Art. -Nr.: HY-12990

Spautin-1

24 Cited Publications

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Based on 24 publication(s) in Google Scholar

Spautin-1 is a specific and potent autophagy inhibitor which inhibits ubiquitin-specific peptidases, USP10 and USP13 with IC₅₀s of 0.6-0.7 μM.

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Reinheit: 99.54%
CAS. Nr.: 1262888-28-7
Formel: C₁₅H₁₁F₂N₃
Molecular Weight:271.26

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Spautin-1 Chemical Structure

CAS. Nr.: 1262888-28-7

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (24)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Spautin-1 is a specific and potent autophagy inhibitor which inhibits ubiquitin-specific peptidases, USP10 and USP13 with IC₅₀s of 0.6-0.7 μM.

IC₅₀ & Target

USP10

0.6-0.7 μM (IC₅₀)

Alle Deubiquitinase Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
MIA PaCa-2	EC₅₀	20 μM Compound: Spautin-1	Antiproliferative activity against human MIA PaCa-2 cells assessed as cell viability measured after 72 hrs by MTT assay Antiproliferative activity against human MIA PaCa-2 cells assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 28105281]
PC-9	IC₅₀	>5 μM Compound: 5	Cytotoxicity against human PC-9 cells harboring EGFR mutant assessed as inhibition of cell growth incubated for 72 hrs by ATP-based luminescence CellTiter-Glo assay Cytotoxicity against human PC-9 cells harboring EGFR mutant assessed as inhibition of cell growth incubated for 72 hrs by ATP-based luminescence CellTiter-Glo assay	[PMID: 36410591]

In Vitro

Spautin-1 enhances imatinib mesylate (IM)-induced CmL cell apoptosis by reducing the expression of the anti-apoptotic proteins Mcl-1 and Bcl-2. The pro-apoptotic activity of spautin-1 is associated with activation of GSK3β, an important downstream effector of PI3K/AKT. Spautin-1 enhances IM-induced cytotoxicity in CmL cell line K562, decreasing the IC₅₀ from 1 to 0.5 μM^[1]. The mechanism of spautin-1 acting on acute pancreatitis is associated with impaired autophagy inhibition^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Spautin-1 ameliorates the pathogenesis of acute pancreatitis induced by cerulein or L-arginine. Spautin-1 pretreatment significantly diminishes the elevation of serum amylase and lipase levels, which are indicative of trypsin activity. Increasing levels of serum TNFα caused by cerulein are inhibited in the presence of spautin-1. Spautin-1 treatment can ameliorate the inflammation damage induced by cerulein, such as edema, degeneration, coagulative necrosis and infiltration of inflammatory cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 1262888-28-7
Appearance Solid
Molecular Weight 271.26
Formel C₁₅H₁₁F₂N₃
Color White to light yellow
SMILES

FC1=CC2=C(N=CN=C2C=C1)NCC3=CC=C(F)C=C3
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (24)

All IHCWBIPCell Proliferation/Viability AssayHistological Imaging/StainingIF

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Journal Impact Factor

ACS Nano

Emerging of Ultrafine Membraneless Organelles as the Missing Piece of Nanostress: Mechanism of Biogenesis and Implications at Multilevels. [Abstract]

2025 Feb 11;19(5):5659-5679. PMID: 39882824

Spautin-1 purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Feb 11;19(5):5659-5679. [Abstract]

TAK-243/Spautin-1 groups results consistently support a positive correlation between the activity of HSP90, ubiquitination, and proteasome system with the disassembly of NSGs.
Nat Commun

Nanomaterial signatures program biomolecular condensates via triphasic separation for chemoplasticity remodeling. [Abstract]

2025 Oct 29;16(1):9554. PMID: 41162367
Nat Commun

USP2 inhibition unleashes CD47-restrained phagocytosis and enhances anti-tumor immunity. [Abstract]

2025 May 16;16(1):4564. PMID: 40379682
Nat Commun

USP8 inhibition reshapes an inflamed tumor microenvironment that potentiates the immunotherapy. [Abstract]

2022 Mar 31;13(1):1700. PMID: 35361799
Cell Death Differ

Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma. [Abstract]

