Amiloride hydrochloride dihydrate
Based on 72 publication(s) in Google Scholar
Amiloride hydrochloride dihydrate (MK-870 hydrochloride dihydrate) is an inhibitor of both epithelial sodium channel (ENaC) and urokinase-type plasminogen activator receptor (uTPA). Amiloride hydrochloride dihydrate is a blocker of polycystin-2 (PC2; TRPP2) channel.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.88%
- CAS. Nr.: 17440-83-4
- Formel: C6H13Cl2N7O3
- Molecular Weight:302.12
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Speicherung:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Publications Citing Use of MedChemExpress (MCE) Amiloride hydrochloride dihydrate
More- Signal Transduct Target Ther. 2026 May 13;11(1):183. [Abstract]
- Nat Nanotechnol. 2024 Dec;19(12):1903-1913. [Abstract]
- Cell Metab. 2022 Dec 6;34(12):2018-2035.e8. [Abstract]
- Adv Mater. 2026 Jun;38(33):e21108. [Abstract]
- Nat Biomed Eng. 2026 Jun 3. [Abstract]
- Nat Biomed Eng. 2024 Nov;8(11):1469-1482. [Abstract]
- Cell Mol Immunol. 2024 Aug;21(8):826-841. [Abstract]
- J Extracell Vesicles. 2026 Feb;15(2):e70238. [Abstract]
- Adv Funct Mater. 2025 Aug 3.
- Adv Funct Mater. 2024 Oct 31.
- Mol Neurodegener. 2025 Jul 23;20(1):84. [Abstract]
- ACS Nano. 2026 Jul 28;20(29):20730-20746.
- ACS Nano. 2026 Jun 26. [Abstract]
- ACS Nano. 2025 Dec 26. [Abstract]
- ACS Nano. 2023 May 9;17(9):8141-8152. [Abstract]
- J Am Chem Soc. 2018 Dec 12;140(49):17234-17240. [Abstract]
- J Nanobiotechnology. 2026 Jul 3.
- J Nanobiotechnology. 2020 Jan 31;18(1):26. [Abstract]
- Theranostics. 2020 May 17;10(15):6581-6598. [Abstract]
- Theranostics. 2019 May 31;9(13):3732-3753. [Abstract]
- Acta Pharm Sin B. 2024 Dec;14(12):5418-5434. [Abstract]
- Adv Sci (Weinh). 2025 Jun;12(21):e2406150. [Abstract]
- Adv Sci (Weinh). 2025 May;12(20):e2412356. [Abstract]
- Adv Sci (Weinh). 2024 Mar;11(9):e2307880. [Abstract]
- Cell Rep Med. 2025 Jul 15;6(7):102208. [Abstract]
- Biomaterials. 2026 Jan:324:123541. [Abstract]
- Biomaterials. 2022 May;284:121529. [Abstract]
- J Control Release. 2026 Jan 10:389:114450. [Abstract]
- J Control Release. 2024 Dec:376:1316-1328. [Abstract]
- Cell Mol Biol Lett. 2024 Dec 3;29(1):149. [Abstract]
- Environ Sci Technol. 2021 Mar 2;55(5):3144-3155. [Abstract]
- Small. 2026 Jun 5:e74067. [Abstract]
- Biosens Bioelectron. 2025 Apr 15:274:117184. [Abstract]
- Cell Commun Signal. 2023 May 4;21(1):99. [Abstract]
- J Neuroinflammation. 2022 Feb 22;19(1):53. [Abstract]
- J Pharm Anal. 2025 Jul;15(7):101182. [Abstract]
- Mater Today Bio. 2025 Mar 27:32:101712. [Abstract]
- J Hazard Mater. 2025 Dec 5:500:140498. [Abstract]
- Acta Biomater. 2025 Jul 1:201:171-186. [Abstract]
- Food Chem. 2025 Jan 15;463(Pt 1):141196. [Abstract]
- Proc Natl Acad Sci U S A. 2026 Mar 31;123(13):e2520119123. [Abstract]
- Food Res Int. 2025 Dec 20;226:118114.
