C2)C2CC(CC3)CCN3CC4=CC=CC=C4
Synonyms

E2020 free base
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (24)

All In Vivo Efficacy StudyIFWBHistological Imaging/Staining

Sort by:

Journal Impact Factor

Cancer Cell

Long-range cholinergic input promotes glioblastoma progression. [Abstract]

2025 Nov 10;43(11):2089-2105.e10. PMID: 40829591
Phytomedicine

Modified Kai-Xin-San ameliorates cognitive impairment in Alzheimer's disease model mice by regulating neuroinflammation and synaptic dysfunction. [Abstract]

2026 Jun:155:158102. PMID: 41931996
Phytomedicine

Pterostilbene mitigates scopolamine-induced cognitive impairment by modulating cholinergic pathway and reducing neuroinflammation. [Abstract]

2026 Jan:150:157630. PMID: 41353883
Phytomedicine

Zexieyin formula alleviates Alzheimer's disease via post-synaptic CaMKII modulating AMPA receptor: Involved in promoting neurogenesis to strengthen synaptic plasticity in mice hippocampus. [Abstract]

2024 Aug:131:155802. PMID: 38852473

Donepezil purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Aug:131:155802. [Abstract]

Donepezil (Don: 5 mg/kg). The latency of the mice swimming to the platform in MWM test.

Donepezil purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Aug:131:155802. [Abstract]

Donepezil (Don: 5 mg/kg). Immunofluorescence staining of Brdu (red) and DCX (green) with DAPI (blue) in hippocampus.

Donepezil purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Aug:131:155802. [Abstract]

Donepezil (Don: 5 mg/kg). Representative Western blot images.
Alzheimers Res Ther

Pathway-based network medicine identifies novel natural products for Alzheimer's disease. [Abstract]

2025 Feb 14;17(1):43. PMID: 39953559

Donepezil purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Feb 14;17(1):43. [Abstract]

APP/PS1 mice were administered Donepezil Hydrochloride intraperitoneally at 1 mg/kg daily for three months to perform the Y-maze test.

Donepezil purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Feb 14;17(1):43. [Abstract]

APP/PS1 mice were administered Donepezil Hydrochloride intraperitoneally at 1 mg/kg daily for three months to test plateau quadrant dwell time.

Donepezil purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Feb 14;17(1):43. [Abstract]

APP/PS1 mice were administered Donepezil Hydrochloride intraperitoneally at 1 mg/kg daily for three months to test their plateau quadrant swimming distance.

Donepezil purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Feb 14;17(1):43. [Abstract]

APP/PS1 mice were intraperitoneally injected daily with Donepezil Hydrochloride at 1 mg/kg for 6E10 staining of the cortex and hippocampus for up to three months.
Clin Transl Med

Medial septum tau accumulation induces spatial memory deficit via disrupting medial septum-hippocampus cholinergic pathway. [Abstract]

2021 Jun;11(6):e428. PMID: 34185417

Donepezil purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2021 Jun;11(6):e428. [Abstract]

Donepezil Hydrochloride at 1.0 or 2.0 mg/kg/day was injected intraperitoneally at 5 months after MS AAV‐CAG‐hTau‐mCherry‐3flag or AAV‐CAG‐vector‐mCherry‐3flag infusion. Both doses of Donepezil Hydrochloride rescued synaptic transmission in MShTau mice, as evidenced by increased input-output responses.

Donepezil purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2021 Jun;11(6):e428. [Abstract]

Five months after infusion of MS AAV-CAG-hTau-mCherry-3flag or AAV-CAG-vector-mCherry-3flag, Donepezil Hydrochloride was administered intraperitoneally at doses of 1.0 or 2.0 mg/kg/day. Both doses of Donepezil Hydrochloride rescued synaptic transmission in MShTau mice, manifested as an increased fEPSP slope induced during LTP induction, and this increase remained significant 60 minutes after high-frequency stimulation (HFS).
J Ethnopharmacol

Lancao decoction alleviates Alzheimer's disease: Depending on activating CaMKII to protect neuronal refunction by reducing β-amyloid in the hippocampus. [Abstract]

2025 Apr 9:345:119619. PMID: 40074096
J Ethnopharmacol

Lancao decoction in the treatment of alzheimer's disease via activating PI3K/AKT signaling to promote ERK involving in enhancing neuronal activities in the hippocampus. [Abstract]

2024 Nov 9;338(Pt 1):119017. PMID: 39528121
Eur J Med Chem

Design, synthesis and biological evaluation of carbamate derivatives incorporating multifunctional carrier scaffolds as pseudo-irreversible cholinesterase inhibitors for the treatment of Alzheimer's disease. [Abstract]

2024 Feb 5:265:116071. PMID: 38157596

Donepezil purchased from MedChemExpress. Usage Cited in: Eur J Med Chem. 2024 Feb 5:265:116071. [Abstract]

Effects of C7 and Donepezil (DZ, 5 mg/kg) on AChE activity in mouse brain.

