Art. -Nr.: HY-19987

BML-284

Name: BML-284
Brand: MedChemExpress
SKU: HY-19987
Price: 70.00 USD
Availability: InStock
Rating: 5 (43 reviews)

43 Cited Publications

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Based on 43 publication(s) in Google Scholar

BML-284 is a potent and cell-permeable Wnt signaling activator. BML-284 induces TCF-dependent transcriptional activity with an EC₅₀ of 700 nM.

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Reinheit : 99.99%
CAS. Nr.: 853220-52-7
Formel: C₁₉H₁₈N₄O₃
Molecular Weight:350.37

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

BML-284 Chemical Structure

CAS. Nr.: 853220-52-7

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (43)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

BML-284 is a potent and cell-permeable Wnt signaling activator. BML-284 induces TCF-dependent transcriptional activity with an EC₅₀ of 700 nM^[1].

IC₅₀ & Target

EC50: 700 nM (TCF-dependent transcriptional activity)^[1]

Alle Wnt Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	0.52 μM Compound: BML284	Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability measured after 72 hrs Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability measured after 72 hrs	[PMID: 38430854]
HeLa	IC₅₀	0.09 μM Compound: BML284	Antiproliferative activity against human HeLa cells assessed as reduction in cell viability measured after 72 hrs Antiproliferative activity against human HeLa cells assessed as reduction in cell viability measured after 72 hrs	[PMID: 38430854]
K562	IC₅₀	0.1 μM Compound: BML284	Antiproliferative activity against human K562 cells assessed as reduction in cell viability measured after 72 hrs Antiproliferative activity against human K562 cells assessed as reduction in cell viability measured after 72 hrs	[PMID: 38430854]
MCF7	IC₅₀	0.69 μM Compound: BML284	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs	[PMID: 38430854]
MOLM-13	IC₅₀	0.19 μM Compound: BML284	Antiproliferative activity against human MOLM-13 cells assessed as reduction in cell viability measured after 72 hrs Antiproliferative activity against human MOLM-13 cells assessed as reduction in cell viability measured after 72 hrs	[PMID: 38430854]

In Vitro

BML-284 (10 μM; 24 hours) significantly increases the migration and invasion of both MNK45 and AGS cells and partially restores the migratory and invasive abilities of cells inhibited by pizotifen (HY-B0115)^[1].
BML-284 (10 μM; 24 hours) induces the expression of β-catenin significantly when compared with the NC group. It also partially reverses the effects induced by pizotifen on E-cadherin and N-cadherin expression in MNK45 and AGS cells compared with the pizotifen-treated group^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

Human gastric cancer cell lines MNK45 and AGS^[1]
Concentration:

10 µM
Incubation Time:

24 hours
Result:

Induced β-catenin expression and reserved E-cadherin and N-cadherin expression in MNK45 and AGS cells.

In Vivo

BML-284 (10 ng) combines with Pyrimethanil (4 mg/L) could partially rescue the malformed phenotype and cardiac defects induced by Pyrimethanil in Tg (myl7:EGFP) transgenic embryos at 5.5 hpf are transferred into plates with 20 embryos^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 853220-52-7
Appearance Solid
Molecular Weight 350.37
Formel C₁₉H₁₈N₄O₃
Color White to yellow
SMILES

NC1=NC(C2=CC=CC(OC)=C2)=CC(NCC3=CC=C(OCO4)C4=C3)=N1
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (43)

All WBCell Imaging/StainingRT-PCRIFIn Vivo Efficacy Study

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Journal Impact Factor

Sci Adv

CLIC5 promotes myoblast differentiation and skeletal muscle regeneration via the BGN-mediated canonical Wnt/β-catenin signaling pathway. [Abstract]

2024 Oct 11;10(41):eadq6795. PMID: 39999205
Adv Healthc Mater

Bioinspired Microgel Assembly of Liver-Derived Decellularized Extracellular Matrix Enhances Dentin-Pulp Regeneration via Stemness Awakening. [Abstract]

2026 Jan 4:e03543. PMID: 41486849
J Hazard Mater

Insights into the circadian rhythm alterations of the novel PFOS substitutes F-53B and OBS on adult zebrafish. [Abstract]

2023 Apr 15:448:130959. PMID: 36860044
Cell Death Discov

Suppressed Akt/GSK-3β/β-catenin signaling contributes to excessive adipogenesis of fibro-adipogenic progenitors after rotator cuff tears. [Abstract]

2023 Aug 25;9(1):312. PMID: 37626040

BML-284 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]

Protein level of β-catenin, PPARγ, and GAPDH for RCT-FAPs (FAPs isolated from supraspinatus muscle one week after tendon tears) with or without β-catenin agonist BML-284 (1 μM) treatment.

BML-284 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]

The oil red staining and statistical analysis of RCT-FAPs after 7-day adipogenic induction in AIM with DMSO or BML-284 (1 μM).

BML-284 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]

Relative mRNA expression level of adipogenic genes for RCT-FAPs after 7-day adipogenic induction in AIM with DMSO or BML-284 (1 μM).