- Int J Biol Macromol. 2024 Aug 5;277(Pt 3):134517. [Abstract]
- Int J Biol Macromol. 2022 May 15:207:559-569. [Abstract]
- Small Methods. 2021 Apr;5(4):e2001047. [Abstract]
- Cell Rep. 2022 May 31;39(9):110880. [Abstract]
- Br J Pharmacol. 2020 Aug;177(15):3473-3488. [Abstract]
- Anal Chem. 2026 Feb 10;98(5):3577-3587. [Abstract]
- Anal Chem. 2025 Jun 17;97(23):12026-12034. [Abstract]
- J Ethnopharmacol. 2022 Jun 28:292:115171. [Abstract]
- J Cell Biol. 2025 May 5;224(5):e202405060. [Abstract]
- Transl Res. 2024 Jan:263:53-72. [Abstract]
- Commun Biol. 2022 Nov 14;5(1):1248. [Abstract]
- J Zhejiang Univ Sci B. 2025 May 28;26(5):461-476. [Abstract]
- mBio. 2025 Mar 12;16(3):e0378724. [Abstract]
- Mol Pharm. 2025 Apr 7;22(4):2182-2192. [Abstract]
- iScience. 2022 Oct 19;25(12):105403. [Abstract]
- FASEB J. 2025 Jan 15;39(1):e70298. [Abstract]
- Microb Pathog. 2019 Oct:135:103638. [Abstract]
- Nanotoxicology. 2026 May 19:1-18. [Abstract]
- J Leukoc Biol. 2024 Sep 2;116(3):565-578. [Abstract]
- J Pharm Biomed Anal. 2021 Feb 20:195:113870. [Abstract]
- Nanotechnology. 2018 Feb 23;29(8):085101. [Abstract]
- Cell Physiol Biochem. 2018;51(6):2564-2574. [Abstract]
- Am J Transl Res. 2021 Jul 15;13(7):7538-7555. [Abstract]
- Res Sq. 2026 Jun 1.
- NPJ Biomed Innov. 2026 May 22;3(1):33. [Abstract]
- Res Sq. 2026 Feb 5.
- Res Sq. 2025 Nov 16.
- SSRN. 2025 Aug 21.
- University of Toronto. 2025.
- Sci Total Environ. 2021 Jul 20:779:146523. [Abstract]
Customer Validation & Images
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In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 17440-83-4
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Appearance Solid
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Molecular Weight 302.12
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Formel C6H13Cl2N7O3
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Color Off-white to yellow
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SMILES
O=C(C1=NC(Cl)=C(N)N=C1N)NC(N)=N.[H]Cl.O.O
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Synonyms
MK-870 hydrochloride dihydrate
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (72)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Disrupting SOX2 self-association and condensate formation to overcome chemotherapeutic drug resistance in lung squamous cell carcinoma. [Abstract]2026 May 13;11(1):183. PMID: 42143069 -
Nat Nanotechnol
Metal-ion-chelating phenylalanine nanostructures reverse immune dysfunction and sensitize breast tumour to immune checkpoint blockade. [Abstract]2024 Dec;19(12):1903-1913. PMID: 39187583 -
Cell Metab
Cancer cell-intrinsic XBP1 drives immunosuppressive reprogramming of intratumoral myeloid cells by promoting cholesterol production. [Abstract]2022 Dec 6;34(12):2018-2035.e8. PMID: 36351432
Amiloride hydrochloride dihydrate purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Dec 6;34(12):2018-2035.e8. [Abstract]
Bone marrow cells cultured in medium containing 10 ng/mL GM-CSF for 3 days were treated with 2 μg PKH67-labeled sEVs with or without Amiloride (10 μM), Chlorpromazine (10 μM), or EIPA (5 μM) for 1 h. The median fluorescence intensity (MFI) of PKH67 was assessed by flow cytometry.
Amiloride hydrochloride dihydrate purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Dec 6;34(12):2018-2035.e8. [Abstract]
Amiloride hydrochloride (10 μM; 3 d) crippled the effect of shNT cell-derived sEVs in elevating MDSCs to the level of shXBP cell-generated sEVs.
Amiloride hydrochloride dihydrate purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Dec 6;34(12):2018-2035.e8. [Abstract]
Amiloride hydrochloride (10 μM; 3 d) decreased the STAT3 phosphorylation level in MDSCs.
Amiloride hydrochloride dihydrate purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Dec 6;34(12):2018-2035.e8. [Abstract]
Amiloride hydrochloride (10 μM; 3 d) decreased the iNOS mRNA level in MDSCs.
