Cyclopiazonic acid

Name: Cyclopiazonic acid
Brand: MedChemExpress
SKU: HY-N6771
Rating: 5.0 (7 reviews)

Cat. No.: HY-N6771 Purity: 99.86%: Data Sheet
COA

SDS
Handling Instructions
FAQs
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Cyclopiazonic acid is an endoplasmic reticulum calcium ATPase (ECAs) inhibitor and human respiratory syncytial virus (RSV) inhibitor (EC₅₀ value of 4.13 μ M), which can reduce the antagonistic effect of 5-HT receptors in rat thoracic aorta, induce p53 dependent cell apoptosis and reproductive toxicity in mouse testes, and inhibit the biological activation of aflatoxin B^[1][4][5].

For research use only. We do not sell to patients.

CAS No. : 18172-33-3

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Cyclopiazonic acid:

Cyclopiazonic acid-¹³C₂₀ Get quote
Cyclopiazonic acid (Standard) Get quote

7 Publications Citing Use of MCE Cyclopiazonic acid

•Mol Biol Rep. 2025 Feb 12;52(1):223. [Abstract]

Others

Cell Proliferation/Viability Assay

: Cyclopiazonic acid purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Dec 1;17(1):253. [Abstract]; Neuronal MMP decrease induced by Glu exposure was abolished by Ca2+-free buffer, suppressed by preincubation with BAPTA-AM (10 μM; 30 min) but not CPA (Cyclopiazonic acid: 10 μM; 10 min).

: Cyclopiazonic acid purchased from MedChemExpress. Usage Cited in: Mol Biol Rep. 2025 Feb 12;52(1):223. [Abstract]; Protein expression of STIM1 and ORAI1 in melanocytes pretreated with CPA (Cyclopiazonic acid: 2.5 µM).

: Cyclopiazonic acid purchased from MedChemExpress. Usage Cited in: Mol Biol Rep. 2025 Feb 12;52(1):223. [Abstract]; Cell viability of STIM1 and ORAI1 in melanocytes pretreated with CPA (Cyclopiazonic acid: 2.5 µM).

: Cyclopiazonic acid purchased from MedChemExpress. Usage Cited in: Genes Dis. 2024 Mar 28;11(6):101281.; Calcium imaging was applied to analyze the calcium mobilization in Con- and SR-Hep3B and MHCC97H cells, respectively upon cyclopiazonic acid (CPA) (200 μM) stimulation.

: Cyclopiazonic acid purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2022 Apr 15;132(8):e149160. [Abstract]; CPA (Cyclopiazonic acid: 5 μM) inhibited SERCA and promoted Ca2+ efflux from ER.

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N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

View All 5-HT Receptor Isoform Specific Products:

View All Isoforms

5-HT Receptor 5-HT₁ Receptor 5-HT₂ Receptor 5-HT₃ Receptor 5-HT₄ Receptor 5-HT₅ Receptor 5-HT₆ Receptor 5-HT₇ Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
Sf9	IC₅₀	0.01 μM Compound: 1, CPA	Inhibition of Heliothis virescens SERCA expressed in Sf9 cells assessed as ATP depletion measured after 60 mins by luciferin/luciferase coupled reporter gene assay Inhibition of Heliothis virescens SERCA expressed in Sf9 cells assessed as ATP depletion measured after 60 mins by luciferin/luciferase coupled reporter gene assay	[PMID: 21719297]

In Vitro

Cyclopiazonic acid (10 μM) enhances 5-HT-induced contraction and reduces the antagonistic effect of 5-HT receptors in rat thoracic aorta ^[2].
Cyclopiazonic acid (10 μM, 48h) has an inhibitory effect on pacemaker current in sinoatrial node cells^[3].
Cyclopiazonic acid (10 μM, 48h) exhibits antiviral activity against human respiratory syncytial virus by increasing intracellular calcium concentration in HEp-2 cells^[4].
Cyclopiazonic acid (10 μM) inhibits the formation of certain metabolites of caffeine and testosterone and suppresses the biological activation of aflatoxin B through cytochrome P450 enzymes^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3].

