Cat. No.: HY-17565

Bleomycin sulfate

(Synonyms: Bleomycin sulfate)

208 Cited Publications

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Based on 208 publication(s) in Google Scholar

Bleomycin sulfate is a DNA synthesis inhibitor. Bleomycin hydrochloride is a DNA damaging agent. Bleomycin sulfate is an antitumor antibiotic.

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Pureté: 99.90%
CAS No.: 9041-93-4
Masse moléculaire:1523 (average)

Stockage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Bleomycin sulfate Chemical Structure

CAS No.: 9041-93-4

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Induced Disease Models
Chemical Information
Publications (208)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Bleomycin sulfate is a DNA synthesis inhibitor. Bleomycin hydrochloride is a DNA damaging agent. Bleomycin sulfate is an antitumor antibiotic^[1].

IC₅₀ & Target

DNA/RNA Synthesis^[1]

Voir tous les produits spécifiques à Isoform DNA/RNA Synthesis :

Voir tous les produits spécifiques à Isoform Antibiotic :

In Vitro

Bleomycin (BLM) sulfate is chosen as the best-studied micronucleus (MN) inducers in human lymphocytes with different mechanisms of genotoxicity. The most frequent Bleomycin-induced DNA lesions are single and double strand breaks and single apuinic/apyrimidinic sites. At the same time Bleomycin is true radiomimetic compound, resembling almost completely the genetic effect of ionizing radiation^[1].
The IC₅₀ value of Bleomycin sulfate for UT-SCC-19A cell line is 4.0±1.3 nM. UT-SCC-12A and UT-SCC-12B are both more resistant to Bleomycin (BLM); IC₅₀ values are 14.2±2.8 nM and 13.0±1.1 nM, respectively^[2].
Bleomycin sulfate (50, 100 μM; for 24, 48 h) induce pulmonary fibrosis in RLE-6TN cell (50 μM) and A549 cell (100 μM)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Animal Model:

Male Fischer 344 rats, 8-10 week old, weighing 150-250 g^[3]
Dosage:

3.5-4 mg/kg
Administration:

Intra-tracheal
Result:

Body weights decreased by day 4 then increased by Day 7 through the end of the study.

Induced Disease Models

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Bleomycin sulfate can be used to induce pulmonary fibrosis models. After administration via intramuscular, subcutaneous, intraperitoneal, or intrapleural routes, Bleomycin sulfate is rapidly absorbed and reaches peak plasma concentrations in approximately 60 minutes. Less than 1% of intravenously injected Bleomycin sulfate binds to plasma proteins, ensuring high bioavailability. The mean plasma clearance rate of Bleomycin sulfate is about 70 mL/min/m², indicating high plasma elimination and urinary excretion rates²^[6].

Induction of Pulmonary Fibrosis^[7]

Background

Bleomycin sulfate can lead to lung patchy parenchymal inflammation, epithelial cell injury with reactive hyperplasia, epithelial-mesenchymal transition, activation and differentiation of fibroblasts to myofibroblasts, and basement membrane and alveolar epithelium injures. The experimental use of Bleomycin sulfate is to induce pulmonary fibrosis animal models.

Specific Modeling Methods

Mice: C57BL/6 • 12-week-old
Administration: 3-5 mg/kg • intratracheal administration • sprays on day one

Note

The mice were housed in separate stainless-steel cages (six mice per cage) in a temperature-controlled environment (20-24°C) on 12 h light-dark cycles with unrestricted access to food and water.

Modeling Indicators

Body quality changes: The appetite activity is reduced, with the fur less shiny, the spirits being lethargic, and the bodyweight decreasing. Showed shortness of breath, coughing, and noisy.
Lung changes: Increased fibrotic consolidations, non-aerated lung area, and high-density lung area. Pulmonary function decreased.
Molecular changes: Increased indicators: TGF-β1, TNF-α, IL-6, and GM-CSF in bronchoalveolar lavage fluid.