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Adv Mater
Conformationally Variable Peptides Trap and Detoxify Ox-LDL in Plaques for Attenuating Atherosclerosis in Multiple Species. [Abstract]2026 Jun;38(33):e21108. PMID: 42116761 -
Nat Biomed Eng
Modular nucleic acid-based construct for delivery of immunostimulatory agonists and oncogene-silencing oligonucleotides in tumours. [Abstract]2026 Jun 3. PMID: 42236980 -
Nat Biomed Eng
In situ formation of biomolecular condensates as intracellular drug reservoirs for augmenting chemotherapy. [Abstract]2024 Nov;8(11):1469-1482. PMID: 39271933 -
Cell Mol Immunol
Depleting profibrotic macrophages using bioactivated in vivo assembly peptides ameliorates kidney fibrosis. [Abstract]2024 Aug;21(8):826-841. PMID: 38871810 -
J Extracell Vesicles
Extracellular Vesicles Coordinate Bacterial Cloaking in Lung Epithelial Cells to Alleviate Acute Inflammatory Injury. [Abstract]2026 Feb;15(2):e70238. PMID: 41656978 -
Amiloride hydrochloride dihydrate purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Aug 3.
Typical confocal images of RAW 264.7 cells incubated with 100 µg/mL FITC/M-BSA@PSiNPs for 6 h, in the absence or presence of Amiloride（50 μM; 6 h).
Amiloride hydrochloride dihydrate purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Aug 3.
The inhibitory effects of Pitstop 2, MβCD, Amiloride (50 μM; 6 h) and CytoD on the expression of IL-1β, IL-6, CCL2, IFN-β, CXCL10 and TNF-α genes in RAW 264.7 cells.
Amiloride hydrochloride dihydrate purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Aug 3.
Typical confocal images of RAW 264.7 cells incubated with 100 μg/mL FITC/M-BSA@PSiNPs for 6 h, in the absence or presence of 50 μmol/L Pitstop 2, 100 μmol/L Methyl-β-cyclodextrin (MβCD), 50 μmol/L amiloride, or 1 μmol/L CytoD (scale bar = 40 μm).
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Mol Neurodegener
Endothelium-specific endoglin triggers astrocyte reactivity via extracellular vesicles in a mouse model of Alzheimer's disease. [Abstract]2025 Jul 23;20(1):84. PMID: 40702549 -
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ACS Nano
Autophagy-Inducing and cfDNA-Scavenging Nanoparticles for Synergistic Atherosclerosis Therapy. [Abstract]2026 Jun 26. PMID: 42361347 -
ACS Nano
Redox-Responsive Peptide Coacervates for Enhanced mRNA Delivery and Intracellular Release. [Abstract]2025 Dec 26. PMID: 41451635 -
ACS Nano
Enantioselective Degrader for Elimination of Extracellular Aggregation-Prone Proteins hIAPP Associated with Type 2 Diabetes. [Abstract]2023 May 9;17(9):8141-8152. PMID: 37057955 -
J Am Chem Soc
2018 Dec 12;140(49):17234-17240. PMID: 30398334 -
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J Nanobiotechnology
Enhance transgene responses through improving cellular uptake and intracellular trafficking by bio-inspired non-viral vectors. [Abstract]2020 Jan 31;18(1):26. PMID: 32005170 -
Theranostics
Proinflammatory macrophage-derived microvesicles exhibit tumor tropism dependent on CCL2/CCR2 signaling axis and promote drug delivery via SNARE-mediated membrane fusion. [Abstract]2020 May 17;10(15):6581-6598. PMID: 32550891 -
Theranostics
A Multifunctional Nanotherapy for Targeted Treatment of Colon Cancer by Simultaneously Regulating Tumor Microenvironment. [Abstract]2019 May 31;9(13):3732-3753. PMID: 31281510 -
Acta Pharm Sin B
Development of a bacteria-nanosapper for the active delivery of ZIF-8 particles containing therapeutic genes for cancer immune therapy. [Abstract]2024 Dec;14(12):5418-5434. PMID: 39807327
Amiloride hydrochloride dihydrate purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2024 Dec;14(12):5418-5434. [Abstract]
Amiloride hydrochloride (450 μM; 4 h) profoundly impaired the uptake rates of Il2/ZIF-8@Salmonella in RAW264.7 cells.
