Methyl syringate
Based on 1 Customer Validation
Methyl syringate is a selective TRPA1 agonist. Methyl syringate regulates food intake and gastric emptying through a TRPA1-mediated pathway. Methyl syringate is an efficient phenolic mediator for bacterial and fungal laccases. Methyl syringate is a chemical marker of Asphodel monofloral honey. Methyl syringate contributes to the antibacterial activity of honey. Methyl syringate inhibits aflatoxin production. Methyl syringate can contribute to weight suppression. Methyl syringate can be studies for cancer prevention (e.g. lung cancer), suppression of hypoxia-induced inflammatory response and tumorigenesis.
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- Pureté: 99.96%
- CAS No.: 884-35-5
- Formule: C10H12O5
- Masse moléculaire:212.20
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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HY-W002116R
Methyl syringate (Standard)
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HY-108449
Acoltremon
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HY-Y0189
Methyl Salicylate
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HY-W014449
4-Nitrophenyl butyrate
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HY-Y0189R
Methyl Salicylate (Standard)
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HY-N2908
Atraric acid
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HY-W012399
2,5-Di-tert-butylhydroquinone
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HY-W002314
2-Bromo-4'-hydroxyacetophenone
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HY-W002751
6-Methylsalicylic acid
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HY-W016491
Isopropyl 4-hydroxybenzoate
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HY-N3287
Methyl 3-hydroxy-4,5-dimethoxybenzoate
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HY-34580
Methyl 3,5-dihydroxybenzoate
Activité biologique
TRPA1[3].
Methyl syringate (0.1-1 mM, pretreatment for 1 h then co-treatment under hypoxia for 24 h) effectively blocks hypoxia-induced COX-2 protein expression is a dose dependent manner and inhibits induction of COX-2 mRNA and promoter activity in A549 cells under hypoxic conditions[4].
Methyl syringate (1 mM, 1 h) represses COX-2 and cell migration in HT-1080 cells[4].
Methyl syringate (1-100 μM) inhibits catalytic activity of PTPN2 and PTPN6 with IC50 values of 6.95 and 7.31 μM in E. coli cells overexpressing PTPN2 and PTPN6[5].
Methyl syringate (10-20 μM, 6 h) stimulates the glucose uptake in differentiated 3T3-L1 adipocytes and C2C12 muscle cells[5].
Methyl syringate (5-20 μM, 6 h) significantly increases AMPK phosphorylation in 3T3-L1 adipocytes at 10 or 20 μM dosage[5].
Methyl syringate (1-20 μM, 6 d) does not affect adipocyte differentiation in differentiated 3T3-L1 preadipocytes, indicating it does not induce lipid accumulation[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549 under hypoxic conditions
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Concentration:1 mM
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Incubation Time:1 h
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Result:Inhibited hypoxia-induced COX-2 protein through TRPA1.
Was not affected by Sirt1 enzyme on its inhibition of hypoxia-induced COX-2 protein expression. Was blocked by HC-030031 (HY-15064) (100 μM) on its inhibition of hypoxia-induced COX-2 protein expression under hypoxic conditions.
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Cell Line:A549 under hypoxic conditions
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Concentration:1 mM
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Incubation Time:1 h
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Result:Decreased A549 migration under hypoxic conditions by approximately 40%.
Blocked by HC-030031 (100 μM) on its inhibition of cell migration, suggesting that its effects on hypoxia-induced migration are mediated by TRPA1.
Decreased hypoxia-enhanced A549 invasion.
Methyl syringate (0.1-10 mg/kg, p.o., one single dose) significantly decreases the food remaining in the stomach 28 h after treatment in male ICR mice [1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male ICR mice experienced fasting and allowed ad libitum access of food and water[1]
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Dosage:0.1-10 mg/kg
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Administration:Oral gavage (p.o.)
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Result:Did not alter the body weight significantly compared to the control.
Delayed gastric emptying dose-dependently with around 70% reduction at 10 mg/kg compared to the control.
Increased plasma PYY levels 15 min after treatment but did not affect GLP-1 levels.
Chemical Information
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CAS No. 884-35-5
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Appearance Solid
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Masse moléculaire 212.20
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Formule C10H12O5
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Color White to off-white
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SMILES
O=C(OC)C1=CC(OC)=C(O)C(OC)=C1
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvant et solubilité
DMSO : 100 mg/mL (471.25 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.78 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.78 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Kim MJ, et al. The TRPA1 agonist, methyl syringate suppresses food intake and gastric emptying. PLoS One. 2013 Aug 21;8(8):e71603. [Content Brief]
[2]. Rosado T, et al. Methyl syringate: an efficient phenolic mediator for bacterial and fungal laccases. Bioresour Technol. 2012 Nov;124:371-8. [Content Brief]
[5]. Tuberoso CI, et al. Methyl syringate: a chemical marker of asphodel (Asphodelus microcarpus Salzm. et Viv.) monofloral honey. J Agric Food Chem. 2009 May 13;57(9):3895-900. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.7125 mL | 23.5627 mL | 47.1254 mL | 117.8134 mL |
| 5 mM | 0.9425 mL | 4.7125 mL | 9.4251 mL | 23.5627 mL | |
| 10 mM | 0.4713 mL | 2.3563 mL | 4.7125 mL | 11.7813 mL | |
| 15 mM | 0.3142 mL | 1.5708 mL | 3.1417 mL | 7.8542 mL | |
| 20 mM | 0.2356 mL | 1.1781 mL | 2.3563 mL | 5.8907 mL | |
| 25 mM | 0.1885 mL | 0.9425 mL | 1.8850 mL | 4.7125 mL | |
| 30 mM | 0.1571 mL | 0.7854 mL | 1.5708 mL | 3.9271 mL | |
| 40 mM | 0.1178 mL | 0.5891 mL | 1.1781 mL | 2.9453 mL | |
| 50 mM | 0.0943 mL | 0.4713 mL | 0.9425 mL | 2.3563 mL | |
| 60 mM | 0.0785 mL | 0.3927 mL | 0.7854 mL | 1.9636 mL | |
| 80 mM | 0.0589 mL | 0.2945 mL | 0.5891 mL | 1.4727 mL | |
| 100 mM | 0.0471 mL | 0.2356 mL | 0.4713 mL | 1.1781 mL |
Methyl syringate Related Classifications
HY-W002116 Related Classifications
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