Dacarbazine citrate
Based on 23 publication(s) in Google Scholar
Dacarbazine citrate is a cell cycle nonspecific antineoplastic alkylating agent. Dacarbazine citrate inhibits T and B lymphoblastic response, with IC50 values of 50 and 10 μg/mL, respectively. Dacarbazine Citrate can be used for the research of apoptosis and various cancers such as metastatic malignant melanoma.
For research use only. We do not sell to patients.
- CAS No.: 64038-56-8
- Formula: C12H18N6O8
- Molecular Weight:374.31
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) Dacarbazine citrate
More- Nature. 2025 Jul;643(8070):192-200. [Abstract]
- Nature. 2023 Jun;618(7964):374-382. [Abstract]
- Mol Cancer. 2025 Oct 2;24(1):238. [Abstract]
- Nat Commun. 2024 Sep 10;15(1):7923. [Abstract]
- Adv Sci (Weinh). 2024 Dec;11(48):e2408707. [Abstract]
- Theranostics. 2020 Jul 25;10(21):9477-9494. [Abstract]
- J Nanobiotechnology. 2023 Oct 19;21(1):383. [Abstract]
- Carbohydr Polym. 2024 Dec 15:346:122645. [Abstract]
- Cell Rep Med. 2026 Feb 17;7(2):102621. [Abstract]
- Cell Rep Med. 2025 Apr 2:102053. [Abstract]
- Food Chem. 2025 May 31:489:144992. [Abstract]
- Food Chem. 2023 Mar 30;405(Pt A):134807. [Abstract]
- Cell Death Dis. 2025 Apr 9;16(1):268. [Abstract]
- Food Res Int. 2026 Feb 6.
- Phytother Res. 2024 Jun;38(6):2800-2817. [Abstract]
- J Ethnopharmacol. 2024 Apr 24:324:117759. [Abstract]
- J Mol Med (Berl). 2019 Aug;97(8):1183-1193. [Abstract]
- Heliyon. 2024 Jan 18;10(3):e24988. [Abstract]
- Exp Cell Res. 2020 Aug 1;393(1):112054. [Abstract]
- J Cancer Res Clin Oncol. 2025 Dec 17;152(1):12. [Abstract]
- J Sep Sci. 2025 Nov;48(11):e70312. [Abstract]
- New J Chem. 03 Aug 2022.
- SSRN. 2024 Mar 20.
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Biological Activity
Dacarbazine citrate has less pronounced inhibition of mitogenesis with IC50 values of 50 and 10 μg/mL for T and B cells, respectively[2].
Dacarbazine citrate (30 μM, 0-14 min) evokes a concentration-dependent calcium response in hTRPA1-HEK293 cells with an EC50 value of 23 μM and selectively activates the human TRPA1 channel[3].
Dacarbazine citrate (100-10,000 μM, 24 h) has cytotoxic action on B16-F10 melanoma cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:B16-F10 cell
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Concentration:100-10,000 μM
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Incubation Time:24 h
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Result:Reduced cell viability to 3,000 and 10,000 uM, with an inhibition percentage of 62 ± 2% after 24 h of incubation.
Dacarbazine citrate (1 mg/kg, i.p.; 1, 3, 5 and 7 days for chronic pain or 1 mg/kg, i.p. for acute treatment) induces mechanical and cold allodynia in mice[3].
Dacarbazine citrate-induced nociception can be reduced by TRPA1-deficient mice and antisense oligonucleotide for the TRPA1 receptor[3].
Dacarbazine citrate-induced chronic nociception can be reduced by selective TRPA1 receptor antagonists and antioxidants[3].
Dacarbazine citrate-induced nociception can be resisted by RPA1 antagonist or an antioxidant in a tumor-associated cancer pain model[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6, Trpa1+/+ or Trpa1−/− mice[3]
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Dosage:1 mg/kg
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Administration:1 mg/kg, i.p. (for acute treatment); 1 mg/kg, i.p.; 1, 3, 5 and 7 days (for chronic pain)
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Result:Caused mechanical allodynia with acute or repeated administration.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 64038-56-8
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Molecular Weight 374.31
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Formula C12H18N6O8
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SMILES
O=C(C1=C(/N=N/N(C)C)NC=N1)N.O=C(CC(C(O)=O)(O)CC(O)=O)O
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Synonyms
Imidazole Carboxamide citrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (23)
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Journal Impact Factor
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Most Recent
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Nature
2025 Jul;643(8070):192-200. PMID: 39695227 -
Nature
2023 Jun;618(7964):374-382. PMID: 37225988
Dacarbazine citrate purchased from MedChemExpress. Usage Cited in: Nature. 2023 Jun;618(7964):374-382. [Abstract]
Dacarbazine (60 mg/kg; i.p.; every other day for 8 d) significantly reduced red blood cell (RBC), reticulocyte (RET), and hemoglobin (HGB) counts in PBS- or B16F10-CL-EVP-educated mice.