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Adv Sci (Weinh)
Lipid Droplet-Derived Biomimetic Nanocarriers for the Enhancement of Porcine Intermuscular Fat Content. [Abstract]2025 Jun;12(21):e2406150. PMID: 40365640 -
Adv Sci (Weinh)
Screening and Identification of Novel DNA Aptamer for Targeted Delivery to Injured Podocytes in Glomerular Diseases. [Abstract]2025 May;12(20):e2412356. PMID: 40178289 -
Adv Sci (Weinh)
Pharmaceutical Manipulation of Mitochondrial F0F1-ATP Synthase Enables Imaging and Protection of Myocardial Ischemia/Reperfusion Injury Through Stress-induced Selective Enrichment. [Abstract]2024 Mar;11(9):e2307880. PMID: 38093654
Amiloride hydrochloride dihydrate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Mar;11(9):e2307880. [Abstract]
Representative images and quantitative data of the NIR fluorescent signals in H9C2 cells exposed to IR‐780 pre‐treatment with ice condition, Amiloride (75 µg/mL; 30 min).
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Cell Rep Med
CLDN4 palmitoylation promotes hepatic-to-biliary lineage transition and lenvatinib resistance in hepatocellular carcinoma. [Abstract]2025 Jul 15;6(7):102208. PMID: 40592346 -
Biomaterials
Biodegradable polymers with tertiary amines enhance mRNA delivery of lipid nanoparticles via improved endosomal escape. [Abstract]2026 Jan:324:123541. PMID: 40644791 -
Biomaterials
Targeted immunomodulation therapy for cardiac repair by platelet membrane engineering extracellular vesicles via hitching peripheral monocytes. [Abstract]2022 May;284:121529. PMID: 35447403 -
J Control Release
Inducing potent ferroptosis in cancer cells by manipulation of ferritinophagy with a ferritin-targeted ATTEC. [Abstract]2026 Jan 10:389:114450. PMID: 41274571 -
J Control Release
2024 Dec:376:1316-1328. PMID: 39490420 -
Cell Mol Biol Lett
Acid-sensing ion channel-1 contributes to the failure of myelin sheath regeneration following spinal cord injury by transcellular delivery of PGE2. [Abstract]2024 Dec 3;29(1):149. PMID: 39627718 -
Environ Sci Technol
Graphene Quantum Dots Disrupt Embryonic Stem Cell Differentiation by Interfering with the Methylation Level of Sox 2. [Abstract]2021 Mar 2;55(5):3144-3155. PMID: 33569944 -
Small
Self-Assembling Cationic Lipopeptides for the Construction of Functional Vesicular Gene-Delivery Systems. [Abstract]2026 Jun 5:e74067. PMID: 42246404 -
Biosens Bioelectron
Cathepsin B dependent activatable trigger fluorophore (CAT-Fluor) for in situ functional imaging of antibody-drug conjugates. [Abstract]2025 Apr 15:274:117184. PMID: 39879789 -
Cell Commun Signal
Promoting AMPK/SR-A1-mediated clearance of HMGB1 attenuates chemotherapy-induced peripheral neuropathy. [Abstract]2023 May 4;21(1):99. PMID: 37143083 -
J Neuroinflammation
α-Synuclein-containing erythrocytic extracellular vesicles: essential contributors to hyperactivation of monocytes in Parkinson's disease. [Abstract]2022 Feb 22;19(1):53. PMID: 35193594 -
J Pharm Anal
Effect of cholesterol on distribution, cell uptake, and protein corona of lipid microspheres at sites of cardiovascular inflammatory injury. [Abstract]2025 Jul;15(7):101182. PMID: 40735031 -
Mater Today Bio
Metal-organic framework-based delivery systems as nanovaccine for enhancing immunity against porcine circovirus type 2. [Abstract]2025 Mar 27:32:101712. PMID: 40230641 -
J Hazard Mater
CYP450 activation and mitophagy induction mediate Ti3C2 MXene detoxification in RAW 264.7 cells. [Abstract]2025 Dec 5:500:140498. PMID: 41252987 -
Acta Biomater