Correlated Product(s): Bleomycin hydrochloride (HY-17565A)

Opposite Product(s): Neotuberostemonine (HY-N3196)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 9041-93-4
Appearance Solid
Masse moléculaire 1523 (average)
Color White to light yellow
SMILES

O=C(C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C)N[C@H](C(N[C@H](C)[C@@H](O)[C@H](C)C(N[C@]([C@H](O)C)([H])C(NCCC2=NC(C3=NC(C(N[R])=O)=CS3)=CS2)=O)=O)=O)[C@H](C4=CN=CN4)O[C@H]5[C@H]([C@H]([C@@H]([C@@H](O5)CO)O)O)O[C@]6([H])[C@H]([C@H]([C@@H]([C@H](O6)CO)O)OC(N)=O)O.NC(NCCCC[R])=N.[R]CCC[S+](C)C.O=S(O)(O)=O.[R=].[Bleomycin A2].[R=].[Bleomycin B2]
Synonyms

Bleomycin sulfate
Structure Classification
- Antibiotics
- Other Antibiotics
- Antibiotics
- Mammalian Cell Culture
- Antibiotics
- Resistance Selection
Initial Source
- Microorganisms
Streptomyces verticillus
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Publications (208)

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Cell Res

Sensing of DNA double-strand breaks by the NHEJ system stabilizes RORγt transcriptional activity and shapes Th17 pathogenicity in autoimmunity. [Abstract]

2026 Jan 7. PMID: 41495483
Drug Resist Updat

ZBP1 antagonizes MRE11-mediated DNA end resection and confers synthetic lethality to PARP inhibition in ovarian cancer. [Abstract]

2026 Jan:84:101319. PMID: 41192279
Nat Metab

The flavonoid procyanidin C1 has senotherapeutic activity and increases lifespan in mice. [Abstract]

2021 Dec;3(12):1706-1726. PMID: 34873338
Nat Cell Biol

Full-length GSDME mediates pyroptosis independent from cleavage. [Abstract]

2024 Sep;26(9):1545-1557. PMID: 38997456
Mol Cell

Lactylation of XLF promotes non-homologous end-joining repair and chemoresistance in cancer. [Abstract]

2025 Jul 17;85(14):2654-2672.e7. PMID: 40680721
ACS Nano

2026 Jun 23;20(24):17518-17535. PMID: 42246507
ACS Nano

Targeted Collagen Degradation by an MRI Probe Facilitates siRNA Delivery for Sequential Theranostics in Pulmonary Fibrosis. [Abstract]

2025 Apr 2. PMID: 40173291
Nat Commun

Age mosaic of gut epithelial cells prevents aging. [Abstract]

2025 Jul 22;16(1):6734. PMID: 40695820
Nat Commun

A Glb1-2A-mCherry reporter monitors systemic aging and predicts lifespan in middle-aged mice. [Abstract]

2022 Nov 17;13(1):7028. PMID: 36396643
J Adv Res

Plasma apolipoprotein E protein attenuates pulmonary fibrosis through LRP1 and PLAU dual receptor-mediated TGF-β/Smad inhibition. [Abstract]

2025 Dec 29:S2090-1232(25)01035-5. PMID: 41475664
J Adv Res

Elevated lactate production exacerbates PM2.5-induced pulmonary fibrosis by stabilizing TGF-β1. [Abstract]

2025 Aug 5:S2090-1232(25)00587-9. PMID: 40759348
J Adv Res

Kynurenine acts as a signaling molecule to attenuate pulmonary fibrosis by enhancing the AHR-PTEN axis. [Abstract]

2024 Jun 19:S2090-1232(24)00254-6. PMID: 38906325
Exp Mol Med

GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells. [Abstract]

2024 Dec;56(12):2739-2746. PMID: 39643607
J Biomed Sci

Doublecortin-like kinase 1 promotes fibroblast activation and fibrotic progression through Smad3 binding in idiopathic pulmonary fibrosis. [Abstract]

2026 May 22;33(1):52. PMID: 42174588
Small

Metal-Polyphenol-Network Coated Prussian Blue Nanoparticles for Synergistic Ferroptosis and Apoptosis via Triggered GPX4 Inhibition and Concurrent In Situ Bleomycin Toxification. [Abstract]

2021 Nov;17(47):e2103919. PMID: 34623753
Redox Biol

Pharmaceutical targeting of succinate dehydrogenase in fibroblasts controls bleomycin-induced lung fibrosis. [Abstract]

2021 Oct:46:102082. PMID: 34343908
J Control Release

ROS-responsive microneedles loaded with integrin avβ6-blocking antibodies for the treatment of pulmonary fibrosis. [Abstract]

2023 Aug:360:365-375. PMID: 37331606
MedComm (2020)

Matrix stiffness-induced α-tubulin acetylation is required for skin fibrosis formation through activation of Yes-associated protein. [Abstract]

2023 Jul 12;4(4):e319. PMID: 37457658
Cell Rep Med

Cross-species tropism of AAV.CPP.16 in the respiratory tract and its gene therapies against pulmonary fibrosis and viral infection. [Abstract]

