Pentostatin
Based on 10 publication(s) in Google Scholar
Pentostatin (CI-825; Deoxycoformycin) is an irreversible inhibitor of adenosine deaminase with Ki of 2.5 pM.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.82%
- No. CAS: 53910-25-1
- Fòrmula: C11H16N4O4
- Peso molecular:268.27
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Pentostatin
More- Sci Transl Med. 2020 May 6;12(542):eaba0769. [Abstract]
- J Nanobiotechnology. 2019 Mar 29;17(1):45. [Abstract]
- Adv Sci (Weinh). 2024 Aug;11(30):e2306514. [Abstract]
- Virulence. 2018;9(1):1509-1520. [Abstract]
- PLoS Pathog. 2020 Mar 16;16(3):e1008341. [Abstract]
- J Neurochem. 2019 Aug;150(3):282-295. [Abstract]
- Neurobiol Stress. 2021 Sep 11;15:100396. [Abstract]
- J Immunol. 2022 Jul 15;209(2):401-411. [Abstract]
- Authorea. 2025 Oct 7.
- bioRxiv. 2023 Sep 12:2023.09.09.557001. [Abstract]
Customer Validation & Images
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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Cell Proliferation/Viability Assay
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WB
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WB
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Actividad biológica
Ki: 2.5 pM (adenosine deaminase)
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| L1210 | IC50 |
0.25 μM
Compound: pentostatin
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Compound was evaluated for the cytotoxicity against L1210 cells in presence of coformycin
Compound was evaluated for the cytotoxicity against L1210 cells in presence of coformycin
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[PMID: 3489838] |
| L1210 | IC50 |
1.9 μM
Compound: pentostatin
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Compound was evaluated for the cytotoxicity against L1210 cells in absence of coformycin
Compound was evaluated for the cytotoxicity against L1210 cells in absence of coformycin
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[PMID: 3489838] |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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No. CAS 53910-25-1
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Appearance Solid
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Peso molecular 268.27
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Fòrmula C11H16N4O4
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Color White to off-white
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SMILES
O[C@H]1C(N=CN2[C@H](O[C@@H]3CO)C[C@@H]3O)=C2NC=NC1
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Synonyms
CI-825; Deoxycoformycin
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (10)
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Journal Impact Factor
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Most Recent
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Sci Transl Med
2020 May 6;12(542):eaba0769. PMID: 32376767 -
J Nanobiotechnology
Role of A2B adenosine receptor-dependent adenosine signaling in multi-walled carbon nanotube-triggered lung fibrosis in mice. [Abstract]2019 Mar 29;17(1):45. PMID: 30922349 -
Adv Sci (Weinh)
Targeting Adenosine A2b Receptor Promotes Penile Rehabilitation of Refractory Erectile Dysfunction. [Abstract]2024 Aug;11(30):e2306514. PMID: 38874549
Pentostatin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Aug;11(30):e2306514. [Abstract]
Pentostatin (Deoxycoformycin; DCF) (0.2 mg/kg; i.c.) elevated ICPs of C57BL/6 mice.
Pentostatin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Aug;11(30):e2306514. [Abstract]
Pentostatin (Deoxycoformycin; DCF) (0.2 mg/kg; i.c.) elevated AUCs of C57BL/6 mice.
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Virulence
Streptococcus suis synthesizes deoxyadenosine and adenosine by 5'-nucleotidase to dampen host immune responses. [Abstract]2018;9(1):1509-1520. PMID: 30221577
Pentostatin purchased from MedChemExpress. Usage Cited in: Virulence. 2018;9(1):1509-1520. [Abstract]
The viability of RAW 264.7 cells treated with or without Pentostatin (Deoxycoformycin; dCF) (100 μM).
Pentostatin purchased from MedChemExpress. Usage Cited in: Virulence. 2018;9(1):1509-1520. [Abstract]
Pentostatin (Deoxycoformycin; dCF) (100 μM; 12-24 h) induced a detectable band of active caspase-3 in mouse macrophages RAW 264.7 treated with dAdo.
Pentostatin purchased from MedChemExpress. Usage Cited in: Virulence. 2018;9(1):1509-1520. [Abstract]
Pentostatin (Deoxycoformycin; dCF) (100 μM; 12 h) induced a detectable band of active caspase-3 in mouse macrophages RAW 264.7 treated with tNT+dAMP.
