Sulforaphene
Based on 2 publication(s) in Google Scholar
Sulforaphene, isolated from radish seeds, exhibits an ED50 against velvetleaf seedlings approximately 2 x 10-4 M. Sulforaphene promotes cancer cells apoptosis and inhibits migration via inhibiting EGFR, p-ERK1/2, NF‐κB and other signals.
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- Pureté : 99.78%
- CAS No.: 592-95-0
- Formule: C6H9NOS2
- Masse moléculaire:175.27
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Stockage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Sulforaphene
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Cell Proliferation/Viability Assay
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Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| A-375 | IC50 |
18.74 μM
Compound: SFE
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Antiproliferative activity against human A375 cells after 72 hrs by CCK8 assay
Antiproliferative activity against human A375 cells after 72 hrs by CCK8 assay
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[PMID: 30282324] |
| A549 | IC50 |
9.33 μM
Compound: SFE
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Antiproliferative activity against human A549 cells after 72 hrs by CCK8 assay
Antiproliferative activity against human A549 cells after 72 hrs by CCK8 assay
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[PMID: 30282324] |
| HCT-116 | IC50 |
31.92 μM
Compound: SFE
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Antiproliferative activity against human HCT116 cells after 72 hrs by CCK8 assay
Antiproliferative activity against human HCT116 cells after 72 hrs by CCK8 assay
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[PMID: 30282324] |
| HeLa | IC50 |
85.05 μM
Compound: SFE
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Antiproliferative activity against human HeLa cells after 72 hrs by CCK8 assay
Antiproliferative activity against human HeLa cells after 72 hrs by CCK8 assay
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[PMID: 30282324] |
| SMMC-7721 | IC50 |
6.41 μM
Compound: SFE
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Antiproliferative activity against human SMMC7721 cells after 72 hrs by CCK8 assay
Antiproliferative activity against human SMMC7721 cells after 72 hrs by CCK8 assay
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[PMID: 30282324] |
In Vitro
Sulforaphene (0-80 μM, 48 h) inhibits HCC (HepG2 and Hep3B cells) cell viability and induces apoptosis[4].
Sulforaphene (0-40 μM) sensitizes radiosensitivity of HepG2 and Hep3B cells, and promotes radiation-induced cell death[4].
Sulforaphene (0-10 μM, 24 h) inhibits SUM159 cell migration and invasion by inhibiting Hedgehog signaling[5].
Sulforaphene (5 μM, 24 h) inhibits microtubule polymerization in HCT116 and HT-29 cells[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HepG2 and Hep3B cell
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Concentration:0-40 μM
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Incubation Time:48 h
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Result:Increased Bax expression and reduced Bcl-2 expression.
Inhibited expression of NF-𝜅B-dependent genes (COX-2, iNos, and cyclinD1).
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mouse HCT116 xenograft model[6]
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Dosage:1 and 5 mg/kg
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Administration:i.p., everyday
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Result:Inhibited tumor growth, and increased CDK1, MK2, and p38 phosphorylation.
Chemical Information
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CAS No. 592-95-0
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Appearance Liquid (Density: 1.16±0.1 g/cm3)
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Masse moléculaire 175.27
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Formule C6H9NOS2
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Color Colorless to light yellow
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SMILES
O=S(/C=C/CCN=C=S)C
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Cell Rep
Shear stress-induced Piezo1 activates CD99L2 to facilitate the initiation of blood circulation. [Abstract]2026 Mar 30;45(4):117200. PMID: 41915472 -
J Evid Based Integr Med
Radish Seed Exerts Anti-Diabetic and Obesity-Reducing Effects in Mice by Promoting the Activation of Uncoupling Protein 1 and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α. [Abstract]2025 Jan-Dec:30:2515690X251316760. PMID: 39905916
Sulforaphene purchased from MedChemExpress. Usage Cited in: J Evid Based Integr Med. 2025 Jan-Dec:30:2515690X251316760. [Abstract]
Sulforaphene (SE) (0, 200, 100, 50, 25, 20, 10, 5, and 1 μM) and cell viability was determined.
Sulforaphene purchased from MedChemExpress. Usage Cited in: J Evid Based Integr Med. 2025 Jan-Dec:30:2515690X251316760. [Abstract]
Cells were treated with NC, PC and Sulforaphene (SE) (20-0.3125 μM) for eight days. Western blot analysis of PGC1α and UCP1.
Sulforaphene purchased from MedChemExpress. Usage Cited in: J Evid Based Integr Med. 2025 Jan-Dec:30:2515690X251316760. [Abstract]
The change of body mass treated with Sulforaphene (SE) (5, 50 mg/kg, p.o.).
Sulforaphene purchased from MedChemExpress. Usage Cited in: J Evid Based Integr Med. 2025 Jan-Dec:30:2515690X251316760. [Abstract]
Representative sections of liver with H&E staining treated with Sulforaphene (SE) (5, 50 mg/kg, p.o.).