2025 May 14:102144. PMID: 40409263
Cell Mol Biol Lett

Single-cell RNA sequencing identifies inherent abnormalities of adipose-derived stem cells from nonlesional sites of patients with localized scleroderma. [Abstract]

2024 Aug 30;29(1):115. PMID: 39215271
Cancer Lett

N6-methyadenosine modified SUV39H2 regulates homologous recombination through epigenetic repression of DUSP6 in gastric cancer. [Abstract]

2023 Apr 1:558:216092. PMID: 36806557
Cancer Lett

DAB2IP suppresses tumor malignancy by inhibiting GRP75-driven p53 ubiquitination in colon cancer. [Abstract]

2022 Apr 28:532:215588. PMID: 35150809
Acta Biomater

Inhaled biomimetic nanoparticles enhance pulmonary delivery of tetrandrine and pirfenidone for the treatment of idiopathic pulmonary fibrosis. [Abstract]

2025 Dec 19:S1742-7061(25)00946-8. PMID: 41423167
Adv Healthc Mater

Bioinspired Inhalable Nanoparticles Combining Chinese Herbal Tetrandrine with Pirfenidone Overcome Idiopathic Pulmonary Fibrosis via Coordinated Collagen Modulation and Fibroblast Autophagy Regulation. [Abstract]

2025 Aug 21:e01916. PMID: 40842231
Cell Death Dis

Inhibition of Rho GEFs attenuates pulmonary fibrosis through suppressing myofibroblast activation and reprogramming profibrotic macrophages. [Abstract]

2025 Apr 11;16(1):278. PMID: 40216763
Cell Death Dis

DEPTOR is a direct p53 target that suppresses cell growth and chemosensitivity. [Abstract]

2020 Nov 12;11(11):976. PMID: 33184290
Cell Death Dis

Scutellarin ameliorates pulmonary fibrosis through inhibiting NF-κB/NLRP3-mediated epithelial-mesenchymal transition and inflammation. [Abstract]

2020 Nov 13;11(11):978. PMID: 33188176

Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Nov 13;11(11):978. [Abstract]

Bleomycin (BLM) (5 mg/kg; intratracheal instillation) significantly elevated the ratio of phosphorylated p65 to total p65 (p-p65/p65) and reduced the expression level of IκBα, indicating that NF-κB signaling pathway was activated in BLM-induced pulmonary fibrosis.

Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Nov 13;11(11):978. [Abstract]

Bleomycin (BLM) (5 mg/kg; intratracheal instillation) increases the expression of NLRP3 in BALB/c mice.

Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Nov 13;11(11):978. [Abstract]

Bleomycin (BLM) (5 mg/kg; intratracheal instillation) upregulates the mRNA levels of NLRP3, ASC, IL-1β, and IL-18 in BALB/c mice.
Cell Death Dis

Oncogene PRR14 promotes breast cancer through activation of PI3K signal pathway and inhibition of CHEK2 pathway. [Abstract]

2020 Jun 15;11(6):464. PMID: 32541902
Sci China Life Sci

Local administration of liposomal-based Plekhf1 gene therapy attenuates pulmonary fibrosis by modulating macrophage polarization. [Abstract]

2023 Nov;66(11):2571-2586. PMID: 37340175

Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2023 Nov;66(11):2571-2586. [Abstract]

Bleomycin (1 mg/kg; single intratracheal injection). Results for time-course Western blot analysis of Plekhf1 and Arg1 (a macrophage M2 marker) and Col 1α (a fibrotic marker) expression in lung macrophages isolated from BLM-treated mice. Left panel: Representative Western blot images. Right panel: Bar graphs showing the data from three replicates.

Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2023 Nov;66(11):2571-2586. [Abstract]

Bleomycin (1 mg/kg; single intratracheal injection). Representative results of coimmunostaining for Plekhf1 and F4/80 in lung sections.

Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2023 Nov;66(11):2571-2586. [Abstract]

Bleomycin (1 mg/kg; single intratracheal injection). Representative results of coimmunostaining for Plekhf1 and F4/80 in BALF cells from BLM-treated mice.
Int J Biol Macromol

Role of ionizing radiation activated NRF2 in lung cancer radioresistance. [Abstract]

2023 Jun 30:241:124476. PMID: 37076059
Phytomedicine

Identification of key anti-pulmonary fibrosis components in Hyssopus cuspidatus Boriss. and its mechanism exploration. [Abstract]

2025 Dec 5:150:157676. PMID: 41389405
Phytomedicine

Network-guided discovery of Zeqi Decoction as a PPARγ-targeted therapy for pulmonary fibrosis. [Abstract]

2025 Dec 16:150:157717. PMID: 41447837
Phytomedicine

Rottlerin inhibits PKCδ to attenuate pulmonary fibrosis by suppressing NLRC4/ASC-mediated pyroptosis. [Abstract]

2025 Oct:146:157154. PMID: 40819638
Phytomedicine

Microbial signatures of areca nut chewing: Bridging the gap between oral dysbiosis and submucous fibrosis. [Abstract]

2025 Sep 4:147:157227. PMID: 40934758
Phytomedicine

Curcumin analogue EF24 prevents alveolar epithelial cell senescence to ameliorate idiopathic pulmonary fibrosis via activation of PTEN. [Abstract]

2024 Jul 27:133:155882. PMID: 39096545
Phytomedicine

Physalis Calyx seu Fructus inhibited pulmonary fibrosis through regulating Wnt/β-catenin signaling pathway. [Abstract]

2024 Jun 3:131:155797. PMID: 38878326

Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jun 3:131:155797. [Abstract]

PCF-EtOH diminished BLM (Bleomycin sulfate)-induced increase of fibronectin, vimentin and α-SMA. All mice, except those in the control group (nebulization with saline), are nebulised with BLM/saline (1 mg/mL, 8 mL) for 30 min on days 1, 4 and 7, respectively.

Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jun 3:131:155797. [Abstract]

PCF-EtOH blocked BLM (Bleomycin sulfate)-induced upregulation of β-catenin protein. All mice, except those in the control group (nebulization with saline), are nebulised with BLM/saline (1 mg/mL, 8 mL) for 30 min on days 1, 4 and 7, respectively.
Phytomedicine

Vincamine as an agonist of G protein-coupled receptor 40 effectively ameliorates pulmonary fibrosis in mice. [Abstract]

2023 Sep:118:154919. PMID: 37392673
Phytomedicine

Qimai Feiluoping decoction inhibits mitochondrial complex I-mediated oxidative stress to ameliorate bleomycin-induced pulmonary fibrosis. [Abstract]

2023 Apr:112:154707. PMID: 36805483
ACS Appl Mater Interfaces

The Clearance Effect of Tetrahedral DNA Nanostructures on Senescent Human Dermal Fibroblasts. [Abstract]

2019 Jan 16;11(2):1942-1950. PMID: 30562007
Sens Actuators B Chem

2025 Sep 4;446:138682.
J Transl Med

Vitamin D attenuates the progression of pulmonary fibrosis via inhibiting thymidine kinase 1/PFKFB3-driven glycolysis. [Abstract]

2026 Apr 22;24(1):571. PMID: 42021376
J Transl Med

Ficolin-1 ameliorates pulmonary fibrosis via directly binding to TGF-β1. [Abstract]

2024 Nov 21;22(1):1051. PMID: 39574172
J Transl Med

Deciphering the cellular and molecular landscape of pulmonary fibrosis through single-cell sequencing and machine learning. [Abstract]

2025 Jan 2;23(1):3. PMID: 39748378
J Transl Med

MicroRNA-21a-5p inhibition alleviates systemic sclerosis by targeting STAT3 signaling. [Abstract]

2024 Apr 1;22(1):323. PMID: 38561750
Biomed Pharmacother

Alpinetin ameliorates bleomycin-induced pulmonary fibrosis by repressing fibroblast differentiation and proliferation. [Abstract]

2024 Feb:171:116101. PMID: 38228032
Biomed Pharmacother

Iguratimod ameliorates bleomycin-induced pulmonary fibrosis by inhibiting the EMT process and NLRP3 inflammasome activation. [Abstract]

2022 Sep:153:113460. PMID: 36076570
Biomed Pharmacother

Dopamine receptor agonists ameliorate bleomycin-induced pulmonary fibrosis by repressing fibroblast differentiation and proliferation. [Abstract]

2021 Jul:139:111500. PMID: 33901873
Redox Rep

Prophylactic quercetin administration attenuates pulmonary fibrosis via ferroptosis-resistant priming of alveolar epithelial cells. [Abstract]

2026 Dec;31(1):2632434. PMID: 41697789
Stem Cell Res Ther

A scalable platform for EPSC-Induced MSC extracellular vesicles with therapeutic potential. [Abstract]