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PLoS Pathog
Identification of amitriptyline HCl, flavin adenine dinucleotide, azacitidine and calcitriol as repurposing drugs for influenza A H5N1 virus-induced lung injury. [Abstract]2020 Mar 16;16(3):e1008341. PMID: 32176725 -
J Neurochem
Metabolomics-driven identification of adenosine deaminase as therapeutic target in a mouse model of Parkinson's disease. [Abstract]2019 Aug;150(3):282-295. PMID: 31121068 -
Neurobiol Stress
2021 Sep 11;15:100396. PMID: 34568523 -
J Immunol
Adenosine Alleviates Necrotizing Enterocolitis by Enhancing the Immunosuppressive Function of Myeloid-Derived Suppressor Cells in Newborns. [Abstract]2022 Jul 15;209(2):401-411. PMID: 35777852 -
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bioRxiv
Multiplexed single-cell lineage tracing of mitotic kinesin inhibitor resistance in glioblastoma. [Abstract]2023 Sep 12:2023.09.09.557001. PMID: 37745469
Solvente y solubilidad
H2O : 100 mg/mL (372.75 mM; Need ultrasonic)
DMSO : ≥ 50 mg/mL (186.38 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.75 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.75 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: Saline
Solubility: 100 mg/mL (372.75 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocolo
All recipient dogs are conditioned for transplantation by 920 cGy TBI at 7 cGy/minute using a linear accelerator. Dogs in group A1receive ECP administered on days −2 and −1 with TBI on day 0 and dogs in group A2receive ECP on days −6 and −5, intravenous (IV) infusion of pentostatin at a dose of 4 mg/m2 on days −4 and −3, and TBI on day 0. Donor marrow cells from DLA-nonidentical donors are aspirated under general anesthesia through needles inserted into humeri and femora and stored in heparinized tissue culture medium at 4°C for no more than 6 hours. Within 4 hours of TBI, harvested marrow cells are infused IV into recipients at a median dose of 2.9 (range, 1.9 to 6.1) ×108 total nucleated cells (TNC)/kg. The day of marrow grafting is designated as day 0. In addition to marrow graft, recipients are given IV infusions of peripheral blood buffy coat cells obtained by leukapheresis from the marrow donor on days 1 and 2, at a median dose of 2.3 (range, 1.2 to 6.9) ×108 TNC/kg to ensure consistent hematopoietic engraftment. MTX, at a dose of 0.4 mg/kg intravenously is used as postgrafting immunosuppression and administered on days +1, +3, +6 and +11, then weekly thereafter until day 102.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureza y Documentación
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Ficha de datos (280 KB)
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SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Bethge WA, et al. Extracorporeal photopheresis combined with pentostatin in the conditioning regimen for canine hematopoietic cell transplantation does not prevent GVHD. Bone Marrow Transplant. 2014 Sep;49(9):1198-204. [Content Brief]
[2]. Dalla Rosa L, et al. Cordycepin (3'-deoxyadenosine) pentostatin (deoxycoformycin) combination treatment of mice experimentally infected with Trypanosoma evansi. Parasitology. 2013 Apr;140(5):663-71. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 3.7275 mL | 18.6377 mL | 37.2755 mL | 93.1887 mL |
| 5 mM | 0.7455 mL | 3.7275 mL | 7.4551 mL | 18.6377 mL | |
| 10 mM | 0.3728 mL | 1.8638 mL | 3.7275 mL | 9.3189 mL | |
| 15 mM | 0.2485 mL | 1.2425 mL | 2.4850 mL | 6.2126 mL | |
| 20 mM | 0.1864 mL | 0.9319 mL | 1.8638 mL | 4.6594 mL | |
| 25 mM | 0.1491 mL | 0.7455 mL | 1.4910 mL | 3.7275 mL | |
| 30 mM | 0.1243 mL | 0.6213 mL | 1.2425 mL | 3.1063 mL | |
| 40 mM | 0.0932 mL | 0.4659 mL | 0.9319 mL | 2.3297 mL | |
| 50 mM | 0.0746 mL | 0.3728 mL | 0.7455 mL | 1.8638 mL | |
| 60 mM | 0.0621 mL | 0.3106 mL | 0.6213 mL | 1.5531 mL | |
| 80 mM | 0.0466 mL | 0.2330 mL | 0.4659 mL | 1.1649 mL | |
| 100 mM | 0.0373 mL | 0.1864 mL | 0.3728 mL | 0.9319 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Pentostatin Related Classifications
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