2023 Feb;30(2):527-543. PMID: 36526897

Spautin-1 purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2023 Feb;30(2):527-543. [Abstract]

ESCC cells were treated with different concentrations of Spautin-1 (0, 20, 40, 80 μM) for 24 h, and then western blotting was used to detect the protein levels of ANLN and USP10.
Acta Pharm Sin B

2025 Nov 13.
Sci Adv

USP13 stabilizes NLRP3 to facilitate inflammasome activation by preventing TRIM31-mediated NLRP3 ubiquitination and degradation. [Abstract]

2025 Sep 26;11(39):eadx3827. PMID: 41004574

Spautin-1 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Sep 26;11(39):eadx3827. [Abstract]

HEK293T cells transfected with the indicated plasmid combinations were treated with Spautin-1 (10 μM) for 6 hours, Before collection, cells were treated with MG132 for 6 hours. Immunoblot analysis of NLRP3 ubiquitination in cell lysates immunoprecipitated with anti–c-Myc beads.
Redox Biol

USP20 governs tyrosine kinase inhibitors resistance through ferroptosis evasion by targeting GPX4 in cancers. [Abstract]

2026 May:92:104086. PMID: 41844497
Biochem Pharmacol

Discovery of targeting USP10-mediated proline metabolism arrangement to inhibit hepatocellular carcinoma progression. [Abstract]

2025 Mar 28:116904. PMID: 40158816
Cancer Immunol Immunother

Spautin-1 inhibits the growth of diffuse large B-cell lymphoma by inducing mitochondrial damage-mediated PANoptosis and anti-tumor immunity. [Abstract]

2025 Aug 25;74(9):293. PMID: 40853486

Spautin-1 purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2025 Aug 25;74(9):293. [Abstract]

Cell viability of SU-DHL-4, SU-DHL-6, OCI-LY3, OCI-Ly10, and A20 cell lines detected by a CCK-8 kit after Spautin-1 (0.1, 0.3, 1, 3, 10, 30, 100 μM, 24, 48 h) treatment for the indicated time.

Spautin-1 purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2025 Aug 25;74(9):293. [Abstract]

H&E staining showed that Spautin-1 (20 mg/kg, i.p.) induced tumor cell shrinkage, fragmentation, and disorganized tissue architecture.

Spautin-1 purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2025 Aug 25;74(9):293. [Abstract]

Spautin-1 Spautin-1 (20 mg/kg, i.p.) treatment induced the infiltration of CD3⁺/CD4⁺ and CD8⁺ T cells into the tumor tissues.
Int J Mol Sci

Deubiquitinating Enzymes Ubiquitin-Specific Proteases 7 and 10 Regulate TAU Aggregation. [Abstract]

2025 Nov 15;26(22):11062. PMID: 41303552
Int Immunopharmacol

Baicalein limits subchondral bone lesions via AMPKα/BECN1 activation in osteoarthritis osteoblast. [Abstract]

2026 Apr 15:175:116446. PMID: 41791307
Biochim Biophys Acta Mol Basis Dis

Deficiency in the conserved ECHS1 gene causes Leigh syndrome by impairing mitochondrial respiration efficiency and suppressing ADRB2-PKA signaling. [Abstract]

2025 May 28:167930. PMID: 40446940
Biochim Biophys Acta Mol Basis Dis

Ubiquitin-specific peptidase 10 promotes renal interstitial fibrosis progression through deubiquitinating and stabilizing P53 protein. [Abstract]

2025 Jan 7:167660. PMID: 39788218
Biochim Biophys Acta Mol Basis Dis

USP13 inhibition exacerbates mitochondrial dysfunction and acute kidney injury by acting on MCL-1. [Abstract]

2024 Nov 26:167599. PMID: 39608596
J Cell Mol Med

Inhibition of autophagy-dependent pyroptosis attenuates cerebral ischaemia/reperfusion injury. [Abstract]

2021 Jun;25(11):5060-5069. PMID: 33938129
iScience

Exosomes derived from 3D-cultured hUCMSCs exhibit superior hepatoprotection against ALF. [Abstract]

2025 Aug 21;28(9):113419. PMID: 40978151
Microbiol Spectr

Identification of In Vitro Inhibitors of Monkeypox Replication. [Abstract]