Donepezil purchased from MedChemExpress. Usage Cited in: Eur J Med Chem. 2024 Feb 5:265:116071. [Abstract]

Effects of C7 and Donepezil (DZ, 5 mg/kg) on Aβ1-42-induced learning impairment in mice: spontaneous alternation behavior.
Food Biosci

2025 Sep.
Foods

Sea Cucumber Hydrolysates Alleviate Cognitive Deficits in D-Galactose-Induced C57BL/6J Aging Mice Associated with Modulation of Gut Microbiota. [Abstract]

2025 May 29;14(11):1938. PMID: 40509464
Foods

Improving Effect of the Policosanol from Ericerus pela Wax on Learning and Memory Impairment Caused by Scopolamine in Mice. [Abstract]

2022 Jul 14;11(14):2095. PMID: 35885338
Int J Mol Sci

Structural Bioinformatics Applied to Acetylcholinesterase Enzyme Inhibition. [Abstract]

2025 Apr 17;26(8):3781. PMID: 40332446
Int Immunopharmacol

Farrerol alleviates cognitive impairments in chronic cerebral hypoperfusion via suppressing NLRP3 inflammasome-mediated pyroptosis. [Abstract]

2025 Apr 24:153:114442. PMID: 40112599
Comput Struct Biotechnol J

Network Proximity-based computational pipeline identifies drug candidates for different pathological stages of Alzheimer's disease. [Abstract]

2023 Feb 24:21:1907-1920. PMID: 36936813
Neuropharmacology

Ajugol ameliorates mitochondrial dysfunction and cognitive decline in Alzheimer's Disease via BNIP3-dependent mitophagy. [Abstract]

2026 Jun 15:291:110923. PMID: 41819485
Journal of Pharmaceutical Investigation

2021 Mar 31.
Mol Brain

Target oxidative stress-induced disulfidptosis: novel therapeutic avenues in Parkinson's disease. [Abstract]

2025 Apr 4;18(1):29. PMID: 40186271
J Integr Neurosci

Cognitive Enhancer Donepezil Attenuates Heroin-Seeking Behavior Induced by Cues in Rats. [Abstract]

2023 May 16;22(3):76. PMID: 37258429
Chem Biodivers

Protocatechuic Acid Improves Alzheimer's Disease by Regulating the Cholinergic Synaptic Signaling Pathway. [Abstract]

2025 May;22(5):e202402771. PMID: 39776239
Neurol Res

Polygonatum sibiricum polysaccharide ameliorates neurotoxicity in Alzheimer disease mice by inhibiting endoplasmic reticulum stress and lipid raft formation through AMPK/GSK3β/Nrf2 pathway. [Abstract]

2025 Sep 13:1-18. PMID: 40946159
bioRxiv

In vivo multiplex imaging of dynamic neurochemical networks with designed far-red dopamine sensors. [Abstract]

2024 Dec 23:2024.12.22.629999. PMID: 39763912
Research Square Preprint

2024 Apr 23.
Patent

US20210378961A1.

Solvant et solubilité

In Vitro:

DMSO : 33.33 mg/mL (87.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6351 mL

13.1756 mL

26.3512 mL

5 mM

0.5270 mL

2.6351 mL

5.2702 mL

10 mM

0.2635 mL

1.3176 mL

2.6351 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (274 KB)
SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Ogura, H., et al., Comparison of inhibitory activities of donepezil and other cholinesterase inhibitors on acetylcholinesterase and butyrylcholinesterase in vitro. Methods Find Exp Clin Pharmacol, 2000. 22(8): p. 609-13. [Content Brief]

[2]. Snape, M.F., et al., A comparative study in rats of the in vitro and in vivo pharmacology of the acetylcholinesterase inhibitors tacrine, donepezil and NXX-066. Neuropharmacology, 1999. 38(1): p. 181-93. [Content Brief]

[3]. Huang, Z.H., et al., Donepezil protects endothelial cells against hydrogen peroxide-induced cell injury. CNS Neurosci Ther, 2012. 18(2): p. 185-7. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6351 mL	13.1756 mL	26.3512 mL	65.8779 mL
	5 mM	0.5270 mL	2.6351 mL	5.2702 mL	13.1756 mL
	10 mM	0.2635 mL	1.3176 mL	2.6351 mL	6.5878 mL
	15 mM	0.1757 mL	0.8784 mL	1.7567 mL	4.3919 mL
	20 mM	0.1318 mL	0.6588 mL	1.3176 mL	3.2939 mL
	25 mM	0.1054 mL	0.5270 mL	1.0540 mL	2.6351 mL
	30 mM	0.0878 mL	0.4392 mL	0.8784 mL	2.1959 mL
	40 mM	0.0659 mL	0.3294 mL	0.6588 mL	1.6469 mL
	50 mM	0.0527 mL	0.2635 mL	0.5270 mL	1.3176 mL
	60 mM	0.0439 mL	0.2196 mL	0.4392 mL	1.0980 mL
	80 mM	0.0329 mL	0.1647 mL	0.3294 mL	0.8235 mL

Keywords

Donepezil

24 Cited Publications

100 mg Obtenir un devis

Subscribe to the

MedChemExpress

Newsletter

Nom du produit:
Cat. No.:
Quantité:
MCE Japan Authorized Agent:

Nom du produit:
CAS No.:
Species:
Pureté:
Quantité:

Donepezil

Galanthamine

Rivastigmine

Donepezil Hydrochloride

Pyridostigmine bromide

Neostigmine Bromide

DTNB

SB-431542

Irinotecan

Semaglutide

D-Galactose

Ilomastat

Lithium chloride, 99.3%-T

Publications Citing Use of MedChemExpress (MCE) Donepezil

Voir tous les produits spécifiques à Isoform Cholinesterase (ChE)

Activité biologique

Chemical Information

Publications (24)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Donepezil

Galanthamine

Rivastigmine

Donepezil Hydrochloride

Pyridostigmine bromide

Neostigmine Bromide

DTNB

SB-431542

Irinotecan

Semaglutide

D-Galactose

Ilomastat

Lithium chloride, 99.3%-T

Publications Citing Use of MedChemExpress (MCE) Donepezil

Voir tous les produits spécifiques à Isoform Cholinesterase (ChE)

Activité biologique

Chemical Information

Publications (24)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Donepezil Related Classifications

HY-14566 Related Classifications