1. Membrane Transporter/Ion Channel
    Neuronal Signaling
    Metabolic Enzyme/Protease
    Apoptosis
  2. GABA Receptor
    Cytochrome P450
    Apoptosis
  3. Fipronil

Fipronil 

Cat. No.: HY-B0822 Purity: ≥98.0%
COA Handling Instructions

Fipronil is a broad-spectrum insecticide effective against Lepidoptera species as well as thrips, locusts, ants, cockroaches, fleas and ticks. Fipronil selectively inhibits GABA receptor with IC50s of 30 nM and 1600 nM for cockroach and rat GABA receptors, respectively. Glutamate-gated chloride channels (GluCls), which are present in cockroaches but not in mammals, are sensitive to the blocking effect of Fipronil. Fipronil also induces apoptosis in HepG2 cells and promotes the expression of CYP1A1 and CYP3A4 mRNA in human hepatocytes.

For research use only. We do not sell to patients.

Fipronil Chemical Structure

Fipronil Chemical Structure

CAS No. : 120068-37-3

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Description

Fipronil is a broad-spectrum insecticide effective against Lepidoptera species as well as thrips, locusts, ants, cockroaches, fleas and ticks. Fipronil selectively inhibits GABA receptor with IC50s of 30 nM and 1600 nM for cockroach and rat GABA receptors, respectively. Glutamate-gated chloride channels (GluCls), which are present in cockroaches but not in mammals, are sensitive to the blocking effect of Fipronil. Fipronil also induces apoptosis in HepG2 cells and promotes the expression of CYP1A1 and CYP3A4 mRNA in human hepatocytes[1][2].

IC50 & Target

CYP1

 

CYP2

 

CYP3

 

In Vitro

Fipronil (0.1-25 µM; 72 h) dose-dependently induces expression of CYP1A1 and CYP3A4 mRNA in human hepatocytes[1].
Fipronil (0.1-25 µM; 72 h) induces caspase-3/7 activity in a time- and dose-dependent manner, in HepG2 cells (caspase-3/7 activity is an important marker of the cellular apoptotic process)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR[1]

Cell Line: Human hepatocytes
Concentration: 0.1-25 µM
Incubation Time: 72 h
Result: Remarkably increased the expression of CYP1A1 and CYP3A4 mRNA in a dose-dependent manner.

Apoptosis Analysis[1]

Cell Line: HepG2 cells
Concentration: 0.05, 0.1, 0.5, 1, 3.125, 6.25, 12.5, 25, 50 µM
Incubation Time: 24, 48, 72 h
Result: Exhibited time- and dose-dependent induction of caspase-3/7 activity from 0.1 to 6.25 µM, while slowly decreasing from 12.5 to 50 µM.
In Vivo

Fipronil inhibits two types of glutamate-induced chloride desensitizing and non-desensitizing current with IC50 values of 800 and 10 nM, respectively[2].
Fipronil is more selective for cockroach GABA receptors than for rat GABA[2].
Fipronil blocks cockroach GABA receptors in both resting and activated states, with IC50s of 28 and 35 nM, respectively[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

437.15

Formula

C12H4Cl2F6N4OS

CAS No.
SMILES

N#CC1=NN(C2=C(Cl)C=C(C(F)(F)F)C=C2Cl)C(N)=C1S(C(F)(F)F)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (571.89 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2875 mL 11.4377 mL 22.8754 mL
5 mM 0.4575 mL 2.2875 mL 4.5751 mL
10 mM 0.2288 mL 1.1438 mL 2.2875 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.76 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.76 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Fipronil
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