Hydrogel system with growth cone-targeted hydroxyapatite nanorods: Regulating calcium signals for peripheral nerve injury repair. [Abstract]2025 Jul 1:201:171-186. PMID: 40419070 -
Food Chem
Absorption and transport mechanism of colloidal nanoparticles (CNPs) in lamb soup based on Caco-2 cell. [Abstract]2025 Jan 15;463(Pt 1):141196. PMID: 39260179 -
Proc Natl Acad Sci U S A
Endogenous ATP-powered nanomotors directing neural stem cell differentiation for Parkinson's disease treatment. [Abstract]2026 Mar 31;123(13):e2520119123. PMID: 41880568 -
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Int J Biol Macromol
Growth inhibition of pancreatic cancer by targeted delivery of gemcitabine via fucoidan-coated pH-sensitive liposomes. [Abstract]2024 Aug 5;277(Pt 3):134517. PMID: 39111497 -
Int J Biol Macromol
Polyethylenimine-coated PLGA nanoparticles containing Angelica sinensis polysaccharide promote dendritic cells activation and associated molecular mechanisms. [Abstract]2022 May 15:207:559-569. PMID: 35288164 -
Small Methods
2021 Apr;5(4):e2001047. PMID: 34927842 -
Cell Rep
LL-37 transports immunoreactive cGAMP to activate STING signaling and enhance interferon-mediated host antiviral immunity. [Abstract]2022 May 31;39(9):110880. PMID: 35649354 -
Br J Pharmacol
An orally available hypoglycaemic peptide taken up by caveolae transcytosis displays improved hypoglycaemic effects and body weight control in db/db mice. [Abstract]2020 Aug;177(15):3473-3488. PMID: 32293707 -
Anal Chem
Fluorogenic Peptide-Modulated 2D ZnO/MnO2 Nanosheet Heterostructure Enables Monitoring of Tumor Early Response to Immunotherapy. [Abstract]2026 Feb 10;98(5):3577-3587. PMID: 41603436 -
Anal Chem
Unveiling the Spatiotemporal Behavior of Chiral Quantum Dots During Cellular Endocytosis. [Abstract]2025 Jun 17;97(23):12026-12034. PMID: 40470594 -
J Ethnopharmacol
Screening bioactive compounds from Danggui-shaoyao-san for treating sodium retention in nephrotic syndrome using bio-affinity ultrafiltration. [Abstract]2022 Jun 28:292:115171. PMID: 35259444 -
J Cell Biol
2025 May 5;224(5):e202405060. PMID: 40136051 -
Transl Res
Employing the sustained-release properties of poly(lactic-co-glycolic acid) nanoparticles to reveal a novel mechanism of sodium-hydrogen exchanger 1 in neuropathic pain. [Abstract]2024 Jan:263:53-72. PMID: 37678757 -
Commun Biol
2022 Nov 14;5(1):1248. PMID: 36376440 -
J Zhejiang Univ Sci B
Pharmacological inhibition of ENaC or NCX can attenuate hepatic ischemia-reperfusion injury exacerbated by hypernatremia. [Abstract]2025 May 28;26(5):461-476. PMID: 40436642 -
mBio
2025 Mar 12;16(3):e0378724. PMID: 39964166 -
Mol Pharm
2025 Apr 7;22(4):2182-2192. PMID: 40032688 -
iScience
Renal lysophospholipase A1 contributes to Enterococcus faecalis-induced hypertension by enhancing sodium reabsorption. [Abstract]2022 Oct 19;25(12):105403. PMID: 36419851 -
FASEB J
Inhibition of ASIC1a reduces ferroptosis in rheumatoid arthritis articular chondrocytes via the p53/NRF2/SLC7A11 pathway. [Abstract]2025 Jan 15;39(1):e70298. PMID: 39760183 -
Microb Pathog
Antiviral effect of amiloride on replication of foot and mouth disease virus in cell culture. [Abstract]2019 Oct:135:103638. PMID: 31326561
Amiloride hydrochloride dihydrate purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2019 Oct:135:103638. [Abstract]
The viral mRNA and VP1 protein levels in IBRS-2 cells treated with different concentrations of Amiloride are determined by qPCR and Western Blot, respectively. ‘‘VC’’ indicates that the cells that are not treated with Amiloride.