BML-284 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]

Immunofluorescence staining of perilipin1 for supraspinatus muscles from the DMSO group and BML-284 (1 μM) group.

BML-284 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]

Gross appearance of supraspinatus muscles from the DMSO and BML-284 (1 μM) treatment groups.
Proc Natl Acad Sci U S A

Calcineurin controls proximodistal blastema polarity in zebrafish fin regeneration. [Abstract]

2021 Jan 12;118(2):e2009539118. PMID: 33376206
Free Radic Biol Med

Triphenyltin chloride suppresses caudal fin regeneration in zebrafish by inhibiting Wnt signaling. [Abstract]

2025 Nov 21:243:231-244. PMID: 41275934
Cell Rep

Astrocyte-derived CHI3L1 signaling impairs neurogenesis and cognition in the demyelinated hippocampus. [Abstract]

2024 May 10;43(5):114226. PMID: 38733586
Ecotoxicol Environ Saf

Ethoprophos induces cardiac toxicity in zebrafish embryos. [Abstract]

2021 Nov 27:228:113029. PMID: 34847436
Ecotoxicol Environ Saf

Cardiac toxicity assessment of pendimethalin in zebrafish embryos. [Abstract]

2021 Oct 1:222:112514. PMID: 34280841
J Environ Sci

2026 May 9.
Eur J Pharmacol

Melatonin suppresses cellular senescence in diabetic kidney disease though the Wnt/β-catenin signaling pathway. [Abstract]

2026 Feb 15:1015:178541. PMID: 41534557
Biol Direct

Inhibition of PRC1 elicits immunogenic cell death by triggering ROS-dependent ER stress in colorectal cancer via the Wnt/β-catenin signaling pathway. [Abstract]

2025 Aug 22;20(1):94. PMID: 40847353
Biochim Biophys Acta Mol Basis Dis

Glioma cells produce soluble PD-L1 via the Wnt/β-catenin signaling pathway to suppress CD8+ T cell activity. [Abstract]

2025 Aug 8;1871(8):168013. PMID: 40784599
Mol Med Rep

Pigment epithelium‑derived factor inhibits proliferation, invasion and angiogenesis, and induces ferroptosis of extravillous trophoblasts by targeting Wnt‑β‑catenin/VEGF signaling in placenta accreta spectrum. [Abstract]

2024 May;29(5):75. PMID: 38488028
Sci Rep

Cardamonin inhibits the growth and stemness of osteosarcoma stem cells by inducing autophagy and inhibiting the Wnt/β-catenin signaling pathway. [Abstract]

2025 Aug 12;15(1):29484. PMID: 40796594
Am J Pathol

Insulin Promotes Corneal Nerve Repair and Wound Healing in Type 1 Diabetic Mice by Enhancing Wnt/β-Catenin Signaling. [Abstract]

2020 Nov;190(11):2237-2250. PMID: 32858016
Prog Neurobiol

Astrocyte-secreted C3 signaling impairs neuronal development and cognition in autoimmune diseases. [Abstract]

2024 Sep:240:102654. PMID: 38945516
Comp Biochem Physiol C Toxicol Pharmacol

2-Hydroxyanthraquinone exposure causes the damage of cerebrovascular and blood brain barrier in zebrafish via inducing inflammation and downregulation of the Wnt/β-catenin signaling pathway. [Abstract]

2026 Mar:301:110432. PMID: 41386556
J Cell Physiol

Identification of key events and regulatory networks in the formation process of primordial germ cell based on proteomics. [Abstract]

2023 Mar;238(3):610-630. PMID: 36745473
Genomics

PLOD1 promote proliferation and migration with glycolysis via the Wnt/β-catenin pathway in THCA. [Abstract]

2024 Nov;116(6):110943. PMID: 39424162
Exp Cell Res

NOP14 promotes proliferative diabetic retinopathy through ribosome biogenesis and endothelial dysfunction via Wnt/β-catenin signaling activation. [Abstract]

2026 Apr 1;457(1):114922. PMID: 41638389
Cell Biol Int

RFC4 Promotes the Metastasis of Colorectal Cancer by Regulating the Wnt/β-Catenin Pathway. [Abstract]

2026 Feb;50(2):e70136. PMID: 41574400
Mol Carcinog

TRIM16 E121D variant affects the risk and prognosis of hepatocellular carcinoma by modulating the Wnt/β-catenin pathway. [Abstract]

2023 Nov;62(11):1686-1699. PMID: 37477507
Food Chem Toxicol

Copper exposure induced developmental toxicity and cardiotoxicity in zebrafish. [Abstract]

2025 Oct:204:115660. PMID: 40706976
Adv Biol (Weinh)

IER3 Facilitates Tumor Progression and Aberrant Glycolysis via Activating wnt/β-Catenin Pathway in Oral Squamous Cell Carcinoma. [Abstract]

2025 Jul;9(7):e2400564. PMID: 40128149
Mol Biotechnol

Nucleoporin 93 Regulates Cancer Cell Growth and Stemness in Bladder Cancer via Wnt/β-Catenin Signaling. [Abstract]