2023 Aug 17;11(4):e0474522. PMID: 37278625
Life Metab

Glucose-6-phosphate dehydrogenase regulates mitophagy by maintaining PINK1 stability. [Abstract]

2024 Dec 13;4(1):loae040. PMID: 39872984
Cancer Res Commun

Autophagy inhibition-induced cytosolic DNA sensing combined with differentiation therapy induces irreversible myeloid differentiation in leukemia cells. [Abstract]

2024 Mar 20;4(3):849-860. PMID: 38466568
Cancer Med

CaMKII Exacerbates Doxorubicin-Induced Cardiotoxicity by Promoting Ubiquitination Through USP10 Inhibition. [Abstract]

2024 Nov;13(21):e70286. PMID: 39517125
bioRxiv

Antivirals against monkeypox infections. [Abstract]

2023 Apr 19:2023.04.19.537483. PMID: 37131608
Research Square Print

January 4th, 2023.
Research Square Print

September 14th, 2022.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (184.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.6865 mL

18.4325 mL

36.8650 mL

5 mM

0.7373 mL

3.6865 mL

7.3730 mL

10 mM

0.3687 mL

1.8433 mL

3.6865 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (9.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (9.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% PBS

Solubility: 10 mg/mL (36.87 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

Cell Assay^[1]

Spautin-1 is dissolved in DMSO. Cell proliferation is evaluated using CCK-8 kit. K562 cells (1x10⁵/mL) are seeded into 96-well plates in triplicate and then treated with 125 to 4,000 nM IM alone or in combi¬nation with spautin-1 (10 μM). After 48 h of incubation, 10 μL of CCK-8 reagent is added to each well. Four hours later, the absorbance is read at 450 nm using a microplate reader^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[2]

Mice: In this study, mice models with acute pancreatitis, including cerulein- and L-arginine-induced models, are constructed. For the cerulein-induced model, four intraperitoneal injections of cerulein (50 μg/kg body weight) are given consecutively at hourly intervals; The L-arginine-induced model received hourly intraperitoneal injections of 1.4 g/kg (optimal dosage for this study) L-arginine three times^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Shao S, et al. Spautin-1, a novel autophagy inhibitor, enhances imatinib-induced apoptosis in chronic myeloid leukemia. Int J Oncol. 2014 May;44(5):1661-1668. [Content Brief]

[2]. Xiao J, et al. Spautin-1 Ameliorates Acute Pancreatitis via Inhibiting Impaired Autophagy and Alleviating Calcium Overload. Mol Med. 2016 Aug 18;22. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.6865 mL	18.4325 mL	36.8650 mL	92.1625 mL
	5 mM	0.7373 mL	3.6865 mL	7.3730 mL	18.4325 mL
	10 mM	0.3686 mL	1.8432 mL	3.6865 mL	9.2162 mL
	15 mM	0.2458 mL	1.2288 mL	2.4577 mL	6.1442 mL
	20 mM	0.1843 mL	0.9216 mL	1.8432 mL	4.6081 mL
	25 mM	0.1475 mL	0.7373 mL	1.4746 mL	3.6865 mL
	30 mM	0.1229 mL	0.6144 mL	1.2288 mL	3.0721 mL
	40 mM	0.0922 mL	0.4608 mL	0.9216 mL	2.3041 mL
	50 mM	0.0737 mL	0.3686 mL	0.7373 mL	1.8432 mL
	60 mM	0.0614 mL	0.3072 mL	0.6144 mL	1.5360 mL
	80 mM	0.0461 mL	0.2304 mL	0.4608 mL	1.1520 mL
	100 mM	0.0369 mL	0.1843 mL	0.3686 mL	0.9216 mL

Keywords

Spautin-1

24 Cited Publications

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Spautin-1

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Ubiquitination Compound Library

Metabolic Enzyme Compound Library

Protease Inhibitor Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) Spautin-1

Alle Deubiquitinase Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (24)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Spautin-1

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Ubiquitination Compound Library

Metabolic Enzyme Compound Library

Protease Inhibitor Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) Spautin-1

Alle Deubiquitinase Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (24)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Spautin-1 Related Classifications

HY-12990 Related Classifications