Fipronil
Based on 1 Customer Validation
Fipronil is a broad-spectrum insecticide effective against Lepidoptera species as well as thrips, locusts, ants, cockroaches, fleas and ticks. Fipronil selectively inhibits GABA receptor with IC50s of 30 nM and 1600 nM for cockroach and rat GABA receptors, respectively. Glutamate-gated chloride channels (GluCls), which are present in cockroaches but not in mammals, are sensitive to the blocking effect of Fipronil. Fipronil also induces apoptosis in HepG2 cells and promotes the expression of CYP1A1 and CYP3A4 mRNA in human hepatocytes.
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- Pureza: 99.49%
- No. CAS: 120068-37-3
- Fòrmula: C12H4Cl2F6N4OS
- Peso molecular:437.15
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Almacenamiento:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
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Help & FAQs
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Actividad biológica
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CYP1 |
CYP2 |
CYP3 |
Fipronil (0.1-25 µM; 72 h) dose-dependently induces expression of CYP1A1 and CYP3A4 mRNA in human hepatocytes[1].
Fipronil (0.1-25 µM; 72 h) induces caspase-3/7 activity in a time- and dose-dependent manner, in HepG2 cells (caspase-3/7 activity is an important marker of the cellular apoptotic process)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human hepatocytes
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Concentration:0.1-25 µM
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Incubation Time:72 h
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Result:Remarkably increased the expression of CYP1A1 and CYP3A4 mRNA in a dose-dependent manner.
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Cell Line:HepG2 cells
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Concentration:0.05, 0.1, 0.5, 1, 3.125, 6.25, 12.5, 25, 50 µM
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Incubation Time:24, 48, 72 h
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Result:Exhibited time- and dose-dependent induction of caspase-3/7 activity from 0.1 to 6.25 µM, while slowly decreasing from 12.5 to 50 µM.
Fipronil is more selective for cockroach GABA receptors than for rat GABA[2].
Fipronil blocks cockroach GABA receptors in both resting and activated states, with IC50s of 28 and 35 nM, respectively[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 120068-37-3
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Appearance Solid
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Peso molecular 437.15
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Fòrmula C12H4Cl2F6N4OS
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Color White to off-white
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SMILES
N#CC1=NN(C2=C(Cl)C=C(C(F)(F)F)C=C2Cl)C(N)=C1S(C(F)(F)F)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvente y solubilidad
DMSO : 250 mg/mL (571.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (278 KB)
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SDS (728 KB)
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- Italian - IT (728 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Das PC, et al. Fipronil induces CYP isoforms and cytotoxicity in human hepatocytes. Chem Biol Interact. 2006 Dec 15;164(3):200-14. [Content Brief]
[2]. Narahashi T, et al. Glutamate-activated chloride channels: Unique fipronil targets present in insects but not in mammals. Pestic Biochem Physiol. 2010 Jun 1;97(2):149-152. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2875 mL | 11.4377 mL | 22.8754 mL | 57.1886 mL |
| 5 mM | 0.4575 mL | 2.2875 mL | 4.5751 mL | 11.4377 mL | |
| 10 mM | 0.2288 mL | 1.1438 mL | 2.2875 mL | 5.7189 mL | |
| 15 mM | 0.1525 mL | 0.7625 mL | 1.5250 mL | 3.8126 mL | |
| 20 mM | 0.1144 mL | 0.5719 mL | 1.1438 mL | 2.8594 mL | |
| 25 mM | 0.0915 mL | 0.4575 mL | 0.9150 mL | 2.2875 mL | |
| 30 mM | 0.0763 mL | 0.3813 mL | 0.7625 mL | 1.9063 mL | |
| 40 mM | 0.0572 mL | 0.2859 mL | 0.5719 mL | 1.4297 mL | |
| 50 mM | 0.0458 mL | 0.2288 mL | 0.4575 mL | 1.1438 mL | |
| 60 mM | 0.0381 mL | 0.1906 mL | 0.3813 mL | 0.9531 mL | |
| 80 mM | 0.0286 mL | 0.1430 mL | 0.2859 mL | 0.7149 mL | |
| 100 mM | 0.0229 mL | 0.1144 mL | 0.2288 mL | 0.5719 mL |
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