2025 Aug 5;16(1):426. PMID: 40765003
Stem Cell Res Ther

hAMSCs regulate EMT in the progression of experimental pulmonary fibrosis through delivering miR-181a-5p targeting TGFBR1. [Abstract]

2025 Jan 5;16(1):2. PMID: 39757225
JACC Basic Transl Sci

Ubiquitin Specific Protease 9X Regulates the Activation of ARK5 and Promotes Progression of Fibrotic Remodeling. [Abstract]

2025 Jul;10(7):101255. PMID: 40310323
Aging Cell

2026 Apr;25(4):e70462. PMID: 41910356
Cell Rep

Lactylation-driven nuclear RIG-I promoted by lactate transporter inhibitor suppresses DNA damage repair through inhibiting PARP1 activity. [Abstract]

2026 Jan 13;45(1):116854. PMID: 41538326
Cell Rep

Phosphatase PHLPP1 is an alveolar-macrophage-intrinsic transcriptional checkpoint controlling pulmonary fibrosis. [Abstract]

2025 Mar 12;44(3):115399. PMID: 40085643
Cell Rep

Mitotic ER-mitochondria contact enhances mitochondrial Ca2+ influx to promote cell division. [Abstract]

2024 Sep 28;43(10):114794. PMID: 39342616
J Med Chem

Discovery and Evaluation of a PROTAC Degrader Targeting SAMHD1 for the Treatment of Pulmonary Fibrosis. [Abstract]

2026 Apr 23;69(8):9363-9385. PMID: 41932858
J Med Chem

Identification of Nitric Oxide Donating Dasatinib Derivatives with Intraocular Pressure Lowering and Senolytic Activities. [Abstract]

2025 Apr 14. PMID: 40228166
Anal Chem

Sequential Labeling-Assisted Precise and Multitarget Analysis of Surface Proteins on Extracellular Vesicles. [Abstract]

2025 Jun 3;97(21):11073-11081. PMID: 40388194
Antioxidants (Basel)

Pitavastatin Calcium Confers Fungicidal Properties to Fluconazole by Inhibiting Ubiquinone Biosynthesis and Generating Reactive Oxygen Species. [Abstract]

2024 May 29;13(6):667. PMID: 38929106
Mol Metab

DNA damage to β cells in culture recapitulates features of senescent β cells that accumulate in type 1 diabetes. [Abstract]

2022 Aug:62:101524. PMID: 35660116
Mol Med

Nuclear VPS35 attenuates NHEJ repair by sequestering Ku protein. [Abstract]

2025 Jun 9;31(1):222. PMID: 40484931
Mol Med

LncRNA GAS5 suppresses TGF-β1-induced transformation of pulmonary pericytes into myofibroblasts by recruiting KDM5B and promoting H3K4me2/3 demethylation of the PDGFRα/β promoter. [Abstract]

2023 Mar 14;29(1):32. PMID: 36918759
J Cell Biol

DNA damage promotes microtubule dynamics through a DNA-PK-AKT axis for enhanced repair. [Abstract]

2021 Feb 1;220(2):e201911025. PMID: 33404607
Phytother Res

Bryodulcosigenin attenuates bleomycin-induced pulmonary fibrosis via inhibiting AMPK-mediated mesenchymal epithelial transition and oxidative stress. [Abstract]

2022 Oct;36(10):3911-3923. PMID: 35794782
Fundam Res

Pharmacological regulation of tissue fibrosis by targeting the mechanical contraction of myofibroblasts. [Abstract]

2021 Dec 23;2(1):37-47. PMID: 38933917
OncoImmunology

Pirfenidone facilitates immune infiltration and enhances the antitumor efficacy of PD-L1 blockade in mice. [Abstract]

2020 Sep 22;9(1):1824631. PMID: 33457101
Cell Mol Life Sci

Role of transient receptor potential ankyrin 1 in idiopathic pulmonary fibrosis: modulation of M2 macrophage polarization. [Abstract]

2024 Apr 18;81(1):187. PMID: 38635081

Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Apr 18;81(1):187. [Abstract]

Bleomycin sulfate (3 mg/kg; nebulized) significantly upregulates TRPA1 protein levels in mice.

Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Apr 18;81(1):187. [Abstract]

Bleomycin sulfate (3 mg/kg; nebulized) significantly increases TRPA1 mRNA levels in mouse lung tissues.
Ecotoxicol Environ Saf

PPARγ-responsive luciferase reporter system for high-throughput screening of chemical toxins with potential pulmonary fibrosis effects. [Abstract]

2025 Nov 15:307:119433. PMID: 41273832
Transl Res

Dach1 deficiency drives alveolar epithelium apoptosis in pulmonary fibrosis via modulating C-Jun/Bim activity. [Abstract]

2023 Jul:257:54-65. PMID: 36754276
J Invest Dermatol

Excess KLHL24 impairs skin wound healing by degradation of vimentin. [Abstract]

2023 Jul;143(7):1289-1298.e15. PMID: 36716923
Chin Med

TMT proteomics analysis reveals the mechanism of bleomycin-induced pulmonary fibrosis and effects of Ginseng honeysuckle superfine powdered tea. [Abstract]

2023 May 24;18(1):60. PMID: 37221600
Biochem Pharmacol

Targeting PBK in the fibroblast-to-myofibroblast transition: A novel therapeutic strategy for IPF. [Abstract]

2025 Aug 28;242(Pt 2):117283. PMID: 40885317
Biochem Pharmacol

NLRP3 inflammasome activation contributes to the development of the pro-fibrotic phenotype of lung fibroblasts. [Abstract]

2024 Aug 17:116496. PMID: 39159876
Biochem Pharmacol

Nrf2 in keratinocytes protects against skin fibrosis via regulating epidermal lesion and inflammatory response. [Abstract]

2020 Apr;174:113846. PMID: 32032580
Drug Deliv Transl Res

Propellant-free intrawound antibiotic foam for intraoperative antimicrobial prophylaxis. [Abstract]

2026 Jul;16(7):2514-2526. PMID: 42050315
J Ethnopharmacol

Study on the anti-pulmonary fibrosis mechanism of Renshen Pingfei formula by inhibiting M2 macrophage polarization and regulating TGF - β/SMAD signaling pathway. [Abstract]

2026 May 23:363:121465. PMID: 41780616
J Ethnopharmacol

A novel polysaccharide from Qamgur (Brassica rapa L.) mitigates pulmonary fibrosis by regulating inflammatory, epithelial-mesenchymal transition, and fibrotic pathways. [Abstract]

2026 Apr 6:360:121174. PMID: 41500353
J Ethnopharmacol

Mimosa pudica L. extract ameliorates pulmonary fibrosis via modulation of MAPK signaling pathways and FOXO3 stabilization. [Abstract]

2024 Aug 10:330:118226. PMID: 38670401
Am J Respir Cell Mol Biol

DOCK2 Promotes Pleural Fibrosis by Modulating Mesothelial to Mesenchymal Transition. [Abstract]

2022 Feb;66(2):171-182. PMID: 34710342
Am J Respir Cell Mol Biol

Clioquinol Attenuates Pulmonary Fibrosis through Inactivation of Fibroblasts via Iron Chelation. [Abstract]

2021 Aug;65(2):189-200. PMID: 33861690
Am J Respir Cell Mol Biol

Kindlin-2 Acts as a Key Mediator of Lung Fibroblast Activation and Pulmonary Fibrosis Progression. [Abstract]

2021 Jul;65(1):54-69. PMID: 33761308
Cells

ML216 Prevents DNA Damage-Induced Senescence by Modulating DBC1-BLM Interaction. [Abstract]

2022 Dec 29;12(1):145. PMID: 36611939
Cells

Beyond the Warburg Effect: Oxidative and Glycolytic Phenotypes Coexist within the Metabolic Heterogeneity of Glioblastoma. [Abstract]

2021 Jan 20;10(2):202. PMID: 33498369
Drug Des Devel Ther

2026 Apr 7:20:592770. PMID: 41970211
Commun Biol

Chloride intracellular channel CLIC3 mediates fibroblast cellular senescence by interacting with ERK7. [Abstract]

2025 Jan 14;8(1):51. PMID: 39809890
Mech Ageing Dev

Calcium transferring from ER to mitochondria via miR-129/ITPR2 axis controls cellular senescence in vitro and in vivo. [Abstract]

2024 Apr:218:111902. PMID: 38218462

Bleomycin sulfate purchased from MedChemExpress. Usage Cited in: Mech Ageing Dev. 2024 Apr:218:111902. [Abstract]

miR-129 delays bleomycin-induced cellular senescence. MRC5 cells were transfected with miR-Ctrl or miR-129 and then treated with bleomycin (1 µg/ml) where indicated. Four days after transfection, the p-H2AX, ITPR2, p16, p21 and GAPDH protein levels were quantified by western blot.
Life Sci

Autophagy-related protein EI24 delays the development of pulmonary fibrosis by promoting autophagy. [Abstract]