Sulforaphene purchased from MedChemExpress. Usage Cited in: J Evid Based Integr Med. 2025 Jan-Dec:30:2515690X251316760. [Abstract]
Immunofluorescence staining for UCP1, ATP5A, and DAPI (blue) in SAT treated with Sulforaphene (SE) (5, 50 mg/kg, p.o.).
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (570.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.26 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.26 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocole
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Detection of Apoptosis
Apoptosis, also called programmed cell death, is generally characterized by distinct morphological characteristics.
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TUNEL staining for apoptotic DNA fragmentation
TUNEL staining detects DNA strand breaks by using terminal deoxynucleotidyl transferase to add labeled nucleotides to exposed 3′-OH DNA termini, generating either microscopic staining in fixed cells or tissue sections, or fluorescence/cytometric signal in cell suspensions. TUNEL positivity reflects DNA fragmentation but should not be interpreted alone as definitive apoptosis, because TUNEL can also label necrotic, autolytic, mechanically damaged, or DNA-repair-associated DNA breaks.
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Annexin V plus membrane-impermeant dye apoptosis staining
Annexin V-based apoptosis assays rely on the detection of phosphatidylserine (PS) externalization from the inner leaflet of the plasma membrane to the outer leaflet, an early biochemical hallmark of apoptosis. Fluorescently labeled Annexin V binds PS in a calcium-dependent manner, enabling identification of early apoptotic cells by flow cytometry or fluorescence microscopy. When combined with a membrane-impermeant DNA-binding dye (e. g. , propidium iodide), this approach allows discrimination between viable (Annexin V−/dye−), early apoptotic (Annexin V+/dye−), and late apoptotic or necrotic (Annexin V+/dye+) cell populations by assessing membrane integrity and PS exposure.
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Cell migration
Cell migration is a method that plays an important role in wound healing, cell differentiation, embryonic development, etc.
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Apoptosis Solutions
Apoptosis is a regulated, generally non-lytic cell-death pathway that removes unwanted, damaged, infected, or abnormal cells through coordinated morphological changes, caspase activation, DNA fragmentation, and membrane remodeling. The intrinsic apoptosis pathway is controlled mainly by mitochondrial outer membrane permeabilization, BCL-2 family proteins, cytochrome c release, apoptosome formation, caspase-9 activation, and downstream executioner caspase-3/7 activation. The extrinsic apoptosis pathway is initiated by death receptors such as Fas, TNFR, and TRAIL receptors, which recruit adaptor proteins and activate caspase-8 before engaging executioner caspases or mitochondrial amplification through BID cleavage. Apoptosis is linked to many phenotypes, including cancer cell killing, tissue homeostasis, immune regulation, neurodegeneration, infection response, and treatment-induced cytotoxicity; unresolved questions include how apoptosis interacts with necroptosis, pyroptosis, ferroptos
Pureté et documentation
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Fiche technique (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
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- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Kuang P, et al. Separation and purification of sulforaphene from radish seeds using macroporous resin and preparative high-performance liquid chromatography. Food Chem. 2013 Jan 15;136(2):342-7. [Content Brief]
[4]. Ren K, et al. Sulforaphene enhances radiosensitivity of hepatocellular carcinoma through suppression of the NF-κB pathway. J Biochem Mol Toxicol. 2017 Aug;31(8). [Content Brief]
[5]. Bao C, et al. Sulforaphene Interferes with Human Breast Cancer Cell Migration and Invasion through Inhibition of Hedgehog Signaling. J Agric Food Chem. 2016 Jul 13;64(27):5515-24. [Content Brief]
[6]. Byun S, et al. Sulforaphene suppresses growth of colon cancer-derived tumors via induction of glutathione depletion and microtubule depolymerization. Mol Nutr Food Res. 2016 May;60(5):1068-78. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.7055 mL | 28.5274 mL | 57.0548 mL | 142.6371 mL |
| 5 mM | 1.1411 mL | 5.7055 mL | 11.4110 mL | 28.5274 mL | |
| 10 mM | 0.5705 mL | 2.8527 mL | 5.7055 mL | 14.2637 mL | |
| 15 mM | 0.3804 mL | 1.9018 mL | 3.8037 mL | 9.5091 mL | |
| 20 mM | 0.2853 mL | 1.4264 mL | 2.8527 mL | 7.1319 mL | |
| 25 mM | 0.2282 mL | 1.1411 mL | 2.2822 mL | 5.7055 mL | |
| 30 mM | 0.1902 mL | 0.9509 mL | 1.9018 mL | 4.7546 mL | |
| 40 mM | 0.1426 mL | 0.7132 mL | 1.4264 mL | 3.5659 mL | |
| 50 mM | 0.1141 mL | 0.5705 mL | 1.1411 mL | 2.8527 mL | |
| 60 mM | 0.0951 mL | 0.4755 mL | 0.9509 mL | 2.3773 mL | |
| 80 mM | 0.0713 mL | 0.3566 mL | 0.7132 mL | 1.7830 mL | |
| 100 mM | 0.0571 mL | 0.2853 mL | 0.5705 mL | 1.4264 mL |
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