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Nanotoxicology
Polystyrene nanoplastics promote tumor lung metastasis by inducing sodium overload in macrophages in an NMDAR-dependent way. [Abstract]2026 May 19:1-18. PMID: 42154607 -
J Leukoc Biol
Combination of microparticles vaccine with MSI-1436 exerts a strong immune response for hepatocellular carcinoma. [Abstract]2024 Sep 2;116(3):565-578. PMID: 39012079 -
J Pharm Biomed Anal
Screening method of mildronate and over 300 doping agents by reversed-phase liquid chromatography-high resolution mass spectrometry. [Abstract]2021 Feb 20:195:113870. PMID: 33453569 -
Nanotechnology
Construction and evaluation of BSA-CaP nanomaterials with enhanced transgene performance via biocorona-inspired caveolae-mediated endocytosis. [Abstract]2018 Feb 23;29(8):085101. PMID: 29256442 -
Cell Physiol Biochem
Characterization of a DNA Aptamer for Ovarian Cancer Clinical Tissue Recognition and in Vivo Imaging. [Abstract]2018;51(6):2564-2574. PMID: 30562733 -
Am J Transl Res
Knockdown of VDAC1 alleviates the cognitive dysfunction secondary to sepsis-associated encephalopathy. [Abstract]2021 Jul 15;13(7):7538-7555. PMID: 34377234 -
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NPJ Biomed Innov
2026 May 22;3(1):33. PMID: 42174191 -
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Sci Total Environ
2021 Jul 20:779:146523. PMID: 34030247
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 83.33 mg/mL (275.82 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 2 mg/mL (6.62 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
Animal Administration[1]
The experiments are performed on male and female Sprague-Dawley rats that have been maintained in a heat controlled room maintained at 23° C with a 12h/12h light-dark schedule. A total of 116 adult rats are injected i.p. with Amiloride hydrochloride dihydrate (0.1, 0.5, and 2.0 mg/kg). After 15, 30, 45, or 120 min post-injection, the rats are anesthetized with 8% chloral hydrate, the chest is opened, and blood samples are obtained by cardiac puncture. The blood is centrifuged at 5000 RPM for 5 min, and the plasma (1.5 mL) separated and stored at -80° C[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Reinheit & Dokumentation
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Data Sheet (278 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Miller RL, et al. Blockade of ENaCs by amiloride induces c-Fos activation of the area postrema. Brain Res. 2015 Mar 19;1601:40-51. [Content Brief]
[2]. Xu LB, et al. Amiloride, a urokinase-type plasminogen activator receptor (uTPA) inhibitor, reduces proteinurea in podocytes. Genet Mol Res. 2015 Aug 14;14(3):9518-29. [Content Brief]
[3]. Giamarchi A, et al. A polycystin-2 (TRPP2) dimerization domain essential for the function of heteromeric polycystin complexes. EMBO J. 2010 Apr 7;29(7):1176-91. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.3099 mL | 16.5497 mL | 33.0994 mL | 82.7486 mL |
| 5 mM | 0.6620 mL | 3.3099 mL | 6.6199 mL | 16.5497 mL | |
| DMSO | 10 mM | 0.3310 mL | 1.6550 mL | 3.3099 mL | 8.2749 mL |
| 15 mM | 0.2207 mL | 1.1033 mL | 2.2066 mL | 5.5166 mL | |
| 20 mM | 0.1655 mL | 0.8275 mL | 1.6550 mL | 4.1374 mL | |
| 25 mM | 0.1324 mL | 0.6620 mL | 1.3240 mL | 3.3099 mL | |
| 30 mM | 0.1103 mL | 0.5517 mL | 1.1033 mL | 2.7583 mL | |
| 40 mM | 0.0827 mL | 0.4137 mL | 0.8275 mL | 2.0687 mL | |
| 50 mM | 0.0662 mL | 0.3310 mL | 0.6620 mL | 1.6550 mL | |
| 60 mM | 0.0552 mL | 0.2758 mL | 0.5517 mL | 1.3791 mL | |
| 80 mM | 0.0414 mL | 0.2069 mL | 0.4137 mL | 1.0344 mL | |
| 100 mM | 0.0331 mL | 0.1655 mL | 0.3310 mL | 0.8275 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Amiloride hydrochloride dihydrate Related Classifications
HY-B0285B Related Classifications
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