2021 Jan 1;264:118664. PMID: 33127511
Respir Res

Dehydrocorydaline attenuates bleomycin-induced pulmonary fibrosis by inhibiting fibroblast activation. [Abstract]

2025 Apr 12;26(1):136. PMID: 40221718
Respir Res

MiR-125b-5p alleviates pulmonary fibrosis by inhibiting TGFβ1-mediated epithelial-mesenchymal transition via targeting BAK1. [Abstract]

2024 Oct 19;25(1):382. PMID: 39427175
Inflammation

Toll-Like Receptor 9 Aggravates Pulmonary Fibrosis by Promoting NLRP3-Mediated Pyroptosis of Alveolar Epithelial Cells. [Abstract]

2024 Oct;47(5):1744-1761. PMID: 38498270
Inflammation

Nintedanib Ameliorates Bleomycin-Induced Pulmonary Fibrosis, Inflammation, Apoptosis, and Oxidative Stress by Modulating PI3K/Akt/mTOR Pathway in Mice. [Abstract]

2023 Aug;46(4):1531-1542. PMID: 37160579
Respir Res

Long non-coding RNA H19 deficiency ameliorates bleomycin-induced pulmonary inflammation and fibrosis. [Abstract]

2020 Nov 2;21(1):290. PMID: 33138822
Respir Res

Curdione ameliorates bleomycin-induced pulmonary fibrosis by repressing TGF-β-induced fibroblast to myofibroblast differentiation. [Abstract]

2020 Feb 19;21(1):58. PMID: 32075634
Int J Mol Sci

Integrating Network Pharmacology, Machine Learning, and Experimental Validation to Elucidate the Mechanism of Cardamonin in Treating Idiopathic Pulmonary Fibrosis. [Abstract]

2025 Dec 25;27(1):249. PMID: 41516127
Biomolecules

Notch Signaling Exacerbates Pulmonary Fibrosis by Regulating the Differentiation of CD4+ Tissue-Resident Memory T Cells. [Abstract]

2026 Feb 20;16(2):328. PMID: 41750395
Pharmaceuticals (Basel)

Comparison of the Results of Modeling Pulmonary Fibrosis in Sprague Dawley Rats by Intratracheal Administration of Bleomycin in the Form of Sulfate and Chloride at a Dose of 3 mg/kg. [Abstract]

2024 Oct 11;17(10):1360. PMID: 39459000
Front Pharmacol

TRAF6 Suppresses the Development of Pulmonary Fibrosis by Attenuating the Activation of Fibroblasts. [Abstract]

2022 May 20;13:911945. PMID: 35668944
Eur J Pharmacol

Miltefosine attenuates silicosis involving inhibiting the Akt/mTOR signaling pathway. [Abstract]

2026 May 27:178931. PMID: 42203065
Int Immunopharmacol

BI-2536 attenuates IPF progression by inhibiting the PLK2/JNK/SP1 Signaling pathway in AT2 cells. [Abstract]

2026 Jan 1;168(Pt 1):115812. PMID: 41237696
Int Immunopharmacol

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2023 Jul:120:110369. PMID: 37224650
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2023 Mar:116:109723. PMID: 36696855
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2022 Dec;113(Pt A):109409. PMID: 36461602
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Biol Proced Online

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2025 Jun 16;27(1):21. PMID: 40524168
J Cell Mol Med

2026 May;30(10):e71168. PMID: 42179136
Biochim Biophys Acta Mol Basis Dis

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2026 Feb;1872(2):168100. PMID: 41192648
FASEB J

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2025 Oct 15;39(19):e71083. PMID: 41001804
Lab Invest

Senescent Epithelial Cells Serve as Invasive Growth Drivers in Ameloblastoma. [Abstract]

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2025 Mar 12;1871(5):167798. PMID: 40081619
FEBS J

TRPM7 controls skin keratinocyte senescence by targeting intracellular calcium signaling. [Abstract]

2024 Nov;291(21):4680-4695. PMID: 39185948
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2022 Mar 3:2022:6755407. PMID: 35281428
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iScience

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2025 Oct 22.
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2026 Jan 8;16(1):4864. PMID: 41507413
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2025 May 9:2025:1416567. PMID: 40386129
Pathol Res Pract

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Zerumbone alleviated bleomycin-induced pulmonary fibrosis in mice via SIRT1/Nrf2 pathway. [Abstract]

