Referencia número: HY-155747

FDW028

2 Cited Publications

Revisión del cliente

Based on 2 publication(s) in Google Scholar

FDW028 a potent and highly selective FUT8 inhibitor. FUT8 exhibits potent anti-tumor activity by defucosylation and impelling lysosomal degradation of B7-H3 through the chaperone-mediated autophagy (CMA) pathway. FDW028 can be used for metastatic colorectal cancer (mCRC) research.

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Pureza: 99.41%
No. CAS: 2768426-49-7
Fòrmula: C₂₂H₂₄N₆O
Peso molecular:388.47

Almacenamiento:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

FDW028 Chemical Structure

No. CAS: 2768426-49-7

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Actividad biológica
- Descripciòn
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvente y solubilidad
- In Vitro
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

Ver todos los productos específicos de isoformas Akt :

Ver todos los productos específicos de isoformas mTOR :

In Vitro

FDW028 (50 μM, 72 hours) promotes defucosylation and lysosomal degradation of B7-H3 and inhibits the AKT/MTOR pathway^[1].
FDW028 (50 μM, 72 hours) promotes B7-H3 and LAMP1 colocalization, facilitating lysosomal degradation of B7-H3^[1].
FDW028 (0.2–100 μM, 72 hours) significantly inhibits proliferation of SW480 and HCT-8 cells with IC50 values of 5.95 μM and 23.78 μM^[1].
FDW028 (50 μM, 72 hours) significantly inhibits migration in SW480 and HCT-8 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[1]

Cell Line:

SW480 cells, HCT-8 cells
Concentration:

0.2–100 μM
Incubation Time:

72 h
Result:

Promoted the colocalization of B7-H3 and LAMP1 in lysosomes.

Cell Proliferation Assay^[1]

Cell Line:

SW480 cells, HCT-8 cells
Concentration:

50 μM
Incubation Time:

72 h
Result:

Inhibited cell proliferation, with IC₅₀ values of 5.95 μM and 23.78 μM for SW480 and HCT-8, respectively.

Western Blot Analysis^[1]

Cell Line:

SW480 cells, HCT-8 cells
Concentration:

50 μM
Incubation Time:

72 h
Result:

Promoted defucosylation of B7-H3, facilitated lysosomal degradation, and inhibited the AKT/MTOR pathway.

Cell Migration Assay ^[1]

Cell Line:

SW480 cells, HCT-8 cells
Concentration:

50 μM
Incubation Time:

72 h
Result:

Inhibited the migration ability of SW480 and HCT-8 cells.

In Vivo

FDW028 (10 or 20 mg/kg, i.v. every other day) exhibits significant anti-tumor activity in the SW480 xenograft mouse model^[1].
FDW028 (20 mg/kg, i.v. every other day) significantly prolongs survival in the Mc38 pulmonary metastasis model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Colorectal cancer cell line SW480 xenograft mouse model (C57BL/6 mice, 4-6 weeks)^[1]
Dosage:

10 mg/kg, 20 mg/kg
Administration:

Intravenous injection (i.v.), every other day, tumor size and body weight were measured every other day during the experiment
Result:

Inhibited tumor growth, comparable to 5-Fu (HY-90006), and did not cause significant weight loss.

Animal Model:

Mc38 colorectal cancer pulmonary metastasis mouse model (C57BL/6 mice, 4-6 weeks)^[1]
Dosage:

20 mg/kg
Administration:

Intravenous injection (i.v.), every other day, were measured every other day during the experiment
Result:

Significantly prolonged the survival of tumor-bearing mice.

Chemical Information

No. CAS 2768426-49-7
Appearance Solid
Peso molecular 388.47
Fòrmula C₂₂H₂₄N₆O
Color White to off-white
SMILES

OC1=CC(CNCC2=CC=CC=C2)=NC3=NC(NCC4=CC=C(CC)C=C4)=NN31
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (2)

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Journal Impact Factor

Int J Biol Macromol

Genome-wide CRISPR screen identifies ALG5, ALG6, NF2, and FUT8 as key host proteins involved in transmissible gastroenteritis virus infection. [Abstract]

2025 Dec;333(Pt 2):148770. PMID: 41205942
PLoS Pathog

FUT8-mediated core fucosylation of receptor APN drives entry of multiple alphacoronaviruses. [Abstract]

2026 May 18;22(5):e1014227. PMID: 42149951

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (257.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

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Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5742 mL

12.8710 mL

25.7420 mL

5 mM

0.5148 mL

2.5742 mL

5.1484 mL

10 mM

0.2574 mL

1.2871 mL

2.5742 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (275 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Wang M, et al. FDW028, a novel FUT8 inhibitor, impels lysosomal proteolysis of B7-H3 via chaperone-mediated autophagy pathway and exhibits potent efficacy against metastatic colorectal cancer. Cell Death Dis. 2023 Aug 3;14(8):495. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5742 mL	12.8710 mL	25.7420 mL	64.3550 mL
	5 mM	0.5148 mL	2.5742 mL	5.1484 mL	12.8710 mL
	10 mM	0.2574 mL	1.2871 mL	2.5742 mL	6.4355 mL
	15 mM	0.1716 mL	0.8581 mL	1.7161 mL	4.2903 mL
	20 mM	0.1287 mL	0.6436 mL	1.2871 mL	3.2178 mL
	25 mM	0.1030 mL	0.5148 mL	1.0297 mL	2.5742 mL
	30 mM	0.0858 mL	0.4290 mL	0.8581 mL	2.1452 mL
	40 mM	0.0644 mL	0.3218 mL	0.6436 mL	1.6089 mL
	50 mM	0.0515 mL	0.2574 mL	0.5148 mL	1.2871 mL
	60 mM	0.0429 mL	0.2145 mL	0.4290 mL	1.0726 mL
	80 mM	0.0322 mL	0.1609 mL	0.3218 mL	0.8044 mL
	100 mM	0.0257 mL	0.1287 mL	0.2574 mL	0.6436 mL

Keywords

FDW028

2 Cited Publications

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FDW028

Kinase Inhibitor Library

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Mitochondrial Protection Compound Library

Highly Selective Inhibitors Library

Cell Death Library

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Bioactive Compound Library

Anti-Gastric Cancer Compound Library

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Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) FDW028

Ver todos los productos específicos de isoformas Akt

Ver todos los productos específicos de isoformas mTOR

Actividad biológica

Chemical Information

Publications (2)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

FDW028 Related Classifications

HY-155747 Related Classifications