2025 May;67(5):2072-2084. PMID: 38744786
Discov Oncol

PTEN inhibits epithelial mesenchymal transition of thyroid cancer cells by regulating the Wnt/β-Catenin signaling pathway. [Abstract]

2024 Dec 18;15(1):803. PMID: 39692895
Exp Ther Med

Pizotifen inhibits the proliferation and invasion of gastric cancer cells. [Abstract]

2020 Feb;19(2):817-824. PMID: 32010241
In Vitro Cell Dev Biol Anim

A1CF-Axin2 signal axis regulates apoptosis and migration in Wilms tumor-derived cells through Wnt/β-catenin pathway. [Abstract]

2019 Apr;55(4):252-259. PMID: 30825095
Biol Pharm Bull

Loureirin A Promotes Cell Differentiation and Suppresses Migration and Invasion of Melanoma Cells via WNT and AKT/mTOR signaling Pathways. [Abstract]

2024 Feb 22;47(2):486-498. PMID: 38199251
Biol Pharm Bull

Emodin Suppresses the Migration and Invasion of Melanoma Cells. [Abstract]

2021 Jun 1;44(6):771-779. PMID: 33731543
Acta Cir Bras

miR-29c-3p represses the angiogenesis of esophageal squamous cell carcinoma by targeting SERPINH1 to regulate the Wnt signaling pathway. [Abstract]

2023 Dec 4:38:e385223. PMID: 38055382
Mol Cell Toxicol

2024 Oct 31.
Egypt J Med Hum Genet

2025 Dec 22;26(1):189.
Acta Orthop Belg

CTHRC1 inhibits the proliferation and promotes apoptosis of osteoarthritis chondrocytes by activating Wnt / ß-Catenin pathway. [Abstract]

2026 Apr 20;92(1). PMID: 42012045
Seoul National University

2032.
Environ Toxicol

Tescalcin modulates bone marrow-derived mesenchymal stem cells osteogenic differentiation via the Wnt/β-catenin signaling pathway. [Abstract]

2024 Mar;39(3):1836-1846. PMID: 38124301
Research Square Preprint

2023 May 17.
Research Square Print

2023 Mar 13.
Sci Total Environ

Hematopoietic stem cell and immunotoxicity in zebrafish embryos induced by exposure to Metalaxyl-M. [Abstract]

2022 Feb 25;809:152102. PMID: 34863748
Eur Rev Med Pharmacol Sci

MSCs reduce airway remodeling in the lungs of asthmatic rats through the Wnt/β-catenin signaling pathway. [Abstract]

2020 Nov;24(21):11199-11211. PMID: 33215438
Chemosphere

Exposure to pyrimethanil induces developmental toxicity and cardiotoxicity in zebrafish. [Abstract]

2020 Sep:255:126889. PMID: 32388256
ACS Comb Sci

Benzimidazolyl-pyrazolo[3,4- b]pyridinones, Selective Inhibitors of MOLT-4 Leukemia Cell Growth and Sea Urchin Embryo Spiculogenesis: Target Quest. [Abstract]

2019 Dec 9;21(12):805-816. PMID: 31689077

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : ≥ 100 mg/mL (285.41 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8541 mL

14.2706 mL

28.5413 mL

5 mM

0.5708 mL

2.8541 mL

5.7083 mL

10 mM

0.2854 mL

1.4271 mL

2.8541 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Liu J, et al. A small-molecule agonist of the Wnt signaling pathway. Angew Chem Int Ed Engl. 2005 Mar 18;44(13):1987-90. [Content Brief]

[2]. Ying Jiang, et al. Pizotifen inhibits the proliferation and invasion of gastric cancer cells. Exp Ther Med. 2020 Feb;19(2):817-824. [Content Brief]

[3]. Yunlong Meng, et al. Exposure to pyrimethanil induces developmental toxicity and cardiotoxicity in zebrafish. Chemosphere. 2020 Sep;255:126889. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8541 mL	14.2706 mL	28.5413 mL	71.3531 mL
	5 mM	0.5708 mL	2.8541 mL	5.7083 mL	14.2706 mL
	10 mM	0.2854 mL	1.4271 mL	2.8541 mL	7.1353 mL
	15 mM	0.1903 mL	0.9514 mL	1.9028 mL	4.7569 mL
	20 mM	0.1427 mL	0.7135 mL	1.4271 mL	3.5677 mL
	25 mM	0.1142 mL	0.5708 mL	1.1417 mL	2.8541 mL
	30 mM	0.0951 mL	0.4757 mL	0.9514 mL	2.3784 mL
	40 mM	0.0714 mL	0.3568 mL	0.7135 mL	1.7838 mL
	50 mM	0.0571 mL	0.2854 mL	0.5708 mL	1.4271 mL
	60 mM	0.0476 mL	0.2378 mL	0.4757 mL	1.1892 mL
	80 mM	0.0357 mL	0.1784 mL	0.3568 mL	0.8919 mL
	100 mM	0.0285 mL	0.1427 mL	0.2854 mL	0.7135 mL

Bioz

Keywords

BML-284

43 Cited Publications

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BML-284

Publications Citing Use of MedChemExpress (MCE) BML-284

Alle Wnt Isoform-spezifische Produkte anzeigen