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Mol Cancer
Hsa_circ_0038737 promotes PARPi resistance in castration-resistant prostate cancer via IGF2BP3-mediated DNPH1 mRNA stabilization. [Abstract]2025 Oct 2;24(1):238. PMID: 41039575 -
Nat Commun
2024 Sep 10;15(1):7923. PMID: 39256387 -
Adv Sci (Weinh)
Patient-Derived Melanoma Immune-Tumoroids as a Platform for Precise High throughput Drug Screening. [Abstract]2024 Dec;11(48):e2408707. PMID: 39475010
Dacarbazine citrate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Dec;11(48):e2408707. [Abstract]
Dacarbazine (DTIC) (500 µM; 72 h) reduced the diameter and metabolic activity of MCTs except monoculture tumoroids, especially the triples and quadruples, resulting in a decrease of approximately 32% and 28% in relative diameter, respectively, and caused a high number of apoptotic cells to detach from the main structure.
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Theranostics
Molecular signatures of BRCAness analysis identifies PARP inhibitor Niraparib as a novel targeted therapeutic strategy for soft tissue Sarcomas. [Abstract]2020 Jul 25;10(21):9477-9494. PMID: 32863940 -
J Nanobiotechnology
Combination of ferroptosis and pyroptosis dual induction by triptolide nano-MOFs for immunotherapy of Melanoma. [Abstract]2023 Oct 19;21(1):383. PMID: 37858186
Dacarbazine citrate purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Oct 19;21(1):383. [Abstract]
Dacarbazine (DAC) (5 mg/kg; i.v.; once every three days for 3 times) significantly inhibited the growth of subcutaneous B16F10 melanoma xenografts in C57BL/6 mice.
Dacarbazine citrate purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Oct 19;21(1):383. [Abstract]
H&E staining of tumors in each group at the end of treatment. The results showed that Dacarbazine (DAC) (5 mg/kg; i.v.; once every three days for 3 times) caused significant cell necrosis and nucleopaenia in tumors of B16F10 tumor-bearing mice. Scale bar, 100 μm.
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Carbohydr Polym
Chitosan/dextran-based organohydrogel delivers EZH2 inhibitor to epigenetically reprogram chemo/immuno-resistance in unresectable metastatic melanoma. [Abstract]2024 Dec 15:346:122645. PMID: 39245506 -
Cell Rep Med
Bacterial vesicles from intratumoral L. salivarius enhance PD-1 blockade via FPR1-mediated macrophage polarization in gastric cancer. [Abstract]2026 Feb 17;7(2):102621. PMID: 41707647 -
Cell Rep Med
CAN-Scan: A multi-omic phenotype-driven precision oncology platform identifies prognostic biomarkers of therapy response for colorectal cancer. [Abstract]2025 Apr 2:102053. PMID: 40187357 -
Food Chem
Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]2025 May 31:489:144992. PMID: 40466530 -
Food Chem
Discovery of novel ascorbic acid derivatives and other metabolites in fruit of Rosa roxburghii Tratt through untargeted metabolomics and feature-based molecular networking. [Abstract]2023 Mar 30;405(Pt A):134807. PMID: 36370576 -
Cell Death Dis
MTCH2 regulates NRF2-mediated RRM1 expression to promote melanoma proliferation and dacarbazine insensitivity. [Abstract]2025 Apr 9;16(1):268. PMID: 40204724
Dacarbazine citrate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Apr 9;16(1):268. [Abstract]
Dacarbazine (20 mg/kg; i.p.; 20 d) reduced tumor growth rates and tumor sizes of melanoma xenograft mice.
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Phytother Res
Isoalantolactone exerts anti-melanoma effects via inhibiting PI3K/AKT/mTOR and STAT3 signaling in cell and mouse models. [Abstract]2024 Jun;38(6):2800-2817. PMID: 38526171 -
J Ethnopharmacol
Screening of anti-melanoma compounds from Morus alba L.: Sanggenon C promotes melanoma cell apoptosis by disrupting intracellular Ca2+ homeostasis. [Abstract]2024 Apr 24:324:117759. PMID: 38219884 -
J Mol Med (Berl)
2019 Aug;97(8):1183-1193. PMID: 31201471 -
Heliyon
2024 Jan 18;10(3):e24988. PMID: 38317912 -
Exp Cell Res
Network-based analysis with primary cells reveals drug response landscape of acute myeloid leukemia. [Abstract]2020 Aug 1;393(1):112054. PMID: 32376287 -
J Cancer Res Clin Oncol
Comprehensive analysis based on spatial domains identifies CD44 as a potential target of puerarin. [Abstract]2025 Dec 17;152(1):12. PMID: 41405724 -
J Sep Sci
Cell Metabolomics Reveals the Hepatotoxic Mechanism of Copper in Normal Rat Liver Cells Using Reversed-Phase and Hydrophilic Interaction Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry. [Abstract]2025 Nov;48(11):e70312. PMID: 41172059 -
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Purity & Documentation
References
[1]. Abdullah A Al-Badr, et al. Dacarbazine. Profiles Drug Subst Excip Relat Methodol [Content Brief]
[2]. J M Rojo, et al. Inhibition of T and B lymphoblastic response by mithramycin, dacarbazine, prospidium chloride and peptichemio. Chemotherapy. 1983;29(5):345-51. [Content Brief]
[3]. Int J Cancer, et al. Dacarbazine alone or associated with melanoma-bearing cancer pain model induces painful hypersensitivity by TRPA1 activation in mice. Int J Cancer. 2020 May 15;146(10):2797-2809. [Content Brief]
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