Dentonin TFA
Dentonin TFA (AC-100 TFA) is a synthetic fragment derived from MEPE. Dentonin TFA enhances osteogenesis by promoting osteoprogenitor adhesion and facilitates immature adherent cells survival. Dentonin TFA has no significant effect to mature osteoblasts. Dentonin TFA can be used for the research of phosphate homeostasis and bone metabolism.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Formel: C109H161F3N30O44
- Molecular Weight:2652.61
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Chemical Information
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- Reinheit & Dokumentation
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
MEPE is a a member of the SIBLING (Small Integrin-Binding Ligand, N-linked Glycoprotein) family of secreted glycophosphoproteins.? MEPE ?regulates bone mass and influence osteoblast activity.[1].Dentonin TFA (3-30 μg/ml; 2-24 hours) significantly increases the numbers of cell and shows enhanced promotion of cell adhesion. However, it has no significant differences in terms of numbers of cells adhered[2].Dentonin TFA (3-30 μg/ml; 2-24 hours) enhances osteoblast spreading, it exhibits significantly increased cell areas compared to all other treatment[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Molecular Weight 2652.61
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Formel C109H161F3N30O44
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Synonyms
AC-100 TFA
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Sequence Shortening
TDLQERGDNDISPFSGDGQPFKD
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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Mesenchymal stromal/stem cell osteogenic differentiation
Mesenchymal stromal/stem cells can be induced toward an osteoblast-like lineage in vitro by culture in osteogenic medium containing dexamethasone, ascorbic acid or ascorbate-2-phosphate, and β-glycerophosphate; the differentiation process is commonly evaluated by alkaline phosphatase activity, osteogenic marker expression, collagenous matrix formation, and calcium-rich matrix mineralization. The main readouts are alkaline phosphatase activity as an early osteogenic marker and Alizarin Red S staining as a calcium-deposit readout for mineralized extracellular matrix; Alizarin Red S can be inspected microscopically or extracted and measured colorimetrically at 405 nm.
Reinheit & Dokumentation
Verweise
[1]. Andrew P Sprowson, et al. ASARM-truncated MEPE and AC-100 enhance osteogenesis by promoting osteoprogenitor adhesion. J Orthop Res. 2008 Sep;26(9):1256-62. [Content Brief]
[2]. N Six, et al. Dentonin, a MEPE fragment, initiates pulp-healing response to injury.J Dent Res. 2007 Aug;86(8):780-5. [Content Brief]
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Dentonin TFA Related Classifications
HY-P2633A Related Classifications
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