Okanin
Based on 3 publication(s) in Google Scholar
Okanin, effective constituent of the flower tea Coreopsis tinctoria, attenuates LPS-induced microglial activation through inhibition of the TLR4/NF-κB signaling pathways.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.76%
- CAS. Nr.: 484-76-4
- Formel: C15H12O6
- Molecular Weight:288.25
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Speicherung:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) Okanin
More
Customer Validation & Images
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Histological Imaging/Staining
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WB
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ELISA
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RT-PCR
Biologische Aktivität
Beschreibung
IC50 & Target
[1]|
TLR4 |
p65 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
6 μM
Compound: 5a
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Inhibition of human telomerase from HEK293 cell extracts by Flash-Plate assay
Inhibition of human telomerase from HEK293 cell extracts by Flash-Plate assay
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[PMID: 15588081] |
In Vitro
Okanin significantly inhibits LPS-induced TLR4 expression in BV-2 cells. Okanin inhibits the translocation of the NF-κB p65 subunit from the cytosol to the nucleus. Okanin significantly suppresses LPS-induced iNOS expression and also inhibites IL-6 and TNF-α production and mRNA expression in LPS-stimulated BV-2 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS. Nr. 484-76-4
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Appearance Solid
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Molecular Weight 288.25
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Formel C15H12O6
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Color Yellow to brown
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SMILES
O=C(C1=CC=C(O)C(O)=C1O)/C=C/C2=CC=C(O)C(O)=C2
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (3)
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Journal Impact Factor
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Most Recent
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Dis Markers
LTB4 Promotes Acute Lung Injury via Upregulating the PLC ε-1/TLR4/NF- κ B Pathway in One-Lung Ventilation. [Abstract]2022 Jan 11:2022:1839341. PMID: 35059042
Okanin purchased from MedChemExpress. Usage Cited in: Dis Markers. 2022 Jan 11:2022:1839341. [Abstract]
H&E staining examined the pathology of lung tissues in control rats, OLV rats, PLCε-1 knockdown rats, and Okanin (50 mg/kg, i.p.)-treated rats.
Okanin purchased from MedChemExpress. Usage Cited in: Dis Markers. 2022 Jan 11:2022:1839341. [Abstract]
Western blot detected the protein levels of Bcl-2, Bax, caspase 3 in control rats, OLV rats, PLCε-1 knockdown rats, and Okanin (50 mg/kg, i.p.)-treated rats.
Okanin purchased from MedChemExpress. Usage Cited in: Dis Markers. 2022 Jan 11:2022:1839341. [Abstract]
ELISA performed to examine the levels of inflammation mediators GM-CSF and IL-1β ) in control rats, OLV rats, PLCε-1 knockdown rats, and Okanin (50 mg/kg, i.p.)-treated rats.
Okanin purchased from MedChemExpress. Usage Cited in: Dis Markers. 2022 Jan 11:2022:1839341. [Abstract]
qRT-PCR validated expression of LTA4H, BLT1, and PLCε-1 in control rats, OLV rats, PLCε-1 knockdown rats, and Okanin (50 mg/kg, i.p.)-treated rats.
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 16.67 mg/mL (57.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.67 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
Reinheit & Dokumentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4692 mL | 17.3461 mL | 34.6921 mL | 86.7303 mL |
| 5 mM | 0.6938 mL | 3.4692 mL | 6.9384 mL | 17.3461 mL | |
| 10 mM | 0.3469 mL | 1.7346 mL | 3.4692 mL | 8.6730 mL | |
| 15 mM | 0.2313 mL | 1.1564 mL | 2.3128 mL | 5.7820 mL | |
| 20 mM | 0.1735 mL | 0.8673 mL | 1.7346 mL | 4.3365 mL | |
| 25 mM | 0.1388 mL | 0.6938 mL | 1.3877 mL | 3.4692 mL | |
| 30 mM | 0.1156 mL | 0.5782 mL | 1.1564 mL | 2.8910 mL | |
| 40 mM | 0.0867 mL | 0.4337 mL | 0.8673 mL | 2.1683 mL | |
| 50 mM | 0.0694 mL | 0.3469 mL | 0.6938 mL | 1.7346 mL |
Okanin Related Classifications
HY-N6673 Related Classifications
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