2024 Nov;397(11):8979-8992. PMID: 38874804
Front Biosci (Landmark Ed)

CD5L Deficiency Protects Mice Against Bleomycin-Induced Pulmonary Fibrosis. [Abstract]

2023 Sep 22;28(9):209. PMID: 37796694
Bioorg Med Chem

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Immunopharmacol Immunotoxicol

Atractylenolide III attenuates bleomycin-induced experimental pulmonary fibrosis and oxidative stress in rat model via Nrf2/NQO1/HO-1 pathway activation. [Abstract]

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Toxicol Lett

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Ther Adv Chronic Dis

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FEMS Yeast Res

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BMC Pharmacol Toxicol

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Fitoterapia

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Mutat Res Genet Toxicol Environ Mutagen

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Saroglitazar Ameliorates Pulmonary Fibrosis Progression in Mice by Suppressing NF-κB Activation and Attenuating Macrophage M1 Polarization. [Abstract]

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Immunobiology

HMGB1 regulates the activation of dendritic cells and CD4+ T cell responses through the modulation of autophagy in bleomycin-induced pulmonary fibrosis. [Abstract]

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Exp Ther Med

MicroRNA‑203a‑3p improves bleomycin and pingyangmycin sensitivity by inactivating the PI3K/AKT pathway in hemangioma. [Abstract]

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Macrophage erythropoietin receptor signaling drives macrophage-myofibroblast transition via JAK2/STAT3 and TGF-β1/Smad3 pathways to exacerbate pulmonary fibrosis in mice. [Abstract]

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Porcine respiratory disease complex induces pulmonary fibrosis related to the aberrant sphingolipid metabolism. [Abstract]

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The hematopoietic activity of EPO is unfavorable to the treatment of bleomycin-induced pulmonary fibrosis in mice. [Abstract]

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2025 May 4.
Nat Prod Commun

2024 Mar 29.
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2024 Jun 28.
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2023 Jul 22, 101823.
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2026 May 16.
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2026 Apr 14.
bioRxiv

2026 Apr 30.
SSRN

2025 Dec 12.
medRxiv

2025 Nov 28.
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bioRxiv

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2025 May 26.
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bioRxiv

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bioRxiv

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bioRxiv

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bioRxiv

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2024 Oct 31.
MedComm Futur Med

2024 Jul 19.
bioRxiv

2024 July 27.
Research Square Preprint

2023 Nov 23.
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Research Square Preprint

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Research Square Preprint

2023 Aug 4.
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Universidad de las Illes Balears

Facultad de Ciencias, 2022 Feb.
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23 December 2021.
Oxid Med Cell Longev

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2021 Nov 1:2021:6241242. PMID: 34760046
Oxid Med Cell Longev

Gasotransmitter CO Attenuates Bleomycin-Induced Fibroblast Senescence via Induction of Stress Granule Formation. [Abstract]

2021 Jun 29:2021:9926284. PMID: 34306316
Patent

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Patent

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Solvant et solubilité

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Références

[1]. Hovhannisyan G, et al. Comparative analysis of individual chromosome involvement in micronuclei induced by bleomycin in human leukocytes. Mol Cytogenet. 2016 Jun 21;9:49. [Content Brief]

[2]. Jaaskela-Saari HA, et al. Squamous cell cancer cell lines: sensitivity to bleomycin and suitability for animal xenograft studies. Acta Otolaryngol Suppl. 1997;529:241-4. [Content Brief]

[3]. Corboz MR, et al. Therapeutic administration of inhaled INS1009, a treprostinil prodrug formulation, inhibits bleomycin-induced pulmonary fibrosis in rats. Pulm Pharmacol Ther. 2018 Apr;49:95-103. [Content Brief]

[4]. Ling Peng, et al. Scutellarin ameliorates pulmonary fibrosis through inhibiting NF-κB/NLRP3-mediated epithelial-mesenchymal transition and inflammation. Cell Death Dis. 2020 Nov 13;11(11):978. [Content Brief]

[5]. Kang Miao, et al. Scutellarein inhibits BLM-mediated pulmonary fibrosis by affecting fibroblast differentiation, proliferation, and apoptosis. Ther Adv Chronic Dis. 2020 Jul 30;11:2040622320940185. [Content Brief]

[6]. Brandt JP, et, al. Bleomycin. National Library of Medicine. August 28, 202

[7]. Gul A, et, al. Pulmonary fibrosis model of mice induced by different administration methods of bleomycin. BMC Pulm Med. 2023 Mar 21;23(1):91. [Content Brief]

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Bleomycin sulfate

208 Cited Publications

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