INY-05-040
Based on 1 publication(s) in Google Scholar
INY-05-040 is an AKT degrader that can selectively and quickly degrade all three AKT isoforms. INY-05-040 exhibits anti-cancer activity. INY-05-040 can inhibit downstream signaling and cell proliferation in 288 cancer cell lines. INY-05-040 can suppress AKT signaling and induces the stress mitogen-activated protein kinase (MAPK) c-Jun N-terminal kinase (JNK). INY-05-040 is effective for breast cancer lines with a low-baseline activation of stress MAPK pathway. INY-05-040 can be studied for anti-cancer research.
(Pink: Akt ligand (HY-130988); Blue: ligand (HY-112078); Black: linker (HY-W009413)).
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 98.54%
- No. CAS: 2503018-29-7
- Fòrmula: C55H76ClN9O6S
- Peso molecular:1026.77
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Almacenamiento:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) INY-05-040
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Actividad biológica
Descripciòn
In Vitro
INY-05-040 (10-1000 nM, 24 h) demonstrates AKT signaling suppression in T47D cells[1].
INY-05-040 (100 nM, 0-72 h) results in reduced levels of total ribosomal S6 protein and sustained AKT reduction and suppression of downstream signaling in T47D cells[1].
INY-05-040 (100 nM, 10 h) causes differential abundance of 1394 transcripts in T47D cell lines[1].
INY-05-040 (100nM, 8-120 h) induces sustained phosphorylation of the JNK target c-Jun at Ser73 and an increase in total c-Jun protein levels, which is a marker for JNK activation in BT-474 and T47D cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
INY-05-040 (30-40 mg/kg, i.p., single dose) results in a prolonged AKT inhibition in BT474 subcutaneous tumor xenograft mice model[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Breast Cancer Modeling
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- MTT Cell Proliferation Assay
Chemical Information
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No. CAS 2503018-29-7
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Appearance Solid
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Peso molecular 1026.77
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Fòrmula C55H76ClN9O6S
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Color White to off-white
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SMILES
C[C@H]1C2=C(N3CCN(CC3)C([C@@H](C4=CC=C(C=C4)Cl)CNCCCCCCCCCCC(N[C@@H](C(C)(C)C)C(N5[C@@H](C[C@H](C5)O)C(N[C@H](C6=CC=C(C7=C(C)N=CS7)C=C6)C)=O)=O)=O)=O)N=CN=C2[C@@H](C1)O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvente y solubilidad
In Vitro:
DMSO : ≥ 100 mg/mL (97.39 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[2]. Chen, C., et al., (2024). Down-Regulation of AKT Proteins Slows the Growth of Mutant-KRAS Pancreatic Tumors. Cells, 13(12), 1061. [Content Brief]
[3]. Wu, Y., et al., (2025). Pharmacodynamics of Akt drugs revealed by a kinase-modulated bioluminescent indicator. Nature chemical biology, 21(8), 1194–1204. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.9739 mL | 4.8696 mL | 9.7393 mL | 24.3482 mL |
| 5 mM | 0.1948 mL | 0.9739 mL | 1.9479 mL | 4.8696 mL | |
| 10 mM | 0.0974 mL | 0.4870 mL | 0.9739 mL | 2.4348 mL | |
| 15 mM | 0.0649 mL | 0.3246 mL | 0.6493 mL | 1.6232 mL | |
| 20 mM | 0.0487 mL | 0.2435 mL | 0.4870 mL | 1.2174 mL | |
| 25 mM | 0.0390 mL | 0.1948 mL | 0.3896 mL | 0.9739 mL | |
| 30 mM | 0.0325 mL | 0.1623 mL | 0.3246 mL | 0.8116 mL | |
| 40 mM | 0.0243 mL | 0.1217 mL | 0.2435 mL | 0.6087 mL | |
| 50 mM | 0.0195 mL | 0.0974 mL | 0.1948 mL | 0.4870 mL | |
| 60 mM | 0.0162 mL | 0.0812 mL | 0.1623 mL | 0.4058 mL | |
| 80 mM | 0.0122 mL | 0.0609 mL | 0.1217 mL | 0.3044 mL |
INY-05-040 Related Classifications
HY-160469 Related Classifications
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