Referencia número: HY-112438

MF-094

Name: MF-094
Brand: MedChemExpress
SKU: HY-112438
Price: 110.00 USD
Availability: InStock
Rating: 5 (9 reviews)

9 Cited Publications

Revisión del cliente

Based on 9 publication(s) in Google Scholar

MF-094 is a potent and selective USP30 inhibitor with an IC₅₀ of 120 nM. MF-094 increases protein ubiquitination and accelerates mitophagy.

Para uso exclusivo en investigación. No vendemos a pacientes.

Pureza : 99.75%
No. CAS: 2241025-68-1
Fòrmula: C₃₀H₃₇N₃O₄S
Peso molecular:535.70

Almacenamiento:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

MF-094 Chemical Structure

No. CAS: 2241025-68-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (9)
Solvente y solubilidad
- In Vitro
- In Vivo
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

MF-094 is a potent and selective USP30 inhibitor with an IC₅₀ of 120 nM. MF-094 increases protein ubiquitination and accelerates mitophagy^[1].

IC₅₀ & Target

IC50: 120 nM (USP30)^[1]

Ver todos los productos específicos de isoformas Deubiquitinase :

In Vitro

MF-094 has <30% inhibitory activity for a panel of 22 USPs assays at 10 uM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Mitophagy Solutions

Chemical Information

No. CAS 2241025-68-1
Appearance Solid
Peso molecular 535.70
Fòrmula C₃₀H₃₇N₃O₄S
Color White to yellow
SMILES

O=C(NC1=CC=CC2=C1C=CC=C2S(=O)(NC(C)(C)C)=O)[C@@H](NC(C3CCCCC3)=O)CC4=CC=CC=C4
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (9)

All Bio/Physico-chemical AssayCell Proliferation/Viability AssayApoptosis AnalysisWB

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Journal Impact Factor

Cancer Discov

Mitophagy promotes resistance to BH3 mimetics in acute myeloid leukemia. [Abstract]

2023 Jul 7;13(7):1656-1677. PMID: 37088914

MF-094 purchased from MedChemExpress. Usage Cited in: Cancer Discov. 2023 Jul 7;13(7):1656-1677. [Abstract]

MF-094 (10 μM; 29 h) decreased the Caspase 3/7 activaty whitch were induced by AZD5991 (MCL1i, 1 μM, 8 hr) in MOLM-13 parental cells.
Nat Commun

USP2 inhibition unleashes CD47-restrained phagocytosis and enhances anti-tumor immunity. [Abstract]

2025 May 16;16(1):4564. PMID: 40379682
Nat Commun

USP8 inhibition reshapes an inflamed tumor microenvironment that potentiates the immunotherapy. [Abstract]

2022 Mar 31;13(1):1700. PMID: 35361799
Redox Biol

USP20 governs tyrosine kinase inhibitors resistance through ferroptosis evasion by targeting GPX4 in cancers. [Abstract]

2026 May:92:104086. PMID: 41844497

MF-094 purchased from MedChemExpress. Usage Cited in: Redox Biol. 2026 May:92:104086. [Abstract]

MF-094 (10 μM; 24 h) showed no effect on GPX4 protein expression in sorafenib-resistant 769-P and A549 cells.
J Immunother Cancer

CD47 destabilization via manipulating the SPOP-USP2 axis augments macrophage phagocytosis and cancer immunotherapy. [Abstract]

2026 Jan 14;14(1):e013498. PMID: 41534899
Transl Stroke Res

Inhibition of USP30 Promotes Mitophagy by Regulating Ubiquitination of MFN2 by Parkin to Attenuate Early Brain Injury After SAH. [Abstract]

2025 Apr;16(2):448-466. PMID: 38147294

MF-094 purchased from MedChemExpress. Usage Cited in: Transl Stroke Res. 2025 Apr;16(2):448-466. [Abstract]

MF-094 (100, 140, 180, 220, 260 nM; 24 h) showed a maximum survival rate at 180 nM on primary neurons.

MF-094 purchased from MedChemExpress. Usage Cited in: Transl Stroke Res. 2025 Apr;16(2):448-466. [Abstract]

MF-094 (180 nM) had a significant inhibitory effect on USP30 after SAH in primary neurons.

MF-094 purchased from MedChemExpress. Usage Cited in: Transl Stroke Res. 2025 Apr;16(2):448-466. [Abstract]

MF-094 (180 nM) significantly reduced apoptosis in neurons after subarachnoid hemorrhage (SAH) in primary neurons.

MF-094 purchased from MedChemExpress. Usage Cited in: Transl Stroke Res. 2025 Apr;16(2):448-466. [Abstract]

MF-094 (180 nM) had a substantial reduction in ROS levels after subarachnoid hemorrhage (SAH) in primary neurons.

MF-094 purchased from MedChemExpress. Usage Cited in: Transl Stroke Res. 2025 Apr;16(2):448-466. [Abstract]

MF-094 (5 mg/kg; i.c.v.; daily) decreased the protein expression ofUSP30, MFN2, which were induced by ASH, and increased the protein expression of PARKIN and PINK1 in the brain tissue of mice with ASH treatment.
Anim Cells Syst

Blocking SLC7A11 attenuates the proliferation of esophageal squamous cell carcinoma cells. [Abstract]

2024 May 11;28(1):237-250. PMID: 38741950
Cell Press Blue

2026 Jul 3.
Bioengineered

Increased FUN14 domain containing 1 (FUNDC1) ubiquitination level inhibits mitophagy and alleviates the injury in hypoxia-induced trophoblast cells. [Abstract]

2022 Feb;13(2):3620-3633. PMID: 34699308

Solvente y solubilidad

In Vitro:

DMSO : 125 mg/mL (233.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8667 mL

9.3336 mL

18.6672 mL

5 mM

0.3733 mL

1.8667 mL

3.7334 mL

10 mM

0.1867 mL

0.9334 mL

1.8667 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 5 mg/mL (9.33 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.08 mg/mL (3.88 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (3.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (277 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Kluge AF, et al. Novel highly selective inhibitors of ubiquitin specific protease 30 (USP30) accelerate mitophagy. Bioorg Med Chem Lett. 2018 Aug 15;28(15):2655-2659. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.8667 mL	9.3336 mL	18.6672 mL	46.6679 mL
	5 mM	0.3733 mL	1.8667 mL	3.7334 mL	9.3336 mL
	10 mM	0.1867 mL	0.9334 mL	1.8667 mL	4.6668 mL
	15 mM	0.1244 mL	0.6222 mL	1.2445 mL	3.1112 mL
	20 mM	0.0933 mL	0.4667 mL	0.9334 mL	2.3334 mL
	25 mM	0.0747 mL	0.3733 mL	0.7467 mL	1.8667 mL
	30 mM	0.0622 mL	0.3111 mL	0.6222 mL	1.5556 mL
	40 mM	0.0467 mL	0.2333 mL	0.4667 mL	1.1667 mL
	50 mM	0.0373 mL	0.1867 mL	0.3733 mL	0.9334 mL
	60 mM	0.0311 mL	0.1556 mL	0.3111 mL	0.7778 mL
	80 mM	0.0233 mL	0.1167 mL	0.2333 mL	0.5833 mL
	100 mM	0.0187 mL	0.0933 mL	0.1867 mL	0.4667 mL

Bioz

Keywords

MF-094

9 Cited Publications

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MF-094

Publications Citing Use of MedChemExpress (MCE) MF-094

Ver todos los productos específicos de isoformas Deubiquitinase