Cell Line:	Isolated sinoatrial node (SAN) cells
Concentration:	10 μM
Incubation Time:	48 h
Result:	Reduced the expression of HCN4 protein.

In Vivo

Cyclopiazonic acid (0.03, 0.06, 0.12 mg/kg; once a day; 28 days; i.p.) induces p53 dependent cell apoptosis and reproductive toxicity in mouse testes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Adult male mice^[1].
Dosage:	0.03, 0.06, 0.12 mg/kg
Administration:	Intraperitoneal injection (i.p.); once a day; 28 days
Result:	Increased the levels of malondialdehyde (MDA), NO, and CO in testicular tissue, and reduced the concentration of total thiol molecules (TTM) and the level of total antioxidant capacity (TAC).

Molecular Weight

336.38

Formula

C₂₀H₂₀N₂O₃

CAS No.

18172-33-3

Appearance

Solid

Color

Off-white to yellow

SMILES

O=C1C(C(C)=O)=C(O)[C@@]([C@]23[H])([H])N1C(C)(C)[C@]2([H])CC4=CC=CC5=C4C3=CN5

Structure Classification

Initial Source

Microorganisms

Aspergillus flavus

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (297.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9728 mL	14.8641 mL	29.7283 mL
5 mM	0.5946 mL	2.9728 mL	5.9457 mL
10 mM	0.2973 mL	1.4864 mL	2.9728 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.86%

Select Batch:

Data Sheet (284 KB) SDS (479 KB)

COA (258 KB) Elemental Analysis Report (116 KB)

Handling Instructions (2659 KB)

References

[1]. Bonyadi F, et al. Cyclopiazonic acid induced p53-dependent apoptosis in the testis of mice: Another male related risk factor of infertility. Environ Toxicol. 2021 May;36(5):903-913. [Content Brief]

[2]. Selli C, et al. Cyclopiazonic acid alters serotonin-induced responses in rat thoracic aorta. Vascul Pharmacol. 2014 May-Jun;61(2-3):43-8. [Content Brief]

[3]. Chen Q, et al. Inhibitory Effects of Cyclopiazonic Acid on the Pacemaker Current in Sinoatrial Nodal Cells. Neuroscience. 2020 May 1;433:230-240. [Content Brief]

[4]. Cui R, et al. Cyclopiazonic acid, an inhibitor of calcium-dependent ATPases with antiviral activity against human respiratory syncytial virus. Antiviral Res. 2016 Aug;132:38-45. [Content Brief]

[5]. Kuilman-Wahls ME, et al. Cyclopiazonic acid inhibits mutagenic action of aflatoxin B(1). Environ Toxicol Pharmacol. 2002 Jul;11(3-4):207-12. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9728 mL	14.8641 mL	29.7283 mL	74.3207 mL
	5 mM	0.5946 mL	2.9728 mL	5.9457 mL	14.8641 mL
	10 mM	0.2973 mL	1.4864 mL	2.9728 mL	7.4321 mL
	15 mM	0.1982 mL	0.9909 mL	1.9819 mL	4.9547 mL
	20 mM	0.1486 mL	0.7432 mL	1.4864 mL	3.7160 mL
	25 mM	0.1189 mL	0.5946 mL	1.1891 mL	2.9728 mL
	30 mM	0.0991 mL	0.4955 mL	0.9909 mL	2.4774 mL
	40 mM	0.0743 mL	0.3716 mL	0.7432 mL	1.8580 mL
	50 mM	0.0595 mL	0.2973 mL	0.5946 mL	1.4864 mL
	60 mM	0.0495 mL	0.2477 mL	0.4955 mL	1.2387 mL
	80 mM	0.0372 mL	0.1858 mL	0.3716 mL	0.9290 mL
	100 mM	0.0297 mL	0.1486 mL	0.2973 mL	0.7432 mL