Cat. No.: HY-139142B

Simufilam hydrochloride

(Synonyms: PTI-125 hydrochloride)

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

Simufilam hydrochloride (PTI-125 hydrochloride) is an orally active FLNA modulator. Simufilam hydrochloride restores NMDAR signaling and Arc expression. Simufilam hydrochloride inhibits overactive mTOR signaling by restoring the normal conformation of FLNA, improves insulin sensitivity, reduces Aβ₄₂-induced neuroinflammation and tau protein hyperphosphorylation. Simufilam hydrochloride can be used for research of Alzheimer's disease.

For research use only. We do not sell to patients.

Purity: 99.85%
CAS No.: 2480226-07-9
Formula: C₁₅H₂₂ClN₃O
Molecular Weight:295.81

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Simufilam hydrochloride Chemical Structure

CAS No.: 2480226-07-9

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Biological Activity
- Description
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All mTOR Isoforms :

All iGluR Isoforms :

In Vitro

Simufilam (1 nM; 1 h) hydrochloride reduces FLNA-α7nAChR/TLR4 associations, dissociates Aβ₄₂-α7nAChR complexes, restores α7nAChR/NMDAR/IR function, and decreases tau phosphorylation in postmortem human AD brain slices^[2].
Simufilam (1 pM-1 nM; 1 h) hydrochloride dose-dependently reduces FLNA-α7nAChR/TLR4 associations, Aβ₄₂-α7nAChR complexes, tau phosphorylation, and tau nitration, while restoring NMDAR signaling and Arc expression in postmortem human AD hippocampal slices^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Simufilam (10 mg/kg; i.p.; daily; 2 weeks) hydrochloride reduces FLNA-α7nAChR/TLR4 associations, tau phosphorylation, Aβ₄₂ aggregates, and inflammatory cytokines, while restoring α7nAChR/NMDAR/IR function in ICV Aβ₄₂-infused mice^[2].
Simufilam hydrochloride (30 mg/kg/d; p.o.; 2 months) reduces FLNA-α7nAChR/TLR4 associations, tau phosphorylation, Aβ₄₂ deposits, and NFTs, improves NMDAR/IR function and synaptic plasticity, and enhances spatial/working memory and nesting behavior in 3xTg AD mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 2480226-07-9
Appearance Solid
Molecular Weight 295.81
Formula C₁₅H₂₂ClN₃O
Color White to off-white
SMILES

O=C1N(CC2=CC=CC=C2)C3(CCN(C)CC3)NC1.[H]Cl
Synonyms

PTI-125 hydrochloride
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (1)

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Journal Impact Factor

J Neurooncol

Single-cell RNA-seq reveals diverse molecular signatures associated with Methotrexate resistance in primary central nervous system lymphoma cells. [Abstract]

2025 Mar;172(1):163-173. PMID: 39636551

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (422.57 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (338.05 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.3805 mL

16.9027 mL

33.8055 mL

5 mM

0.6761 mL

3.3805 mL

6.7611 mL

10 mM

0.3381 mL

1.6903 mL

3.3805 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Dilution Calculator

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (8.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (8.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
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Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
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Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wang HY, et al. Simufilam suppresses overactive mTOR and restores its sensitivity to insulin in Alzheimer's disease patient lymphocytes. Front Aging. 2023 Jun 29;4:1175601. [Content Brief]

[2]. Wang HY, et al. Reducing amyloid-related Alzheimer's disease pathogenesis by a small molecule targeting filamin A. J Neurosci. 2012 Jul 18;32(29):9773-84. [Content Brief]

[3]. Wang HY, et al. PTI-125 binds and reverses an altered conformation of filamin A to reduce Alzheimer's disease pathogenesis. Neurobiol Aging. 2017 Jul;55:99-114. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	3.3805 mL	16.9027 mL	33.8055 mL	84.5137 mL
	5 mM	0.6761 mL	3.3805 mL	6.7611 mL	16.9027 mL
	10 mM	0.3381 mL	1.6903 mL	3.3805 mL	8.4514 mL
	15 mM	0.2254 mL	1.1268 mL	2.2537 mL	5.6342 mL
	20 mM	0.1690 mL	0.8451 mL	1.6903 mL	4.2257 mL
	25 mM	0.1352 mL	0.6761 mL	1.3522 mL	3.3805 mL
	30 mM	0.1127 mL	0.5634 mL	1.1268 mL	2.8171 mL
	40 mM	0.0845 mL	0.4226 mL	0.8451 mL	2.1128 mL
	50 mM	0.0676 mL	0.3381 mL	0.6761 mL	1.6903 mL
	60 mM	0.0563 mL	0.2817 mL	0.5634 mL	1.4086 mL
	80 mM	0.0423 mL	0.2113 mL	0.4226 mL	1.0564 mL
	100 mM	0.0338 mL	0.1690 mL	0.3381 mL	0.8451 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Simufilam hydrochloride

1 Cited Publications

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Simufilam hydrochloride

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Publications Citing Use of MedChemExpress (MCE) Simufilam hydrochloride

All mTOR Isoforms

All iGluR Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Simufilam hydrochloride Related Classifications

HY-139142B Related Classifications