Cat. No.: HY-139142

Simufilam

(Synonyms: PTI-125)

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

Simufilam (PTI-125) is an orally active FLNA modulator. Simufilam restores NMDAR signaling and Arc expression. Simufilam inhibits overactive mTOR signaling by restoring the normal conformation of FLNA, improves insulin sensitivity, reduces Aβ₄₂-induced neuroinflammation and tau protein hyperphosphorylation. Simufilam can be used for research of Alzheimer's disease.

For research use only. We do not sell to patients.

Purity: 99.78%
CAS No.: 1224591-33-6
Formula: C₁₅H₂₁N₃O
Molecular Weight:259.35

Storage:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Simufilam Chemical Structure

CAS No.: 1224591-33-6

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All mTOR Isoforms :

All iGluR Isoforms :

In Vitro

Simufilam (1 nM; 1 h) reduces FLNA-α7nAChR/TLR4 associations, dissociates Aβ₄₂-α7nAChR complexes, restores α7nAChR/NMDAR/IR function, and decreases tau phosphorylation in postmortem human AD brain slices^[2].
Simufilam (1 pM-1 nM; 1 h) dose-dependently reduces FLNA-α7nAChR/TLR4 associations, Aβ₄₂-α7nAChR complexes, tau phosphorylation, and tau nitration, while restoring NMDAR signaling and Arc expression in postmortem human AD hippocampal slices^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Simufilam (10 mg/kg; i.p.; daily; 2 weeks) reduces FLNA-α7nAChR/TLR4 associations, tau phosphorylation, Aβ₄₂ aggregates, and inflammatory cytokines, while restoring α7nAChR/NMDAR/IR function in ICV Aβ₄₂-infused mice^[2].
Simufilam (30 mg/kg/d; p.o.; 2 months) reduces FLNA-α7nAChR/TLR4 associations, tau phosphorylation, Aβ₄₂ deposits, and NFTs, improves NMDAR/IR function and synaptic plasticity, and enhances spatial/working memory and nesting behavior in 3xTg AD mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1224591-33-6
Appearance Solid-Liquid Mixture
Molecular Weight 259.35
Formula C₁₅H₂₁N₃O
Color White to off-white
SMILES

O=C1N(CC2=CC=CC=C2)C3(CCN(C)CC3)NC1
Synonyms

PTI-125
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Journal Impact Factor

J Neurooncol

Single-cell RNA-seq reveals diverse molecular signatures associated with Methotrexate resistance in primary central nervous system lymphoma cells. [Abstract]

2025 Mar;172(1):163-173. PMID: 39636551

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (192.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

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Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.8558 mL

19.2790 mL

38.5579 mL

5 mM

0.7712 mL

3.8558 mL

7.7116 mL

10 mM

0.3856 mL

1.9279 mL

3.8558 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wang HY, et al. Simufilam suppresses overactive mTOR and restores its sensitivity to insulin in Alzheimer's disease patient lymphocytes. Front Aging. 2023 Jun 29;4:1175601. [Content Brief]

[2]. Wang HY, et al. Reducing amyloid-related Alzheimer's disease pathogenesis by a small molecule targeting filamin A. J Neurosci. 2012 Jul 18;32(29):9773-84. [Content Brief]

[3]. Wang HY, et al. PTI-125 binds and reverses an altered conformation of filamin A to reduce Alzheimer's disease pathogenesis. Neurobiol Aging. 2017 Jul;55:99-114. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.8558 mL	19.2790 mL	38.5579 mL	96.3948 mL
	5 mM	0.7712 mL	3.8558 mL	7.7116 mL	19.2790 mL
	10 mM	0.3856 mL	1.9279 mL	3.8558 mL	9.6395 mL
	15 mM	0.2571 mL	1.2853 mL	2.5705 mL	6.4263 mL
	20 mM	0.1928 mL	0.9639 mL	1.9279 mL	4.8197 mL
	25 mM	0.1542 mL	0.7712 mL	1.5423 mL	3.8558 mL
	30 mM	0.1285 mL	0.6426 mL	1.2853 mL	3.2132 mL
	40 mM	0.0964 mL	0.4820 mL	0.9639 mL	2.4099 mL
	50 mM	0.0771 mL	0.3856 mL	0.7712 mL	1.9279 mL
	60 mM	0.0643 mL	0.3213 mL	0.6426 mL	1.6066 mL
	80 mM	0.0482 mL	0.2410 mL	0.4820 mL	1.2049 mL
	100 mM	0.0386 mL	0.1928 mL	0.3856 mL	0.9639 mL

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Simufilam

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Publications Citing Use of MedChemExpress (MCE) Simufilam

All mTOR Isoforms

All iGluR Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Simufilam Related Classifications

HY-139142 Related Classifications